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• CDK12 Loss Promotes Prostate Cancer Development While Exposing Vulnerabilities to Paralog-Based Synthetic Lethality

  Jean Ching-Yi Tien, et al., bioRxiv - Cancer Biology 2024

  Quote: ... α-TUBULIN (Santa Cruz 3873S), RNA polymerase II subunit B1 phosphor CTD Ser-2 Antibody ...
• Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosis

  Daisuke Ito, et al., bioRxiv - Neuroscience 2024

  Quote: ... Membranes were reprobed with an anti-GAPDH antibody (MAB374, 1:5000; Santa Cruz Biotechnology) for normalization.
• Neuroprotection of low dose carbon monoxide in Parkinson’s disease models commensurate with the reduced risk of Parkinson’s among smokers

  KN Rose, et al., bioRxiv - Neuroscience 2024

  Quote: ... sections were incubated with anti-HuD directly conjugated to Alexa Fluor 647 (Santa Cruz cat# sc-28299, 1:50) for 1 hour at room temperature ...
• Infection of human organoids supports an intestinal niche for Chlamydia trachomatis

  Pargev Hovhannisyan, et al., bioRxiv - Microbiology 2024

  Quote: ... Following primary antibodies were used: anti-HSP60 (Santa Cruz, sc-57840), anti-E-cadherin (Becton Dickinson ...
• A platform for mapping reactive cysteines within the immunopeptidome

  Chenlu Zhang, et al., bioRxiv - Systems Biology 2024

  Quote: ... The anti-β-Actin antibody (clone#: C4, cat#: sc-47778) was purchased from Santa Cruz Biotechnology ...
• Three Modes of Viral Adaption by the Heart

  Cameron D. Griffiths, et al., bioRxiv - Systems Biology 2024

  Quote: ... p38 (Santa Cruz Biotechnology, sc-535, 1:5000 dilution); p38 p-Thr180/Tyr182 (Cell Signaling Technology ...
• The regulatory architecture of the primed pluripotent cell state

  Bo I. Li, et al., bioRxiv - Systems Biology 2024

  Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and immunostained for Oct4 (Pou5f1) (Santa Cruz sc- 5279, 1:1,000) using an Alexa Fluor 488 secondary antibody (Invitrogen A11029 ...
• Quantitative modelling of signaling in aggressive B cell lymphoma unveils conserved core network

  Bertram Klinger, et al., bioRxiv - Systems Biology 2024

  Quote: ... and α-mouse HRP polyclonal goat (D1609) and α-rabbit HRP polyclonal goat (E1710) (all from Santa Cruz Biotechnology, Inc.).
• Elucidation and Pharmacologic Targeting of Master Regulator Dependencies in Coexisting Diffuse Midline Glioma Subpopulations

  Ester Calvo Fernández, et al., bioRxiv - Systems Biology 2024

  Quote: ... Virus in the supernatant was precipitated with PEG-6000 (Santa Cruz Biotechnology sc-302016), suspended in appropriate media and pooled ...
• Optogenetic Regulation of EphA1 RTK Activation and Signaling

  Anna I Wurz, Kevin Zheng, Robert M. Hughes, bioRxiv - Synthetic Biology 2024

  Quote: ... Blots were then incubated with primary antibody in a 1:1000 dilution with 5% BSA in TBST overnight at 4°C: tyrosine phosphorylation using phosphotyrosine mouse monoclonal Ig pY99 (Santa Cruz Biotechnologies); mCherry expression using anti-mCherry rabbit polyclonal Ig (Rockland Inc.) ...
• Epitranscriptomic Reader YTHDF2 Regulates SEK1(MAP2K4)-JNK-cJUN Inflammatory Signaling in Astrocytes during Neurotoxic Stress

  Emir Malovic, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse cJUN (Santa Cruz, Dallas, TX: #sc-74543), rabbit pJNK (CST ...
• A functional interaction between TDP-43 and USP10 reveals USP10 dysfunction in TDP-43 proteinopathies

  A Marrero-Gagliardi, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti p53 (WB 1:500, Santa Cruz Biotechnology, sc-126); mouse anti Tubulin (WB 1:10000 ...
• A functional interaction between TDP-43 and USP10 reveals USP10 dysfunction in TDP-43 proteinopathies

  A Marrero-Gagliardi, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti GAPDH (WB 1:250, Santa Cruz Biotechnology, sc-32233); anti mouse Alexa Fluor 488 (IF secondary 1:600 ...
• Increased Cholesterol Synthesis Drives Neurotoxicity in Patient Stem Cell-Derived Model of Multiple Sclerosis

  Rosana-Bristena Ionescu, et al., bioRxiv - Neuroscience 2024

  Quote: ... Oct4 (Santa Cruz, 1:200), SOX17 (R&D ...
• PP2B-dependent cerebellar plasticity sets the amplitude of an innate reflex during juvenile development

