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Citations for Santa Cruz :
901 - 950 of 1020 citations for Nnn Unlabeled 2 Mg Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... USA).[105,106] Antibodies were added in Antibody Diluent: 1:50 (4 µg/mL) D-8 anti-BCL6 (Santa Cruz, USA); 1:150 (6.7 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then infected with the virus in the presence of 5 ug/ml Polybrene (Santa Cruz Biotechnology, #134220). All sequences were verified using Sanger sequencing (Laragen ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in growth media and added to mESCs along with Polybrene (4 μg/ml; Santa Cruz Biotechnology, Dallas, TX). 96 h after transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... Target cells (NIH-3T3) were infected with these retroviruses in the presence of 8μg/mL polybrene (Santa Cruz Biotechnology). After 2 days of infection ...
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bioRxiv - Biophysics 2023Quote: BioID lysis buffer was completed with 0.1% SDS and 80 U/ml benzonase nuclease (Santa Cruz Biotechnology, Dallas, TX), and lysis was followed by three cycles of water bath sonication (3□min ...
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bioRxiv - Biochemistry 2023Quote: ... Target cells were infected at limiting dilutions in the presence of 4 μg/mL of polybrene (Santa Cruz Biotechnology).
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bioRxiv - Cancer Biology 2024Quote: ... detail sequence information of each shRNA was shown in the Supplemental Table S1) were added into the culture medium with 5 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA). Transfection was discontinued by changing the culture medium 24 hours post treatment ...
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bioRxiv - Biophysics 2021Quote: ... The primary antibodies used for the immunostaining were mouse monoclonal antibodies (Arp3 [A-1], sc-48344; formin 2 [C-3], sc-376787; Santa Cruz Biotechnology, Dallas, Texas, USA). The primary antibodies were diluted 1:200 in 3% BSA and left in contact with the cells for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min at RT and incubated with the following primary antibodies at 4°C overnight in TBST with 5% BSA: monoclonal mouse GFP antibody (B-2: sc-9996, Santa Cruz Biotechnology, Inc., 1:500), polyclonal pan-XIRP2 antibody (custom generated (see above) ...
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bioRxiv - Cancer Biology 2019Quote: ... The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA) and Bcl-2 (mouse monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were incubated with Lamp1anti-goat primary antibody (1:250, sc-8099, Santa Cruz Bio-technology; Santa Cruz, CA, USA; 2 h, 4 °C) in the blocking buffer for 45 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Genomics 2021Quote: ... iPSC-derived MSCs were cultured to at least passage 6 and seeded at 4.2 × 104 cell/cm2 in culture dishes (4-10cm diameter) coated in 5ug/cm2 Type I Collagen (sc-136157, Santa Cruz Biotechnology, Dallas, TX, USA). After culturing cells for 1 day in MSC medium ...
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bioRxiv - Biophysics 2020Quote: ... A mixture of 0.5 µm Alexa Fluor 488 lysine labeled NSARS-CoV-2 and 1:100 Alexa Fluor 594 conjugated G3BP1 antibody (Santa Cruz Biotechnology, sc-365338 AF594) in cell permeabilization buffer was added to the cells and movies were recorded with 512 x 512 pixel resolution at 1000 Hz speed and 1 s per frame for about 2-3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated for 1h at room temperature in blocking buffer (5% nonfat milk in PBS pH 7.4 containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 h at room temperature with antibodies raised against insulin (Santa Cruz Biotechnology Inc, Santa Cruz, CA), the porosome associated protein ATP2C1 and actin (Santa Cruz Biotechnology Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/10000) (Zhu et al. 2010), anti-Red1 (chicken, 1/3000) (Shinohara et al. 2008) and anti-α-tubulin (YL1/2, Santa Cruz Biotechnology, 1/3000). Tubulin signals were visualized using the LI-COR Bioscience system or alkaline phosphatase stain (BCIP-NBT solution kit ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used in the indicated dilutions: anti-α-tubulin (1:1000, B-5-1-2, Santa Cruz, USA, cat no sc-23948), anti-β-actin (1:10000 ...
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bioRxiv - Biochemistry 2019Quote: ... The primary antibody used to detect UCP2 was 1:2000 dilution of anti-UCP1/2/3 raised in rabbit (Santa Cruz Biotechnology Inc. Dallas, Texas), and for AAC1 the primary antibody was 1:5000 dilution of rabbit IgG anti-AAC1 (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... On the day of the transfection two separate solutions were prepared: Solution A was made by adding 2 μg of plasmid DNA (Santa Cruz Biotechnology ®, sc-405646) into 130 μl of plasmid transfection medium (Santa Cruz Biotechnology ® ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used in this study are as follows: rabbit anti-CTP synthase 1/2 (y88) (1:1000, Santa Cruz Biotechnology, Cat. No. sc-134457) and mouse anti-HTS (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3, VDR, Gfi1) using protein G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA). Eluted conjugates were run on SDS-PAGE and probed with primary antibodies of partner proteins ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated overnight at 4◦C or for 1 h at RT in 2%BSA PBS containing primary antibody mouse anti-lamin B1 (Santa Cruz, sc-365214, 1/500). Cells were washed using PBS and incubated for 1 h at room temperature with secondary antibody in 2% BSA PBS ...
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bioRxiv - Biophysics 2024Quote: ... TDP-43 aggregation levels were detected by GFP fluorescence signal and immunoblotting for GFP using α-GFP (B-2) primary antibody (Santa Cruz Biotechnology, sc-9996) with α-mouse IgG secondary antibody (MBL).
