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Citations for Santa Cruz :
901 - 950 of 1099 citations for Recombinant Human GALNT1 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The protein complexes were analyzed by SDS/PAGE followed by WB analysis with anti-GST (B14; #sc-138; Santa Cruz) and anti-His (#R940-25 ...
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bioRxiv - Molecular Biology 2021Quote: ... The total protein expression level of the anti-CD19 CAR was detected with an anti-CD3z antibody (Santa Cruz Biotechnology).
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bioRxiv - Molecular Biology 2019Quote: ... pre-cleared samples were incubated with 20μL of protein A/G plus-agarose beads and 11μL of myc-tag antibody (9E10) (Santa Cruz Biotechnology) and rotated overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos of the total protein lysates were used for western blot analysis using anti-Cdk1 antibody (1:300, Santa Cruz) and anti-beta-Actin antibody (1:1000 ...
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Induction of p73, Δ133p53, Δ160p53, pAKT leads to neuroprotection via DNA repair by 5-LOX inhibitionbioRxiv - Neuroscience 2019Quote: ... Membrane was blocked in 5%NFM and then incubated with primary antibodies of following proteins: p53 (1:500, Santa Cruz), Akt p-Akt ...
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bioRxiv - Cancer Biology 2021Quote: ... B-cell lymphoma-extra large (Bcl-xL) and cellular inhibitor of apoptosis protein-1 (cIAP1) were supplied by Santa Cruz Biotechnology (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was incubated for 2 h with 1.5 µg antibody and subsequently for 1 h with 30 µl protein A/G agarose beads (sc-2003, Santa Cruz), or for 3 h either with 25 µl of M2 anti-FLAG affinity gel (A2220 ...
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bioRxiv - Neuroscience 2021Quote: ... The Gαo polyclonal antibody recognizes an epitope located between positions 90-140 of the Gαo protein (Santa Cruz, personal communication).
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bioRxiv - Immunology 2021Quote: ... cell lysates were incubated with anti-Flag (M2) beads or with protein G Plus-Agarose immunoprecipitation reagent (Santa Cruz Biotechnology) together with 1 μg of the corresponding antibodies at 4°C overnight on a roller ...
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bioRxiv - Genetics 2022Quote: ... per 100 μL of the supernatant was added with 10 μL Protein A/G PLUS-Agarose (Santa Cruz, CA, USA) and incubated with the Anti-Flag antibody overnight at 4 °C ...
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bioRxiv - Physiology 2019Quote: ... cell lysates were mixed with various antibodies and precipitated with protein A/G agarose beads (Santa Cruz Biotechnology, Dallas, TX) or FLAG M2 monoclonal antibody affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate/antibody mixture were then incubated with protein A/G-agarose beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 2-hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate/antibody mixture were then incubated with protein A/G-agarose beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 2-hour at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates were subjected to SDS/PAGE and immunoblotted with antibodies against AR (441, Santa Cruz Biotechnology; cat # sc-7305) or MYC tag (Cell Signaling Technology ...
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bioRxiv - Immunology 2019Quote: ... Samples were then rocked for two hours at 4° C in presence of 20 μl protein G– agarose (Santa Cruz). The beads were harvested by centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Protein bands detected by incubation for12-16h at 4°C with polyclonal primary antibodies to GAPDH(sc-32233, Santa Cruz), USP21(ab171028 ...
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lysates were incubated with 20 µL pre-coupled antibody-protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 3 hr at 4 °C rotating ...
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bioRxiv - Microbiology 2022Quote: ... The protein levels of PG carboxypeptidases and DnaK were determined using monoclonal antibodies against Flag-tag (Santa Cruz Biotechnology, USA) and anti-DnaK (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Bead-based immunoprecipitation was performed with protein G Dynabeads and IgG anti-BrdU antibody IIB5 (Santa Cruz Biotechnology, SC-32323). RNA was extracted and RT-qPCR performed as previously described.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Biochemistry 2022Quote: ... and the expression of both G3BP1-FRB and FKBP-hADAR(E488Q) proteins was confirmed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Microbiology 2022Quote: ... Total MAPK proteins were detected using a commercial mouse monoclonal anti-Mpk1 antibody (sc-165979, Santa Cruz Biotechnology, Heidelberg, Germany), a custom-designed polyclonal anti-Fus3/anti-Fmk1 antibody (SICGEN Research and Development in Biotechnology Ltd ...
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bioRxiv - Molecular Biology 2022Quote: ... Hedgehog pathway protein localization coverslips were incubated with either 1) mouse anti-SMO (1:50, Santa Cruz Biotechnology, sc-166685) and rabbit anti-ARL13B (1:800 ...
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bioRxiv - Neuroscience 2024Quote: Whole-cell protein lysates were prepared from hCO or enriched astrocytes using RIPA lysis buffer (Santa Cruz Biotechnology, sc-24948A) within HypoxyLab after 48 hr of hypoxia exposure ...
