1 Hydroxymethyl benzo cd indol 2 1H one citations for Santa Cruz

Quote: ... then soaking the gel for 2 hr with shaking in 1X TBE supplemented with 4.5 mg/mL chloroquine phosphate (Santa Cruz Biotech). The gel was then turned 90° and electrophoresed in the orthogonal direction at 130 V for 60 min in 1X TBE supplemented with 4.5 mg/mL chloroquine phosphate ...

UNC-10/SYD-2 complex is sufficient to link kinesin-3 to RAB-3 containing synaptic vesicles in the absence of the motor’s PH domain

Prerana Bhan, et al., bioRxiv - Cell Biology 2020

Quote: ... 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996, Santa Cruz). Western blotting (Fig ...

CBL mutations promote activation of PI3K/AKT signaling via LYN kinase

Roger Belizaire, et al., bioRxiv - Cancer Biology 2020

Quote: ... cells were plated in 48-well plates (2×105 cells/well in 600μL) and mixed with polybrene (Santa Cruz Biotechnology, 134220) to a final concentration of 4μg/mL and 100-200μL undiluted lentiviral supernatant ...

SUMO Modifies GβL and Mediates mTOR Signaling

Sophia Louise Lucille Park, et al., bioRxiv - Biochemistry 2020

Quote: ... GβL (sc-425272) and SUMO1/2/3 deletion were carried out in striatal cells using CRISPR/Cas-9 tools obtained from Santa Cruz, as described before [46 ...

Drug addiction mutations unveil a methylation ceiling in EZH2-mutant lymphoma

Hui Si Kwok, et al., bioRxiv - Cancer Biology 2022

Quote: ... Pfeiffer and Karpas-422 were transduced by spinfection at 1,070 x g (5 acceleration, 2 brake) for 90 min at 37 °C with 8 µg/ml polybrene (Santa Cruz Biotechnology). After 48 h post-transduction ...

Transcriptional changes in Plasmodium falciparum upon conditional knock down of mitochondrial ribosomal proteins RSM22 and L23

Swati Dass, et al., bioRxiv - Microbiology 2022

Quote: ... The membrane was blocked with 5% milk/PBS for 2 h before incubation with mouse monoclonal anti-HA antibody (Sc-7392, Santa Cruz) at 1:10,000 for overnight at 4°C ...

Inhibition of lysyl oxidase-like 2 ameliorates folic acid-induced renal tubulointerstitial fibrosis

Sung-Eun Choi, et al., bioRxiv - Pathology 2020

Quote: ... Transfected cells were selected by selection media containing 2 μg/ml puromycin dihydrochloride (cat. no. sc-108071; Santa Cruz Biotechnology, Inc.). After confirming the decrease in LOXL2 expression by reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...

Inhibition of lysyl oxidase-like 2 ameliorates folic acid-induced renal tubulointerstitial fibrosis

Sung-Eun Choi, et al., bioRxiv - Pathology 2020

Quote: ... HK-2 cells were treated with media containing 5 μg/ml of polybrene (cat. no. sc-134220; Santa Cruz Biotechnology, Inc.), and then LOXL2 shRNA lentiviral particles and control shRNA lentiviral particles of 1 and 2 multiplicity of infection (MOI ...

Intercellular telomere transfer extends T cell lifespan

Bruno Vaz, et al., bioRxiv - Immunology 2020

Quote: Human T cells (2-5 x 106) were transfected with 3 μg of TERT KO CRISPR/Cas9 plasmid (h) (sc-400316, Santa Cruz) according to the manufacturer instructions ...

The HIV-1 Vpu transmembrane domain topology and formation of a hydrophobic interface with BST-2 are critical for Vpu-mediated BST-2 downregulation

Nabab Khan, et al., bioRxiv - Cell Biology 2020

Quote: ... for EGFP-tagged Vpu/F1/F2 detection, Nef antibody (EEH1, NIH AIDS Reagent Program) and anti-HA antibody for HA-tagged BST-2 protein (Santa Cruz). Secondary antibodies conjugated to HRP (Calbiochem ...

Insulin-dependent and -independent dynamics of insulin receptor trafficking in muscle cells

Haoning Howard Cen, et al., bioRxiv - Cell Biology 2021

Quote: ... INSR was immunoprecipitated by incubating 500 μg of protein lysate with 2 μg INSR antibodies (Rabbit pAb, Cat. #sc-711, Santa Cruz) at 4°C overnight ...

Erosion of X-Chromosome Inactivation in female hiPSCs is heterogeneous and persists during differentiation

Ana Cláudia Raposo, et al., bioRxiv - Molecular Biology 2024

Quote: ... All samples and standards had a heavy isotope-labeled nucleoside mix added prior to mass spectral analysis (2′-deoxycytidine-13C1, 15N2 (#SC-214045, Santa Cruz), 5-(methyl-2H3)-2′-deoxycytidine (#SC-217100 ...

Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons

Laura Sichlinger, et al., bioRxiv - Neuroscience 2024

Quote: ... SMAD and Wnt inhibition were discontinued after D3 and the neurons were matured with daily medium changes involving 10µM N-[2S-(3,5-difluorophenyl)acetyl]-L-alanyl-2-phenyl-1,1-dimethylethyl ester-glycine (DAPT; Santa Cruz Biotechnology, SC-201315A), neurotrophic factors (brain-derived neurotrophic factor (BDNF ...

Matrix architecture and mechanics regulate myofibril organization, costamere assembly, and contractility of engineered myocardial microtissues

Samuel J. DePalma, et al., bioRxiv - Bioengineering 2023

Quote: ... iPSC-CMs were resuspended in replating media (RPMI plus B27 supplement with 2% FBS and 5µM Y-27632 (Santa Cruz Biotechnology)) and 125,000 cells were seeded per fibroTUG substrate through the top of the cell seeding mask in approximately 200 µL of media ...

Stability of Genomic Imprinting and X-Chromosome Inactivation in the Aging Brain

Samantha Mancino, et al., bioRxiv - Molecular Biology 2023

Quote: ... All samples and standards had a heavy isotope-labeled nucleoside mix added prior to mass spectral analysis (2′-deoxycytidine-13C1, 15N2 (Cat# SC-214045, Santa Cruz), 5-(methyl-2H3)-2′-deoxycytidine (Cat# SC-217100 ...

Survivin prevents the Polycomb Repressor Complex 2 from methylating Histone 3 lysine 27

Maja Jensen, et al., bioRxiv - Molecular Biology 2022

Quote: ... The pre-cleared chromatin was incubated with 2μg anti-Survivin (Tang, Ling, et al (2012) BBRC 421(2) 249-254) (10811, Santa Cruz Biotechnology), anti-H3K27ac (C15410196 ...

Nuclear speckle-localized RNAs exhibit preferential positioning and orientation

Sneha Paul, et al., bioRxiv - Biophysics 2022

Quote: Transcription induction of the transfected construct was done 24 h after transfection using 2 μg/mL doxycycline (Santa Cruz # sc-204734B) in Tet-free medium ...

FBXL4 suppresses mitophagy by restricting the accumulation of NIX and BNIP3 mitophagy receptors

Giang Thanh Nguyen-Dien, et al., bioRxiv - Cell Biology 2022

Quote: ... sc-17787), rabbit polyclonal anti-HDAC6 (sc-11420), and mouse monoclonal anti-GFP (B-2, sc-9996) were obtained from Santa Cruz Biotechnology ...

Inhibitory input directs astrocyte morphogenesis through glial GABA_BR

Yi-Ting Cheng, et al., bioRxiv - Neuroscience 2023

Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology ...

Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma

Ashwin R. Iyer, et al., bioRxiv - Cancer Biology 2023

Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1 ...

Casein Kinases 2-dependent phosphorylation of the placental ligand VAR2CSA regulates Plasmodium falciparum-infected erythrocytes cytoadhesion

Dominique Dorin-Semblat, et al., bioRxiv - Microbiology 2024

Quote: ... erythrocytes membrane fractions lysates were incubated for 2 h with 3µg of mouse IgG isotype or mouse immuno-purified anti-HuCK2α (Santa Cruz Biotechnologies). Total lysates (500µg ...

A novel interaction between the 5’ untranslated region of the Chikungunya virus genome and Musashi RNA binding protein is essential for efficient virus genome replication

Kaiwen Sun, et al., bioRxiv - Microbiology 2024

Quote: ... 1 μg envelope plasmid pMDG encoding VSV-G protein and 1.5 µg lentiviral vector plasmid encoding shRNA to MSI-2 (sc-75834-SH; Santa Cruz Biotechnology). A lentiviral vector plasmid encoding scrambled shRNA sequences with no cellular targets was used as the negative control (sc-108060 ...

Diverse plant RNAs coat Arabidopsis leaves and are distinct from apoplastic RNAs

Lucía Borniego, et al., bioRxiv - Plant Biology 2024

Quote: sRNA libraries were constructed using the RealSeq-AC kit version 2 (Realseq Biosciences, Santa Cruz, CA, USA, catalog no. 500-00048;) as per the manufacturer’s instructions ...

Dysregulated Cholesterol Metabolism with Anomalous PI3K/Akt/mTOR pathway Predicts Poor Carboplatin Response in High Grade Serous Ovarian Cancer

Elizabeth Mahapatra, et al., bioRxiv - Cancer Biology 2024

Quote: ... in primary cultured ex vivo cells as well as SKOV3 and SKOV3R using 2’,7’-dichlorofluorescein dihydroacetate (DCFH-DA; Santa Cruz) as per laboratory protocol [20] ...

PD-1 blockade exacerbates Mycobacterium tuberculosis infection in rhesus macaques

Keith D Kauffman, et al., bioRxiv - Immunology 2020

Quote: ... incubated in secondary antibody for 1 hour at room temperature (donkey-α-rabbit-HRP, Jackson ImmunoResearch #711-035-152, 1:10,000; mouse-α-actin-HRP, Santa Cruz #sc-47778, 1:10,000), washed and coated in Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...

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