  Bin Wu, et al., bioRxiv - Neuroscience 2024

  Quote: ... Dyn1 (1:1000, mouse monoclonal, Santa cruz), and probed with secondary antibodies subsequently ...
• A complex mechanism translating variation of a simple genetic architecture into alternative life-histories

  Jukka-Pekka Verta, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... we used mouse anti-rabbit IgG-HRP sc-2357 (Santa Cruz Biotechnology, Texas, USA) and Precision Protein StrepTactin-HRP Conjugate (for protein standards ...
• Structural basis of Cdk7 activation by dual T-loop phosphorylation

  Robert Düster, et al., bioRxiv - Biochemistry 2024

  Quote: ... 1:1000 (Santa Cruz #sc-7344); α-phospho Cdk7 (pT170) ...
• FAK and p130Cas modulate stiffness-mediated early transcription and cellular metabolism

  Bat-Ider Tumenbayar, et al., bioRxiv - Genomics 2024

  Quote: ... or GAPDH (cat. no. sc-25778, Santa Cruz Biotechnology). Immunoblot signals were detected using enhanced chemiluminescence.
• Microvilli regulate the release modes of alpha-tectorin to organize the domain-specific matrix architecture of the tectorial membrane

  Ava Niazi, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
• Proteome-scale tagging and functional screening in mammalian cells by ORFtag

  Filip Nemčko, et al., bioRxiv - Systems Biology 2024

  Quote: ... Reporter cells were then transduced with the virus in the presence of 8 µg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). After 7 days of transduction ...
• MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

  David Molina, et al., bioRxiv - Plant Biology 2024

  Quote: ... Fluorol Yellow (Santa Cruz Cat# sc-215052) was employed ...
• XopM, a FFAT motif containing type-III effector protein from Xanthomonas, suppresses PTI responses at the plant plasma membrane

  Charlotte Brinkmann, et al., bioRxiv - Plant Biology 2024

  Quote: ... GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000; Santa Cruz Biotechnology Inc. ...
• Distinct human stem cell subpopulations drive adipogenesis and fibrosis in musculoskeletal injury

  Steven M. Garcia, et al., bioRxiv - Cell Biology 2024

  Quote: ... Primary antibodies and dilutions used for stains of cultured cells: anti-CD10 1:100 (Santa Cruz Biotechnology, INC., #sc-19993) and anti-CD55 1:100 (Invitrogen ...
• Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice

  Farooq Syed, et al., bioRxiv - Physiology 2024

  Quote: ... mouse anti-CD68 (Santa Cruz), and 1:20,000 Syto83 (Invitrogen ...
• Impact of Mesotherapy with Sodium Deoxycholate on Liver: Metabolic- and Sex-Specific Insights in Swiss mice

  Leidyanne Ferreira Gonçalves, et al., bioRxiv - Pathology 2024

  Quote: ... and anti-β-actin (internal control, 1:5,000, mouse, sc-2005, Santa Cruz). The following day ...
• A Vector System Encoding Histone H3 Mutants Facilitates Manipulations of the Neuronal Epigenome

  Sophie Warren, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-SATB2 (Santa Cruz, Sc-81376, 1:200). To detect primary antibodies ...
• MXRA8 promotes adipose tissue whitening to drive obesity

  Wentong Jia, et al., bioRxiv - Physiology 2024

  Quote: ... and liver tissues were immediately immersed in 4% paraformaldehyde (PFA) in PBS (cat# sc-281692, Santa Cruz Biotechnology, Dallas, TX) and kept at 4°C for at least 3 days while protected from light ...
• Impaired transitioning of the FXR ligand binding domain to an active state underlies a PFIC5 phenotype

  Annika Behrendt, et al., bioRxiv - Physiology 2024

  Quote: ... cells were stained for 1h at 1:100 with rabbit anti-FXR (H-130; sc-13063, Santa Cruz Biotechnology) followed by staining at 1:250 with goat anti-rabbit-IgG-FITC (Jackson ImmunoResearch ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... MCU (1:1000, sc-515930, Santa Cruz) and VDAC1 (1:500 ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... membranes were washed with Tris-buffered saline containing 0.1% Tween-20 and then incubated with anti-rabbit IgG secondary antibody coupled to HRP (1:10000, sc-2357, Santa Cruz) for 1 h or an anti-mouse (1:5000 ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... Sigma-Aldrich),1 µM dexamethasone (SC-29059, Santa Cruz) and100 nM insulin (Actrapid ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... and VDAC1 (1:500, sc-390996, Santa Cruz). Antibodies were used according to the manufacturer’s instructions ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... Vasopressin (1nM) (sc-356188, Santa Cruz), Glucagon (100nM) ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... The membranes were blocked with 5% nonfat dry milk for 1 h and then incubated with the following antibodies: NCLX (1:1000, sc-161921, Santa Cruz), MCU (1:1000 ...
• NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis

