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Citations for Santa Cruz :
1001 - 1050 of 1144 citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... After the blots had been incubated with a HRP-labeled mouse IgGκ light chain binding protein (1:5000, sc-516102, Santa Cruz), they were reacted with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE and immunoblotting was performed as described above with anti-BAP1 antibody (Santa Cruz: sc-28383).
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed under conditions in which only covalent interactions were preserved with anti-GFP (mouse) antibody overnight at 4°C following which Protein A agarose beads (Santa Cruz) were used to precipitate the antibody bound complex.
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed and preincubated with Protein A/B PLUS agarose beads (Cat# sc-2003, Santa Cruz Biotech, Dallas, TX, USA) for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the remaining protein fraction and kept at 4 °C for 2 hrs on a rotating apparatus followed by overnight incubation with 30 μl of the protein G plus agarose beads (#SC-2002, Santa Cruz) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The following HRP-conjugated secondary antibodies or proteins were used for development of western blots at 1:5000 – 1:10000: mouse IgGκ-binding protein (m-IgGκ BP-HRP) (sc-516102, Santa Cruz); mouse monoclonal anti-rabbit IgG-HRP (sc-2357 ...
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bioRxiv - Immunology 2021Quote: ... One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz) and incubating for 1 hour at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the detection of CDK4 proteins via a quick WB procedure using the sc-601 primary antibody from Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... was from Sigma and the HRP-conjugated anti-mouse IgG-k-chain binding protein (sc-51602) as well as the anti-goat HRP-conjugated secondary antibody (sc-2350) were from Santa Cruz Biotechnology.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] reagent ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was pre-cleared by O/N incubation with 40 μL of Protein A/G beads (sc-2003, Santa Cruz biotechnology) on a stirring wheel at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein of cleared lysate were incubated with anti-GFP polysera (1:1,000) overnight prior to addition of protein-G agarose beads (Santa Cruz Biotechnology) for an additional 2 hr ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were prepared from microdissection of 10 hippocampi of juvenile mice incubated in RIPA lysis buffer (Santa Cruz sc-24948). Active Rac1 Detection was performed using Active Rac1 Detection Kit of Cell Signalling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... for EGFP-tagged Vpu/F1/F2 detection, Nef antibody (EEH1, NIH AIDS Reagent Program) and anti-HA antibody for HA-tagged BST-2 protein (Santa Cruz). Secondary antibodies conjugated to HRP (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... INSR was immunoprecipitated by incubating 500 μg of protein lysate with 2 μg INSR antibodies (Rabbit pAb, Cat. #sc-711, Santa Cruz) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Different primary antibodies were used for the detection of interest proteins: anti-IL-1β rabbit polyclonal (1:1000, H-153, SC-7884, Santa Cruz), anti-caspase-1 (p20 ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently incubated with primary antibodies rabbit anti-Prosurfactant Protein C (pro-SPC) (MilliporeSigma, Burlington, MA, USA, #AB3786, RRID: AB_91588) and mouse anti-Acetylated α Tubulin (ACT) (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... For co-localization studies employing confocal microscopy the following antibody combinations were used: mouse anti-non-structural protein 1 (Santa Cruz)/goat anti-mouse Alexa568 (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1,000, catalog no. sc-9996, Santa Cruz Biotechnology) and anti-RFP antibody (1/2000 ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl DynabeadsTM Protein G beads were first coated with 1 µg mouse anti-MYC antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG1 (sc-3877 ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting immune complex was then incubated for 1 h with 40 µl of protein A/G plus agarose beads (sc-2003, Santa Cruz). After this ...
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bioRxiv - Cell Biology 2023Quote: ... 4 °C) and then pre-cleared with 20 μl of protein A/G PLUS agarose beads (sc-2003, Santa Cruz Biotechnology) for 1 hour at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... supernatant was incubated with anti-NLRP3 or anti-ZDHHC7 antibody as indicated and Protein A/G conjugated agarose beads (Santa Cruz) to immunoprecipitate endogenous NLRP3 or ZDHHC7 proteins ...
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bioRxiv - Neuroscience 2023Quote: ... anti-S6 ribosomal protein (S6) (mouse monoclonal clone C-8, #sc-74459, RRID: AB_1129205, Santa Cruz Biotechnology; 1:1000 for WB), anti-4E-BP1 (rabbit monoclonal clone 53H11 ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were stripped and re-probed with a polyclonal antibody directed against the immunoprecipitated GRK2 protein (C15, Santa Cruz Biotechnology Inc). Blots were developed using a chemiluminescent method (ECL ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed three-times with TBST (TBS containing 0.1% Tween-20) and incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000; Santa Cruz Biotechnology, USA) for 2 hrs at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The following are the specificities and sources of the commercially available antibodies used in the present study: antimyelin basic protein (catalog #sc-13914, Santa Cruz Biotechnology), anti-NG2 proteoglycan (catalog #AB5320 ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were added for 3 h before 45 min incubation with protein A or G beads (Dynabeads 10002D, 10003D from Thermofisher scientific, or agarose beads sc-2001, sc-2001 from Santa Cruz Biotechnology) for rabbit or mouse IgG respectively ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein, #516102, Santa Cruz), and the HRP activity was detected by incubation with ECL and exposure to a CCD camera ...
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bioRxiv - Immunology 2021Quote: ... pre-cleared with protein A agarose beads (Pierce) and incubated with agarose conjugated anti-Rad52 mAb (clone F-7; sc-365341 AC, Santa Cruz Biotechnology) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Presence of His-tagged protein was detected by chemical luminescence using His-probe (H-3) HRP (sc-8036 HRP, Santa Cruz Biotechnology) monoclonal antibody horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... samples containing same protein amount were subjected to immunoprecipitation with rabbit polyclonal antibody against PDE4B (H-56) (#sc-25812, Santa Cruz Biotechnology) coupled to GammaBind Plus Sepharose beads (# 17-0886-02 ...
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bioRxiv - Microbiology 2021Quote: ... with the lysate overnight at 4 °C followed by addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX) and incubation for 2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibody membranes were incubated with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:2500) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated in TBST containing 2% (w/v) BSA with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:5000) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cleared by incubating with 2.0 µg of the control IgG and 20 µL vortexed Protein G PLUS-Agarose beads (Santa Cruz, sc-2002) for 30 min with rocking at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates (500 μg) were pre-cleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz, catalog #: sc-2003) and 1.0 μg of normal mouse IgG antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Immunology 2019Quote: ... immunoprecipitated with 2 µg/sample of indicated antibodies overnight at 4 °C before addition of washed protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 1 hr with rotation at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 10 μl of a polyclonal ROCK1 antibody (sc-6055, Santa Cruz Biotechnology) coupled to protein G-Sepharose beads (GE healthcare ...