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Citations for Santa Cruz :
9301 - 9350 of 9549 citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Alpha-synuclein was detected using an anti-alpha-synuclein mouse monoclonal antibody (1:500 dilution) (Santa Cruz biotechnology, Santa Cruz, CA, USA). Beta-tubulin was detected using an anti-beta-tubulin mouse monoclonal antibody (E7 ...
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bioRxiv - Genetics 2024Quote: ... Alpha-synuclein was detected using an anti-alpha-synuclein mouse monoclonal antibody (1:500 dilution) (Santa Cruz biotechnology, Santa Cruz, CA, USA). Beta-tubulin was detected using an anti-beta-tubulin mouse monoclonal antibody (E7 ...
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bioRxiv - Developmental Biology 2024Quote: The sources and dilutions of primary antibodies used were as follows: anti-Vasa (goat, 1:100; Santa Cruz Biotechnology, Cat# dc-13); anti-TJ (guinea pig ...
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bioRxiv - Microbiology 2024Quote: ... the formation of a fusion protein with a proper size was validated by Western blotting using anti-myc antibody 9E10 (Santa Cruz Biotechnology), and anti-Xpress tag antibody R91025 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody solution was prepared with 5% (w/v) skimmed milk in TBST with anti-Parp14 mouse monoclonal antibody (1:500, sc377150, Santa Cruz Biotechnology) with enough volume for two membranes (epitope competition vs control) ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were immunoprecipitated using monoclonal mouse anti-WWOX cocktail antibodies53 together with protein A/G plus agarose beads (Santa Cruz, Texas, USA) and incubated overnight at 4°C on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Diluted phage preparations were added and allowed to incubate for 1 hour at RT before captured virions were detected using a 1:5000 dilution of anti-M13-HRP (Santa Cruz Biotechnology) for 1 hour at RT ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were blocked in 5% BSA in PBS for 1 h and incubated overnight at 4℃ with polyclonal goat anti-Cdc25B antibody diluted (1:100, Santa Cruz Biotechnology) and polyclonal rabbit anti-14-3-3ε antibody diluted (1:800 ...
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bioRxiv - Bioengineering 2023Quote: ... the slides were washed 3 times with ice-cold D-PBS to remove unbound antibodies and then incubated with the secondary antibody FITC donkey anti-goat (Santa Cruz Biotechnology) at a 1:200 ratio for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... with the following primary antibodies diluted in blocking solution (3% nonfat milk): goat polyclonal anti-lamin A/C (1:500; sc-6215 Santa Cruz Biotechnology); mouse monoclonal anti-lamin A/C (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... when a western blot was performed on lysate from a house crow brain using an anti-DCX antibody (sc-271390, Santa Cruz Biotechnology). Bands were obtained at similar molecular weights in western blots performed on mouse brain tissue provided by the manufacturer.
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bioRxiv - Physiology 2023Quote: ... The membranes were exposed to primary antibody overnight at 4 C (Phospho eNOS S1179 -BD 612392; Total eNOS/NOS3: BD 610296; anti mouse: Jackson Immuno research 715-035-150; Beta Actin: Santa Cruz sc4778), rinsed and incubated with appropriate horseradish peroxidase-labelled secondary antibody for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cell suspension was then incubated in a positive-selection plate coated with anti-PDGFRα antibodies (catalog no. sc-338; Santa Cruz Biotechnology). The attached cells were collected using trypsin and cultured on poly(L-lysine)-coated coverslips in proliferation medium containing Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Developmental Biology 2023Quote: ... for 1 hour at room temperature and incubated overnight in primary antibody (1:100 anti-Dnmt1 polyclonal, Santa Cruz, 1:1000 H3 polyclonal, Santa Cruz) diluted in blocking buffer at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... For immunohistochemistry and IF staining was performed using DAB or DAPI and the following primary antibodies: anti-ALDH3A1 mouse monoclonal antibody (Santa Cruz Biotechnology), anti-BST2 rabbit polyclonal antibody (Proteintech) ...