  Mahmoud Taha, et al., bioRxiv - Physiology 2024

  Quote: ... for 1 h or an anti-mouse (1:5000, sc-2005, Santa Cruz) solution for 1 h ...
• A bacterial type III effector hijacks plant ubiquitin proteases to evade degradation

  Wenjia Yu, et al., bioRxiv - Plant Biology 2024

  Quote: ... anti-ubiquitination(P4D1) (Santa Cruz Blotechnology sc-8017 HRP), anti-FLAG (Abmart ...
• Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development in Arabidopsis thaliana

  Qian Ma, et al., bioRxiv - Plant Biology 2024

  Quote: ... and goat anti-mouse (Santa Cruz sc-2005). Blots were developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific 34075 ...
• Connexin43 in mesenchymal lineage cells regulates body adiposity and energy metabolism in mice

  Seung-Yon Lee, et al., bioRxiv - Physiology 2024

  Quote: ... Cx45 (Santa Cruz Biotechnology, sc-374354), Cx40 (Santa Cruz Biotechnology ...
• Connexin43 in mesenchymal lineage cells regulates body adiposity and energy metabolism in mice

  Seung-Yon Lee, et al., bioRxiv - Physiology 2024

  Quote: ... Cx40 (Santa Cruz Biotechnology, sc-365107), or β-actin (Cell signaling ...
• Connexin43 in mesenchymal lineage cells regulates body adiposity and energy metabolism in mice

  Seung-Yon Lee, et al., bioRxiv - Physiology 2024

  Quote: ... and incubated overnight with primary antibody against mouse Cx43 (unconjugated F-7, mouse, Santa Cruz Biotechnology) 1/200 in PBS/0.1% Triton™ X-100 (Sigma-Aldrich ...
• Cardiac Localized Polycystin-2 plays a Functional Role in Natriuretic Peptide Production and its Absence Contributes to Hypertension

  Brandon Elliott, et al., bioRxiv - Physiology 2024

  Quote: ... B-type natriuretic peptide (1:100; Santa Cruz), polycystin 2 (1:100 ...
• Cardiac Localized Polycystin-2 plays a Functional Role in Natriuretic Peptide Production and its Absence Contributes to Hypertension

  Brandon Elliott, et al., bioRxiv - Physiology 2024

  Quote: ... atrial natriuretic peptide (1:100; Santa Cruz), B-type natriuretic peptide (1:100 ...
• Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis

  Arivarasan Sampathkumar, et al., bioRxiv - Genetics 2024

  Quote: ... anti-Cdc5 (sc-33625, Santa Cruz), and histone H3 phospho-T11 (ab5168 ...
• Crosstalk interactions between transcription factors ERRα and PPARα assist PPARα-mediated gene expression

  Sofie J. Desmet, et al., bioRxiv - Molecular Biology 2024

  Quote: ... After fixation, endogenous PPARα was visualized using mouse anti-PPARα (H-2, sc-398394) antibody (Santa Cruz Biotechnology), followed by Alexa Fluor 568 anti-mouse IgG (Molecular Probes ...
• Cbfβ regulates Wnt/β-catenin, Hippo/Yap, and TGFβ signaling pathways in articular cartilage homeostasis and protects from ACLT surgery-induced osteoarthritis

  Wei Chen, et al., bioRxiv - Pathology 2024

  Quote: ... and rabbit anti-mouse IgG-HRP (Santa Cruz Biotechnology Cat# sc-358917 ...
• Cbfβ regulates Wnt/β-catenin, Hippo/Yap, and TGFβ signaling pathways in articular cartilage homeostasis and protects from ACLT surgery-induced osteoarthritis

  Wei Chen, et al., bioRxiv - Pathology 2024

  Quote: ... Secondary antibodies were goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology Cat# sc-2004 ...
• Different outcomes of endurance and resistance exercise in skeletal muscles of Oculopharyngeal muscular dystrophy

  Alexis Boulinguiez, et al., bioRxiv - Pathology 2024

  Quote: ... and phosphatase inhibitor cocktail (Santa Cruz; sc-45064)] ...
• Generation and characterization of CRISPR-Cas9-Mediated XPC Gene Knockout in Human Skin Cells

  Ali Nasrallah, et al., bioRxiv - Genetics 2024

  Quote: ... excluding the step of 2M HCL incubation and by using 1/200 primary antibody targeting XPC (mouse, Santa Cruz, sc-74410 or vimentin (rabbit ...
• Silybin A from Silybum marianum reprograms lipid metabolism to induce a cell fate-dependent class switch from triglycerides to phospholipids

  Solveigh C. Koeberle, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... Mouse anti-GRP78/BiP (A-10, #sc-376768) was purchased from Santa Cruz Biotechnology (Dallas ...
• Extracellular electrophysiology on clonal human β-cell spheroids

  Emilie Puginier, et al., bioRxiv - Cell Biology 2024

  Quote: ... polyclonal goat anti-somatostatin (Santa Cruz, sc-7819), monoclonal anti-GFP ...