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Citations for Santa Cruz :
1351 - 1400 of 8048 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... anti-HA (rabbit; 1:100 IF, 1:1000 WB; Santa Cruz sc-805; RRID: AB_631618).
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bioRxiv - Immunology 2024Quote: ... Primary antibodies (ASC 1:200, AdipoGen: AG25B0006C100; LC3B 1:20, Santa Cruz Biotechnology: sc-271625) were diluted in 1.5% BSA and incubated overnight with fixed cells at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... TJA9 diluted 1:1000) and mouse anti-α-actinin (1:1000, sc-17829, Santa Cruz). Detection was performed with Pierce ECL reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated with primary antibodies (rabbit anti-DPP9, Abcam, ab42080, 1:250; mouse anti-NeuN, Millipore ABN78B, 1:500; mouse anti-NPY, Santa Cruz, sc-133080, 1:500) diluted in blocking buffer for 24 h at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies used were as follows: anti-Wilms’ tumor-1 (WT1; 1:400; ab89901, Abcam plc. / 1:100; sc-7385, Santa Cruz Biotechnology Inc., TX, USA), anti-cytokeratin 8 (CK8 (TROMA-I) ...
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bioRxiv - Cell Biology 2024Quote: ... and a 1:100 dilution of anti-stage-specific embryonic antigen-1 (SSEA-1) mouse IgM antibody (sc-2170; Santa Cruz Biotechnology, Dallas, TX, USA) with 1% blocking reagent TNT for 1 hour on ice ...
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bioRxiv - Physiology 2024Quote: ... Samples were incubated with primary antibodies (Connexin 43: C6219, Sigma-Aldrich, dilution 1:500; Connexin 37: ARP36603-P050, Aviva, dilution 1:300; Dab2: sc-136964, Santa Cruz, dilution: 1:400) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... with the respective primary antibody (KRAS-LS-Bio, LS-C175665; 1:4000; α-SMA-Abcam, ab7817, 1:250; GAPDH-Santa Cruz Biotechnology, sc-47724, 1:10000), diluted in 5% non-fat milk in PBS+ 0.5% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... Indicated antibodies (anti-Flag, 1:2000, 12793S, Cell Signaling Technology; anti-Rabbit-IgG, 1:2000, G-21234, Thermo Scientific; anti-β-actin, 1:4000, SC-47778, Santa Cruz Biotechnology) and HRP substrate (K-12042-D10 ...
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bioRxiv - Developmental Biology 2023Quote: ... pH 8.0) for 1 hour at room temperature and incubated overnight in primary antibody (1:100 anti-Dnmt1 polyclonal, Santa Cruz, 1:1000 H3 polyclonal, Santa Cruz) diluted in blocking buffer at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation at 13,200 g for 15 min at 4°C, equal amounts of cell lysates were preincubated with mouse IgG (Applygene, C2118) and Protein A/G agarose beads (Santa Cruz, sc-2003) for 4 h at 4°C and then clarified by centrifugation ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Biochemistry 2021Quote: ... A precleaning step of the samples was performed by adding 50 μl of Protein G-Plus agarose beads suspension (Santa Cruz Biotechnology, INC) to 1 ml of each sample (Chimera ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and identified by immunoblotting with a mouse anti-green fluorescent protein (GFP) (Santa Cruz Biotechnology, Santa Cruz, CA), or anti-Atg13 [175] antibody to detect tagged or endogenous proteins ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653) in the dark at room temperature for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... The epitope-tagged Pum and α-tubulin proteins were detected by successive incubation with anti-Myc 9E10 monoclonal primary (SC-40, Santa Cruz Biotechnology) and anti-α-tubulin B-5-1-2 monoclonal primary (T5168 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining supernatant was subjected to immunoprecipitation using 15 μl of mouse monoclonal antibody against green fluorescent protein (catalog no. sc-9996, Santa Cruz, CA, USA). The immunoprecipitate was gently shaken overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Co-IP was performed using anti-PNKP (BioBharati Life Science, BB-AB0105) antibody with Protein A/G PLUS agarose beads (Santa Cruz Biotechnology, sc2003,) overnight ...
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FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5bioRxiv - Neuroscience 2024Quote: ... beads were resuspended in Laemmli sample buffer and protein were eluted by boiling 10 minutes at 90°C and analyzed by SDS-PAGE and immunoblotting using FUS (Santa Cruz sc-47711), ETV5 (Proteintech 13011-1-AP ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 500 μg of the supernatant proteins were incubated with 2 μg of mouse monoclonal antibody raised against SNAP-25 (Santa Cruz, sc-390814) or Syntaxin1a (MilliporeSigma ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal IgG1 γ-actin antibody (1-24: sc- 65635, Santa Cruz Biotechnology, Inc., 1:100). Alexa Fluor-488 ...
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bioRxiv - Cancer Biology 2020Quote: ... NR5A2 (Invitrogen; #DA5-28347, western blot dilution 1:1000, IHC/IF dilution 1:100; Santa Cruz; #sc-393369 ...
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bioRxiv - Neuroscience 2020Quote: ... and for 1 hour with monoclonal mouse anti-GAPDH antibody (sc-32233 Santa Cruz, 1:3000) as loading control ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was probed with 1:5000 dilution of anti-GFP antibody (1:400; Santa Cruz) to detect YFP-tagged p53 DBDWT and p53 DBDR248W.
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used: Myosin Heavy chain 1/2/4/6 (1:500, Santa Cruz) and secondary antibodies conjugated with Alexa488 (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... from Bethyl Laboratories, anti-p53 (clone DO7 1:2000) and anti-NOXA (114C307, 1:1000) from Santa Cruz, anti-ATM (#ab32420 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (rabbit anti-TRPV4, 1:1000, Lifespan Biosciences, mouse anti-flotillin, 1:200, Santa Cruz; mouse anti-caveolin ...
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bioRxiv - Cancer Biology 2020Quote: ... dilution: 1:2000) or normal mouse control immunoglobulin (sc-3877, Santa Cruz Biotechnology; dilution: 1:2000). The protein precipitates were separated by SDS-PAGE followed by Coomassie staining ...
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bioRxiv - Cell Biology 2020Quote: ... The indicated primary antibody (Tomm70A (Sigma HPA048020, 1:400 and Santa Cruz sc-390545, 1:200), Tomm20 (Millipore MABT116 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse α-pan 14-3-3 (sc-1657, Santa Cruz Biotechnology, 1:1000 to 1:10000), α-Flag® M2-HRP (A8592 ...
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bioRxiv - Cell Biology 2022Quote: ... with the primary antibodies against GPx-1/2 (1:1000, sc-133152, RRID: AB_2112240, Santa Cruz), catalase (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... RRID:AB_2072134, 1:1,000), and Cyclin D1 (sc-753, RRID:AB_2070433, 1:1,000) were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... anti-CAPN2 (sc-373966, 1:10000 for immunoblotting and 1:250 for immnostaining, Santa Cruz Biotechnology), anti-NeuN (ab177487 ...