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Citations for Santa Cruz :
951 - 1000 of 1038 citations for Recombinant Human REG4 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were pre-cleared with control IgG for 1 hr at 4 °C and Protein A/G Plus-Agarose beads (Santa Cruz Biotechnology) for 30 min at 4 °C before incubation with primary antibody overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cbl was immunoprecipitated from 20-50 mg of total protein from each treatment using anti-Cbl AB coupled to agarose beads (sc-170 AC, Santa Cruz Biotechnology). The immunoprecipitated proteins were eluted from Cbl AB-agarose beads with Pierce IgG Elution Buffer (21004 ...
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bioRxiv - Immunology 2022Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Cancer Biology 2022Quote: ... samples were immunoprecipitated using monoclonal mouse anti-WWOX cocktail antibodies53 together with protein A/G plus agarose beads (Santa Cruz, Texas, USA) and incubated overnight at 4°C on a rotor ...
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bioRxiv - Genomics 2023Quote: ... Samples were pre-cleaned by incubating with 2–4 μg of normal immunoglobulin G (IgG) and 50 μl of A / G-agarose protein microspheres (Santa Cruz Biotechnology) for 1 hour at 4 °C with agitation for preclearance ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately 750 µg of solubilized proteins were incubated with 1 µg of anti-C/EBPβ mouse IgG (Santa Cruz Biotech, sc-7962) or normal mouse IgG (Santa Cruz Biotech ...
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bioRxiv - Immunology 2023Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Precleared extracts were incubated with 40 μL of 50% protein A/G agarose and 5 of normal rabbit IgG (Santa Cruz Biotech), H3K27ac (Active Motif ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to HRP (1:2000) (sc-516102, Santa Cruz Biotechnology) for 3h at 4°C on a rotary and washed thrice with TBST for 10 min each time ...
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bioRxiv - Microbiology 2024Quote: ... the formation of a fusion protein with a proper size was validated by Western blotting using anti-myc antibody 9E10 (Santa Cruz Biotechnology), and anti-Xpress tag antibody R91025 (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation at 13,200 g for 15 min at 4°C, equal amounts of cell lysates were preincubated with mouse IgG (Applygene, C2118) and Protein A/G agarose beads (Santa Cruz, sc-2003) for 4 h at 4°C and then clarified by centrifugation ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... A fraction of the mixture of protein-DNA complex was used as “input DNA.” Soluble chromatin was immunoprecipitated with anti-VDRE antibody (D-6, Santa Cruz Biotechnology Inc) and normal mouse immunoglobulin G (IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Developmental Biology 2022Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels with the following antibodies anti-NFATc1 (1:1000, clone 7A6, Santa Cruz) and anti-GAPDH (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... A precleaning step of the samples was performed by adding 50 μl of Protein G-Plus agarose beads suspension (Santa Cruz Biotechnology, INC) to 1 ml of each sample (Chimera ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and identified by immunoblotting with a mouse anti-green fluorescent protein (GFP) (Santa Cruz Biotechnology, Santa Cruz, CA), or anti-Atg13 [175] antibody to detect tagged or endogenous proteins ...
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in RIPA buffer and 100 μg of total protein were immunoprecipitated with an antibody recognizing total Stat92E (Santa Cruz, sc-15708) at 4 °C overnight ...
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bioRxiv - Biochemistry 2019Quote: ... When blotting for total proteins (Cell Signaling Technology; EGFR #4267, p44/42 MAPK #4695, β-Actin #3700 and Santa Cruz Biotechnology; PTPRJ #376794), the membranes were blocked with 5% dry milk in TBS-T for 1 h at RT ...
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bioRxiv - Immunology 2019Quote: ... The immunoreactive proteins were visualized with horseradish peroxidase (HRP)-conjugated secondary antibody (polyclonal goat anti-rabbit IgG, Santa Cruz Biotechnology, cat.no. sc-2004) and SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: Coimmunoprecipitation was performed as previously described (Waxman, Baconguis, Lynch, & Robinson, 2007) with the following substitutions: Protein A/G agarose (Santa Cruz Biotechnology, Santa Cruz, CA) was used to bind 3ug of antibody ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653) in the dark at room temperature for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Nucleus proteins of the ANKRD49-OE or LV5 H1299 cells (1×106) were extracted and 25 µg of them were precleared using Protein A/G Agarose (Santa Cruz, California, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining supernatant was subjected to immunoprecipitation using 15 μl of mouse monoclonal antibody against green fluorescent protein (catalog no. sc-9996, Santa Cruz, CA, USA). The immunoprecipitate was gently shaken overnight at 4 °C ...