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bioRxiv - Cell Biology 2023Quote: ... the aortas were blocked with 5 % BSA for 20 min and stained with anti-Cadherin-5 antibody (Santa Cruz biotechnology, sc-6458) and DAPI ...
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bioRxiv - Genetics 2023Quote: ... QSonica Sonicator Q800R3 was used to shear the chromatin and then equal amounts of DNA were used for immunoprecipitation protocol with anti-Myc (9E10; Santa Cruz biotechnology) with magnetic Protein A beads ...
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bioRxiv - Cell Biology 2023Quote: ... The binding assay was performed in PBST by incubating a constant amount of p53 with an increasing amount of PITPα/β in the presence of 20 μl anti-p53 antibody-conjugated agarose (#sc-126AC, Santa Cruz). After incubating overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween 20) for 1 h at room temperature and stained with mouse monoclonal anti-GFP (1:2000, Santa Cruz #sc-9996) in 3% milk in TBST at 4°C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Alomone Labs) or 5 µM of antibodies for targeting neurons with dopamine receptor 2 (D2R) (anti-D2DR, sc-5303, Santa Cruz Biotechnology) or without cell type specificity (rabbit anti-human IgG ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Selected cryo-sections were then incubated overnight with the following primary antibodies: anti-Shox2 (1:300, Santa Cruz JK-6E, sc-81955), anti-SMA-Cy3 (1:250 ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated with the following primary antibody diluted in 5% milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4°C: anti-RPL22 (1:2000, mouse, Santa Cruz sc-373993), anti-RPS6 (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped with SDS-PAGE sample buffer and analyzed by Western blotting with anti-Gγ2 (7-RE20, Santa Cruz sc-134344) and anti- Gβ1 (Sigma-Aldrich sab2701168).
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bioRxiv - Cell Biology 2024Quote: ... they were blocked with blocking buffer for 2.5 h at RT with continuous shaking and incubated ON with anti-eIF3 (Santa Cruz sc-16377) diluted in blocking buffer at 4°C in a humidified chamber ...
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bioRxiv - Cell Biology 2024Quote: ... the membrane previously incubated with anti-Cyclin B1 antibody was incubated with m-IgGκ BP-HRP secondary antibody (Santa Cruz, sc-516102) for 1 hour at 23°C ...
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bioRxiv - Microbiology 2024Quote: ... Pbs2-6His-myc was immunoprecipitated from 1000 µg of whole extracts following incubation with 40 µl of anti-c-myc agarose (Santa Cruz Biotechnology) for 2 h at 4°C ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... RNA-protein complexes were then immunoprecipitated from the clarified lysate using protein G Dynabeads (Thermo) conjugated to anti-HNRNPC (Santa Cruz sc-32308) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... blocked for 30 min with BSA (1%) in PBS and incubated for 60 min with anti-acetylated-α-tubulin antibody (Santa Cruz Biotechnology) to identify primary cilia.24 Cells were counter-stained with DAPI to locate cell nuclei and mounted with Vectashield mounting medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... by washing 3 times with phosphate-buffer saline (PBS) followed by incubation with either 10 µg of mouse monoclonal anti-Ndc80/Hec1 antibody (Santa Cruz, sc-515550) or 10 µg of mouse IgG (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... the cells were stained according to the same protocol except for the myc staining that was performed using an unconjugated anti-myc tag antibody (Santa Cruz Biotechnology, 9E10) followed by an AMCA anti-mouse antibody (Jackson ImmunoResearch).
