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Citations for Santa Cruz :
1001 - 1050 of 1223 citations for Human Gap Junction Alpha 8 Protein CX50 GJA8 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... We used the following commercial kits from Santa Cruz technology ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was precleared by incubation with Dynabeads protein G magnetic beads and anti-mouse IgG (sc-2025, Santa Cruz) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The total protein expression level of the anti-CD19 CAR was detected with an anti-CD3z antibody (Santa Cruz Biotechnology).
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bioRxiv - Cancer Biology 2021Quote: ... B-cell lymphoma-extra large (Bcl-xL) and cellular inhibitor of apoptosis protein-1 (cIAP1) were supplied by Santa Cruz Biotechnology (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was incubated for 2 h with 1.5 µg antibody and subsequently for 1 h with 30 µl protein A/G agarose beads (sc-2003, Santa Cruz), or for 3 h either with 25 µl of M2 anti-FLAG affinity gel (A2220 ...
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bioRxiv - Neuroscience 2021Quote: ... The Gαo polyclonal antibody recognizes an epitope located between positions 90-140 of the Gαo protein (Santa Cruz, personal communication).
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bioRxiv - Immunology 2021Quote: ... cell lysates were incubated with anti-Flag (M2) beads or with protein G Plus-Agarose immunoprecipitation reagent (Santa Cruz Biotechnology) together with 1 μg of the corresponding antibodies at 4°C overnight on a roller ...
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bioRxiv - Genetics 2022Quote: ... per 100 μL of the supernatant was added with 10 μL Protein A/G PLUS-Agarose (Santa Cruz, CA, USA) and incubated with the Anti-Flag antibody overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate/antibody mixture were then incubated with protein A/G-agarose beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 2-hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate/antibody mixture were then incubated with protein A/G-agarose beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 2-hour at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Protein bands detected by incubation for12-16h at 4°C with polyclonal primary antibodies to GAPDH(sc-32233, Santa Cruz), USP21(ab171028 ...
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lysates were incubated with 20 µL pre-coupled antibody-protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 3 hr at 4 °C rotating ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated in 10% SDS–PAGE gels and transferred onto a nitrocellulose membrane (Santa Cruz Biotech, Dallas, TX, USA). Membranes were blocked in 5% w/v nonfat dry milk in 1x Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Hedgehog pathway protein localization coverslips were incubated with either 1) mouse anti-SMO (1:50, Santa Cruz Biotechnology, sc-166685) and rabbit anti-ARL13B (1:800 ...
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bioRxiv - Neuroscience 2024Quote: Whole-cell protein lysates were prepared from hCO or enriched astrocytes using RIPA lysis buffer (Santa Cruz Biotechnology, sc-24948A) within HypoxyLab after 48 hr of hypoxia exposure ...
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bioRxiv - Biochemistry 2024Quote: ... and as secondary antibody 1:5000 diluted mouse IgG kappa binding protein conjugated to Horseradish Peroxidase (Santa Cruz, sc-516102). Nitrocellulose membranes (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS–polyacrylamide gel electrophoresis and detected with the following antibodies: ARF6 (Santa Cruz Biotechnology sc-7971), ASAP1 (Santa Cruz Biotechnology sc-81896) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were precleared for 1h at 4°C with the addition of Protein A/G PLUS-Agarose (Santa Cruz Biotechnologies). Chromatin was immunoprecipitated overnight at 4°C using antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS– polyacrylamide gel electrophoresis and detected with the following antibodies: Vinculin (Santa Cruz Biotechnology sc-25336), Tubulin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The protein levels of PG carboxypeptidases and DnaK were determined using monoclonal antibodies against Flag-tag (Santa Cruz Biotechnology, USA) and anti-DnaK (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Bead-based immunoprecipitation was performed with protein G Dynabeads and IgG anti-BrdU antibody IIB5 (Santa Cruz Biotechnology, SC-32323). RNA was extracted and RT-qPCR performed as previously described.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Biochemistry 2022Quote: ... and the expression of both G3BP1-FRB and FKBP-hADAR(E488Q) proteins was confirmed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Microbiology 2022Quote: ... Total MAPK proteins were detected using a commercial mouse monoclonal anti-Mpk1 antibody (sc-165979, Santa Cruz Biotechnology, Heidelberg, Germany), a custom-designed polyclonal anti-Fus3/anti-Fmk1 antibody (SICGEN Research and Development in Biotechnology Ltd ...