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bioRxiv - Biochemistry 2024Quote: ... and as secondary antibody 1:5000 diluted mouse IgG kappa binding protein conjugated to Horseradish Peroxidase (Santa Cruz, sc-516102). Nitrocellulose membranes (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... as a primary and mouse IgG κ-binding protein horseradish peroxidase (1:50,000 dilution; Santa Cruz Biotechnology, Dallas, United States) as a secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS–polyacrylamide gel electrophoresis and detected with the following antibodies: ARF6 (Santa Cruz Biotechnology sc-7971), ASAP1 (Santa Cruz Biotechnology sc-81896) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS– polyacrylamide gel electrophoresis and detected with the following antibodies: Vinculin (Santa Cruz Biotechnology sc-25336), Tubulin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were precleared for 1h at 4°C with the addition of Protein A/G PLUS-Agarose (Santa Cruz Biotechnologies). Chromatin was immunoprecipitated overnight at 4°C using antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated in 10% SDS–PAGE gels and transferred onto a nitrocellulose membrane (Santa Cruz Biotech, Dallas, TX, USA). Membranes were blocked in 5% w/v nonfat dry milk in 1x Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2024Quote: ... 50 μg of total protein extraction was used for semiquantitative analysis of CLDN5 (Santa Cruz Biotechnology, sc-374221, TX, USA). Then ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated for 1 hour with a Yes-associated protein (YAP) primary antibody (Santa Cruz Biotechnology, sc101199, 1:200) followed by secondary antibody labeling ...
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bioRxiv - Cancer Biology 2021Quote: ... Separated proteins were transferred to nitrocellulose membranes and immunoblotted with primary antibodies specific for PAK3 (Santa Cruz sc-1871, 1:200), c-Jun (CST #9165 ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL of magnetic Protein G Dynabeads were coated with 4 g of anti-GATA4 (G4) antibody (sc-25310X, Santa Cruz) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies specific for the following proteins were obtained from the indicated suppliers: CD81 (1:1,000, Santa Cruz Biotechnology, Dallas, TX, USA), CD9 (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... 3 μg each) or isotype control antibodies (rabbit IgG or mouse IgG, respectively) were coupled to protein A/G agarose beads (Santa Cruz) in coupling buffer (0.01M sodium phosphate ...
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bioRxiv - Cancer Biology 2022Quote: ... After centrifugation at 13,200 rpm for 20 minutes at 4°C a pre-clearing step was performed by adding 25 µl of Protein A/G PLUS-Agarose beads (Santa Cruz) and incubating the samples for 1 hour at 4°C in rotation ...
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bioRxiv - Immunology 2021Quote: ... total protein was extracted by lysing tissue in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (Santa Cruz #sc-24948), sodium orthovanadate ...
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bioRxiv - Neuroscience 2019Quote: ... and a 1 in 4 series were processed for Fos protein labelling (1:5000, rabbit polyclonal. Santa Cruz Biotechnology, CA, USA) as previously described 50 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996, Santa Cruz). Western blotting (Fig ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... The samples were precleared with Protein-G agarose beads and immunoprecipitated using antibodies against ARID1a (sc32761-x, Santa Cruz, California, USA), H3K4me3 (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in the Pierce™ IP Lysis Buffer purchased from Thermo Fisher Scientific and 1 mg of protein was allowed to bind overnight in 4°C to agarose-conjugated antibodies (Santa Cruz Biotechnology): ITGB4 (Cat # ...
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bioRxiv - Developmental Biology 2020Quote: ... The co-IP was perform by incubating extract with protein A or G Dynabeads conjugated with antibodies against PRDM9 (custom-made) or EWSR1 (sc-6532, Santa Cruz) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and lysates were precleared with salmon sperm DNA/protein A agarose slurry for 1h and incubated with rabbit IgG (Santa Cruz) or SREBP1 antibody in the presence of protein A agarose beads overnight ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were transferred into new tubes after centrifugation for 10 min at 14,000g and further incubated with the indicated antibodies for 3 hours at 4 □ followed by the addition of protein A/G beads (Santa Cruz), or with Anti-Flag magnetic beads (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by the addition of protein A/G agarose beads (20 μL per sample; Santa Cruz Biotechnology, sc-2003) and incubation overnight at 4 °C with rocking ...
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bioRxiv - Plant Biology 2020Quote: ... TPR2-myc protein expression was verified by western blot using c-Myc antibody sc-789 (Santa Cruz Biotechnology, Dallas, TX, USA).
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bioRxiv - Physiology 2020Quote: ... After the blots had been incubated with a HRP-labeled mouse IgGκ light chain binding protein (1:5000, sc-516102, Santa Cruz), they were reacted with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE and immunoblotting was performed as described above with anti-BAP1 antibody (Santa Cruz: sc-28383).