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to an Immobilon-P membrane (MilliporeSigma) and probed with either mouse-anti MAL 1:1000 (clone E-1, Santa Cruz Biotechnology, Inc.) or rabbit anti-ICln 1:1000 (PA5-13450 ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Molecular Biology 2021Quote: ... The primary antibodies used for incubation were anti-TGFβ 1/2/3 [H-112] (dilution 1: 80, Santa Cruz Biotechnology, Inc., sc-7892), anti-COX2 antibody [EP1978Y] (dilution 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Drug-resistant colonies were isolated and expression of TIR1-9myc was confirmed by Western blot using anti-Myc antibody (Santa Cruz, sc-40). Clones expressing high level of TIR1-9myc were used for the subsequent experiments.
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bioRxiv - Neuroscience 2019Quote: ... permeabilized in PBS with 10% triton-x for 30 min and incubated for three days at 4 °C with goat anti-DCX antibody (1:250 in 10% triton-x and 3% horse serum (sc-8066; Santa Cruz Biotechnology, USA). Sections were washed and incubated in biotinylated donkey anti-goat secondary antibody for 60 minutes (1:250 ...
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bioRxiv - Genetics 2019Quote: ... Membranes were blocked with 5% milk powder in TBS-T and incubated overnight at 4°C with primary antibodies: anti-GFP mouse antibody (Santa Cruz sc-9996) and anti-PGKD1 mouse antibody (Novex 459250 ...
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bioRxiv - Microbiology 2019Quote: ... Lysate supernatants remaining after the initial PKR immunoprecipitation were then immunoprecipitated again using the same procedure but with 4 µg anti-p53 (DO-1, Santa Cruz sc-126) or 4 µg isotype mouse monoclonal antibodies (ThermoFisher Scientific #02-6200) ...
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bioRxiv - Immunology 2020Quote: ... 1xPBS) and incubated overnight at 4°C using the following dilutions: anti-COPA (1:100, Santa Cruz Biotechnology, clone H-3, sc-398099) and anti-STING (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were probed with anti HA-tag monoclonal antibody (Covance, HA.11, 1:100) while β-Tubulin (Santa Cruz, H-235, 1: 500) was used as a loading control ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were stained overnight at 4°C using unconjugated primary antibodies at 1:100 dilution for endomucin (rat anti-mouse sc-65495, Santa Cruz Biotechnology, Santa Cruz, CA) and at 1:200 dilution for collagen type I (rabbit anti-mouse ...
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bioRxiv - Physiology 2019Quote: ... slides were incubated overnight at 4°C with the following antibodies (diluted in blocking buffer): Anti PECAM-1 (sc-376764, Santa Cruz, 1:50), anti Ki67 (AB9260 ...
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bioRxiv - Cell Biology 2021Quote: The following commercially available antibodies were used at the indicated concentrations for western blot: Anti-ZFP207 (Santa Cruz Biotechnology, sc-271943, 1:500), anti–β-ACTIN (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections were then incubated in a goat polyclonal anti-OXA primary antibody (1:10,000 dilution; SC-8070, Santa Cruz, Dallas, TX, USA) followed by biotinylated horse anti-goat IgG secondary antibody (1:1,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... PDE4 (kindly provided to us by Kenneth Miller, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma), PP1 (sc6104, 1:1000) or an anti-calstabin antibody (Santa Cruz 1: 2,500). To determine channel oxidation ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was washed three times with Tris buffer and incubated for one hour with anti-mouse antibodies conjugated with horseradish peroxidase (Santa Cruz Biotechnology, USA). After washing the membrane ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were reprobed for Gβ by stripping and then incubating overnight with 1:200 diluted mouse monoclonal anti-Gβ antibody (Santa Cruz #sc-166123) followed by a secondary incubation with 1:1000 diluted m-IgGk BP-HRP (Santa Cruz #sc-516102) ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated overnight at 4°C with the primary antibody goat polyclonal anti-GFAP 1:1000 (Santa Cruz, Dallas TX, USA) or rabbit anti-Actin 1:1000 (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Gal-3 and Ki67 expression was detected by IHC using mouse polyclonal anti-Gal-3 (Santa Cruz, CA, USA, SC-14364, 1:500) and rabbit polyclonal anti-Ki67 (abcam ...