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bioRxiv - Molecular Biology 2023Quote: ... as a primary and mouse IgG κ-binding protein horseradish peroxidase (1:50,000 dilution; Santa Cruz Biotechnology, Dallas, United States) as a secondary antibody ...
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bioRxiv - Physiology 2024Quote: ... 50 μg of total protein extraction was used for semiquantitative analysis of CLDN5 (Santa Cruz Biotechnology, sc-374221, TX, USA). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... and a 1:100 dilution of a mouse monoclonal antibody to the ribosomal protein L4 (Sc-100838 Santa Cruz Biotechnology) in a blocking solution at 4° C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Each hippocampus was weighed and transferred into 500 μl of ice-cold lysis buffer (NPER, Neuronal Protein Extraction Reagent, ThermoScientific) containing sodium orthovanadate (0.5 mM, Santa Cruz), phenyl-methylsulfonyl fluoride (PMSF ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated for 1 hour with a Yes-associated protein (YAP) primary antibody (Santa Cruz Biotechnology, sc101199, 1:200) followed by secondary antibody labeling ...
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bioRxiv - Biochemistry 2024Quote: ... Pan anti-Kbhb antibody was first conjugated to Protein A/G plus agarose (Santa Cruz Biotechnology Inc, Cat# sc-2003) and then incubated with tryptically digested peptides with gentle agitation overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... PVDF membranes with transferred proteins were incubated with the anti-Tau primary antibodies: 0.2 μg/mL Tau13 (sc-21796 AF680, Santa Cruz) or 0.2 μg/mL AT8 (ab64193 ...
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bioRxiv - Cancer Biology 2021Quote: ... Separated proteins were transferred to nitrocellulose membranes and immunoblotted with primary antibodies specific for PAK3 (Santa Cruz sc-1871, 1:200), c-Jun (CST #9165 ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL of magnetic Protein G Dynabeads were coated with 4 g of anti-GATA4 (G4) antibody (sc-25310X, Santa Cruz) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies specific for the following proteins were obtained from the indicated suppliers: CD81 (1:1,000, Santa Cruz Biotechnology, Dallas, TX, USA), CD9 (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... 3 μg each) or isotype control antibodies (rabbit IgG or mouse IgG, respectively) were coupled to protein A/G agarose beads (Santa Cruz) in coupling buffer (0.01M sodium phosphate ...
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bioRxiv - Cancer Biology 2022Quote: ... After centrifugation at 13,200 rpm for 20 minutes at 4°C a pre-clearing step was performed by adding 25 µl of Protein A/G PLUS-Agarose beads (Santa Cruz) and incubating the samples for 1 hour at 4°C in rotation ...
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bioRxiv - Immunology 2021Quote: ... total protein was extracted by lysing tissue in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (Santa Cruz #sc-24948), sodium orthovanadate ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996, Santa Cruz). Western blotting (Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... The co-IP was perform by incubating extract with protein A or G Dynabeads conjugated with antibodies against PRDM9 (custom-made) or EWSR1 (sc-6532, Santa Cruz) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and lysates were precleared with salmon sperm DNA/protein A agarose slurry for 1h and incubated with rabbit IgG (Santa Cruz) or SREBP1 antibody in the presence of protein A agarose beads overnight ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were transferred into new tubes after centrifugation for 10 min at 14,000g and further incubated with the indicated antibodies for 3 hours at 4 □ followed by the addition of protein A/G beads (Santa Cruz), or with Anti-Flag magnetic beads (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by the addition of protein A/G agarose beads (20 μL per sample; Santa Cruz Biotechnology, sc-2003) and incubation overnight at 4 °C with rocking ...
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bioRxiv - Plant Biology 2020Quote: ... TPR2-myc protein expression was verified by western blot using c-Myc antibody sc-789 (Santa Cruz Biotechnology, Dallas, TX, USA).
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bioRxiv - Physiology 2020Quote: ... After the blots had been incubated with a HRP-labeled mouse IgGκ light chain binding protein (1:5000, sc-516102, Santa Cruz), they were reacted with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE and immunoblotting was performed as described above with anti-BAP1 antibody (Santa Cruz: sc-28383).
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed under conditions in which only covalent interactions were preserved with anti-GFP (mouse) antibody overnight at 4°C following which Protein A agarose beads (Santa Cruz) were used to precipitate the antibody bound complex.
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed and preincubated with Protein A/B PLUS agarose beads (Cat# sc-2003, Santa Cruz Biotech, Dallas, TX, USA) for 2 h ...