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 or RPE-1 CCNB1AID36 cells were incubated with viral particles in the presence of 8μg/ml polybrene (sc-134220 from Santa Cruz) at 37°C in a 5% CO2 atmosphere for 24hrs ...
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bioRxiv - Neuroscience 2020Quote: Immunocytochemistry was performed using the following primary antibodies: mouse anti-OCT3/4 (2.5 μg/ml, sc-5279, Santa Cruz Biotechnology), mouse anti-TRA-1-60 (1 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with virus-containing supernatants in the presence of 8 μg/mL Polybrene (Santa Cruz Biotech, sc-134220) for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... antibiotic selection after plasmid DNA transfection was performed using 5 μg/ml blasticidin S (3513-03-9, Santa Cruz Biotechnology) or 200 μg/ml of hygromycin B (31282-04-9 ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% Sodium dodecyl sulfate) and analyzed by Western blotting using anti-ISG15 (sc-50366, H150; Santa cruz; 1 μg/mL), anti-Flag (clone M2 Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study are mouse anti-TOM20 directly labeled with AF647 (2.5 μg mL−1, sc-17764-AF647, Santa Cruz) and rabbit anti-PMP70 (1:500 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were infected with lentiviral particles in the presence of 10 µg/ml of polybrene (Cat# sc-134220, Santa Cruz) and selected with puromycin at 3 µg/ml (Cat# 0240.3 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then selected for stable integrants at the FRT locus using 200 μg/ml hygromycin B (Santa Cruz biotechnology) for at least 2 weeks ...
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bioRxiv - Cell Biology 2021Quote: ... HCT116 + CDK1-AF and the corresponding control cells13 were grown in medium with 300 µg/ml G418 (Santa Cruz, USA). All cells were grown in a humidified atmosphere at 37 °C and 5 % CO2.
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bioRxiv - Microbiology 2023Quote: ... A549 or HSAE cells were transduced with the lentiviruses in the presence of 10 µg/mL polybrene (Santa Cruz Biotechnology). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtered viruses were added to culture media of cells with polybrene (Santa Cruz, final concentration of 8 µg/mL). Fresh media were replaced 48 hours after infection ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A-derived 1E7 cells cultured on tissue culture polystyrene were induced with 1 μg/mL doxycycline (204734, Santa Cruz Biotechnology) for 24 hours before being collected with 0.05% trypsin-EDTA.
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies in 2% BSA in PBS overnight at 4°C: mouse anti-PPARγ (Santa Cruz Biotech, sc-7273, 1:1,000), rabbit anti-CEBPα (Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were incubated with primary antibodies in 2% BSA in PBS overnight at 4°C: mouse anti-PPARγ (Santa Cruz Biotech, sc-7273, 1:1,000), rabbit anti-CEBPα (Santa Cruz Biotech ...
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bioRxiv - Immunology 2023Quote: Chromatin from flow-sorted small pre-B cells (2×106) from WT and Brwd1-/- mice were used for each ChIP experiment with anti-BRWD1 (E-15, Santa Cruz Biotechnology, sc-83517, lot J1508), anti-H3K4me1 (07-436 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Biophysics 2019Quote: ... the microspheres were treated at room temperature for 6 hr with 200 μg/mL ICAM1 mAb (G-5, Santa Cruz Biotechnologies). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... All single-cell samples were distributed at 5×105 cells/mL for live staining with mouse anti-human CEA IgG (Santa Cruz) in DPBS with 2% FBS (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... and were first transduced with dCas9 or inducible dCas9 virus medium (thawed from −80 °C) together with 4 μg/mL polybrene (Santa Cruz) to make SNU398-dCas9 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated at 37°C for 30-45 minutes in MEF medium (described in MEF culture section) containing 8µg/ml of Polybrene (Santa Cruz Biotechnology). Ascl1 ...
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bioRxiv - Immunology 2021Quote: ... 1×108 Jurkat T cells were transduced with lentivirus at an MOI of 0.5 with 8 ug/ml polybrene (Santa Cruz Biotechnology) and spinfected at 1000x g for 1.5 hours at 32°C ...
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bioRxiv - Cancer Biology 2020Quote: ... ADA or CD mutants were infected with lentiviral sgRNA targeting PRMT5 in 6 well dishes containing 10 μg/mL polybrene (Santa Cruz). 48 hours after infections ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with pre-determined lentiviral supernatant volume that corresponds to a MOI (multiplicity of infection) of ∼0.3 and 8 μg/ml polybrene (Santa Cruz Biotechnology) and centrifuged at 200 RPM for 45 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... K562 cells were transduced by spinfection at 1,800g for 90 min at 37 °C with 12 μg ml−1 polybrene (Santa Cruz Biotechnology) and the appropriate lentivirus ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were blocked in 10% normal goat serum in PBS for 30 minutes before staining with an anti-AR (N-20) antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816 ...
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bioRxiv - Neuroscience 2020Quote: ... The infected cells were fixed at DIV7 and immunostained with rabbit anti-pan NRXNs (1.6 μg/ml)55 and goat anti-tau (1:1000) (Santa Cruz Biotechnology) antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands probed with diluted primary antibodies (Table S8) were detected using a goat anti-rabbit or mouse IgG-HRP conjugated secondary antibody (200μg/mL; Santa Cruz Biotechnology) and visualized using enhanced chemiluminescence (Amersham Biosciences).