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Citations for Santa Cruz :
851 - 900 of 950 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE and immunoblotting was performed as described above with anti-BAP1 antibody (Santa Cruz: sc-28383).
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The following HRP-conjugated secondary antibodies or proteins were used for development of western blots at 1:5000 – 1:10000: mouse IgGκ-binding protein (m-IgGκ BP-HRP) (sc-516102, Santa Cruz); mouse monoclonal anti-rabbit IgG-HRP (sc-2357 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the detection of CDK4 proteins via a quick WB procedure using the sc-601 primary antibody from Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... was from Sigma and the HRP-conjugated anti-mouse IgG-k-chain binding protein (sc-51602) as well as the anti-goat HRP-conjugated secondary antibody (sc-2350) were from Santa Cruz Biotechnology.
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bioRxiv - Plant Biology 2019Quote: ... Expression of bait fusion proteins was confirmed by immunoblotting with anti-c-Myc (clone 9E10, Santa Cruz Biotechnologies, Dallas, TX, USA) and goat anti-mouse IgG-HRP conjugate (Jackson Immuno Research ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] reagent ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were prepared from microdissection of 10 hippocampi of juvenile mice incubated in RIPA lysis buffer (Santa Cruz sc-24948). Active Rac1 Detection was performed using Active Rac1 Detection Kit of Cell Signalling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... for EGFP-tagged Vpu/F1/F2 detection, Nef antibody (EEH1, NIH AIDS Reagent Program) and anti-HA antibody for HA-tagged BST-2 protein (Santa Cruz). Secondary antibodies conjugated to HRP (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... INSR was immunoprecipitated by incubating 500 μg of protein lysate with 2 μg INSR antibodies (Rabbit pAb, Cat. #sc-711, Santa Cruz) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Different primary antibodies were used for the detection of interest proteins: anti-IL-1β rabbit polyclonal (1:1000, H-153, SC-7884, Santa Cruz), anti-caspase-1 (p20 ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently incubated with primary antibodies rabbit anti-Prosurfactant Protein C (pro-SPC) (MilliporeSigma, Burlington, MA, USA, #AB3786, RRID: AB_91588) and mouse anti-Acetylated α Tubulin (ACT) (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... For co-localization studies employing confocal microscopy the following antibody combinations were used: mouse anti-non-structural protein 1 (Santa Cruz)/goat anti-mouse Alexa568 (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1,000, catalog no. sc-9996, Santa Cruz Biotechnology) and anti-RFP antibody (1/2000 ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... anti-S6 ribosomal protein (S6) (mouse monoclonal clone C-8, #sc-74459, RRID: AB_1129205, Santa Cruz Biotechnology; 1:1000 for WB), anti-4E-BP1 (rabbit monoclonal clone 53H11 ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were stripped and re-probed with a polyclonal antibody directed against the immunoprecipitated GRK2 protein (C15, Santa Cruz Biotechnology Inc). Blots were developed using a chemiluminescent method (ECL ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed three-times with TBST (TBS containing 0.1% Tween-20) and incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000; Santa Cruz Biotechnology, USA) for 2 hrs at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The following are the specificities and sources of the commercially available antibodies used in the present study: antimyelin basic protein (catalog #sc-13914, Santa Cruz Biotechnology), anti-NG2 proteoglycan (catalog #AB5320 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein, #516102, Santa Cruz), and the HRP activity was detected by incubation with ECL and exposure to a CCD camera ...
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bioRxiv - Biochemistry 2020Quote: ... Presence of His-tagged protein was detected by chemical luminescence using His-probe (H-3) HRP (sc-8036 HRP, Santa Cruz Biotechnology) monoclonal antibody horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... samples containing same protein amount were subjected to immunoprecipitation with rabbit polyclonal antibody against PDE4B (H-56) (#sc-25812, Santa Cruz Biotechnology) coupled to GammaBind Plus Sepharose beads (# 17-0886-02 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibody membranes were incubated with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:2500) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated in TBST containing 2% (w/v) BSA with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:5000) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 10 μl of a polyclonal ROCK1 antibody (sc-6055, Santa Cruz Biotechnology) coupled to protein G-Sepharose beads (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoprecipitated proteins were separated on SDS-PAGE acrylamide gels and western blots were performed using anti-GFP (Santa Cruz Biotechnology sc-9996), anti-SYT1 [7] ...
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bioRxiv - Plant Biology 2019Quote: ... transferred onto PVDF membrane and proteins were detected using an anti-c-myc monoclonal antibody (sc-40, Santa Cruz Biotech, Texas, USA) and anti-mouse HRP conjugate (W4021 ...
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bioRxiv - Cancer Biology 2021Quote: ... to detect DARPP-32 (Cat no.: sc-135877; Dilution 1:200) and α-Tubulin (Cat no.: sc-5286; Dilution 1:1000) protein were obtained from Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2020Quote: Tissue sections were incubated overnight with polyclonal rabbit antibodies raised against c-fos protein (1:1000; Santa Cruz Biotechnology Inc.; sc-253), GFAP (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Immunology 2023Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg of total protein cell lysate was incubated with 4 µg of rabbit anti-human-ERG Ab (Santa Cruz: #sc-28680) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately 750 µg of solubilized proteins were incubated with 1 µg of anti-C/EBPβ mouse IgG (Santa Cruz Biotech, sc-7962) or normal mouse IgG (Santa Cruz Biotech ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to HRP (1:2000) (sc-516102, Santa Cruz Biotechnology) for 3h at 4°C on a rotary and washed thrice with TBST for 10 min each time ...
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bioRxiv - Microbiology 2024Quote: ... the formation of a fusion protein with a proper size was validated by Western blotting using anti-myc antibody 9E10 (Santa Cruz Biotechnology), and anti-Xpress tag antibody R91025 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Myc- and GFP-tagged proteins were detected by rabbit polyclonal IgG anti-GFP (FL) (1:200; sc-8334 Santa Cruz Biotechnology, Dallas, US) and mouse monoclonal IgG anti-c-Myc (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... A fraction of the mixture of protein-DNA complex was used as “input DNA.” Soluble chromatin was immunoprecipitated with anti-VDRE antibody (D-6, Santa Cruz Biotechnology Inc) and normal mouse immunoglobulin G (IgG ...