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• Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes

  Jiyeon Leem, Jae-Sung Kim, Jeong Su Oh, bioRxiv - Cell Biology 2022

  Quote: ... Control oocytes were injected with normal IgG antibodies (sc-2025; Santa Cruz Biotechnology). Following an additional 4 h of culture after antibody injection ...
• Lomasomes and other fungal plasma membrane macroinvaginations have a tubular and lamellar genesis

  Igor S. Mazheika, Olga V. Kamzolkina, bioRxiv - Cell Biology 2022

  Quote: ... with 25 μM Latrunculin A (LatA; Santa Cruz Biotechnology, USA). The variant without preincubation with mounting with CzM pH 7 has been taken as the basic variant of the sample treatment ...
• P bodies coat germ granules to promote transgenerational gene silencing in C. elegans

  Zhenzhen Du, et al., bioRxiv - Cell Biology 2022

  Quote: ... GFP (1:500, Santa Cruz Biotechnology), mCherry (1:1000 ...
• Parallel Activation of Src and Hif1α Increases Localized Glycolytic ATP Generation for Re-assembly of Endothelial Adherens Junctions

  Li Wang, et al., bioRxiv - Cell Biology 2022

  Quote: ... Src antibody (Santa Cruz Biotechnology, Cat# sc-18), phosphor-Src (Y416 ...
• Parallel Activation of Src and Hif1α Increases Localized Glycolytic ATP Generation for Re-assembly of Endothelial Adherens Junctions

  Li Wang, et al., bioRxiv - Cell Biology 2022

  Quote: ... Myc (Santa Cruz Biotechnology, Cat# sc-40), GFP (Clontech) ...
• Parallel Activation of Src and Hif1α Increases Localized Glycolytic ATP Generation for Re-assembly of Endothelial Adherens Junctions

  Li Wang, et al., bioRxiv - Cell Biology 2022

  Quote: HLMVECs were transfected with siRNAs (si-PFKFB3, si-Hif1α, and nonspecific scramble control siRNA) using siRNA transfection reagent (Santa Cruz Biotech, Cat# sc-29528) according to the manufacturer’s instructions ...
• Parallel Activation of Src and Hif1α Increases Localized Glycolytic ATP Generation for Re-assembly of Endothelial Adherens Junctions

  Li Wang, et al., bioRxiv - Cell Biology 2022

  Quote: ... and polybrene were obtained from Santa Cruz Biotechnology.
• Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor

  Arun Devotta, et al., bioRxiv - Developmental Biology 2022

  Quote: ... and N-terminal (Santa Cruz Biotechnology ...
• Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor

  Arun Devotta, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Each primary antibody was subsequently revealed using donkey anti-goat IgG-HRP (Santa Cruz Biotechnology ...
• Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor

  Arun Devotta, et al., bioRxiv - Developmental Biology 2022

  Quote: ... or anti-Npr3 (Santa Cruz Technology ...
• Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor

  Arun Devotta, et al., bioRxiv - Developmental Biology 2022

  Quote: ... HRP activity was detected with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology, Cat#sc-2048) and imaged on a ChemiDoc MP Biorad gel documentation system (Hercules ...
• Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor

  Arun Devotta, et al., bioRxiv - Developmental Biology 2022

  Quote: ... The blots were then washed and incubated with goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
• The dark kinase STK32A regulates hair cell planar polarity opposite of EMX2 in the developing mouse inner ear

  Shihai Jia, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Goat anti-GPR156 (1:100, TCA, Santa Cruz Biotechnology SC-102572), Rabbit anti-MST4/STK26 (1:500 ...
• The dark kinase STK32A regulates hair cell planar polarity opposite of EMX2 in the developing mouse inner ear

  Shihai Jia, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Goat anti-Oncomodulin/OCN (1:250, PFA, Santa Cruz Biotechnology SC-7466), Rabbit anti-pericentrin/PCNT (1:400 ...
• Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platforms

  Matthew J. Shannon, et al., bioRxiv - Developmental Biology 2022

  Quote: ... mouse monoclonal anti-cytokeratin 7 (clone RCK105; 1:100; Santa Cruz Biotechnology), rabbit monoclonal anti-EGR1 (1:50 ...
• Endothelial TIE1 restricts angiogenic sprouting to coordinate vein assembly in synergy with its homologue TIE2

  Xudong Cao, et al., bioRxiv - Developmental Biology 2022

  Quote: ... including rabbit polyclonal anti-TIE2 (Santa Cruz sc-324), goat polyclonal anti-TIE2 (R&D Systems AF762) ...
• Malt1-dependent cleavage of Tensin-3 controls B-cell adhesion and lymphomagenesis

  Mélanie Juilland, et al., bioRxiv - Immunology 2022

  Quote: ... rabbit anti-Bcl10 (H197, Santa Cruz Biotechnology), anti-Tubulin ...
• Malt1-dependent cleavage of Tensin-3 controls B-cell adhesion and lymphomagenesis

  Mélanie Juilland, et al., bioRxiv - Immunology 2022

  Quote: Antibodies used included polyclonal rabbit anti-human Tensin-3 (C2, Santa Cruz, directed against the C-terminus), affinity purified rabbit anti-human Tensin-3 (generated against a GST-Tensin-3 fusion protein comprising amino acids 418–577 of human Tensin-3) ...
• GBP2 aggregates LPS and activates the caspase-4 inflammasome independent of the bacterial encapsulation factor GBP1

  Mary S. Dickinson, et al., bioRxiv - Immunology 2022

  Quote: ... GBP2 (Santa Cruz sc271568, 1:200), GBP4 (Proteintech 17746-1-AP ...
• Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes

  Mateusz S. Wietecha, et al., bioRxiv - Genomics 2022

  Quote: ... 2) mouse anti-periostin (1:100; Santa Cruz; #sc-398631) and rabbit anti-MFAP4 (1:100 ...
• Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes

  Mateusz S. Wietecha, et al., bioRxiv - Genomics 2022

  Quote: ... anti-lamin A (Santa Cruz, Santa Cruz, CA; #sc-6214), anti-mouse Runx2 (Abcam ...
• Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes

  Mateusz S. Wietecha, et al., bioRxiv - Genomics 2022

  Quote: ... anti-lamin A (Santa Cruz, Santa Cruz, CA; #sc-6214), anti-mouse Runx2 (Abcam ...
• Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes

  Mateusz S. Wietecha, et al., bioRxiv - Genomics 2022

  Quote: ... or 3) goat anti-fibronectin (1:200; Santa Cruz; #sc-6953) and rabbit anti-MFAP4 (1:100 ...
• A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in the human RABEP2 gene modulates ischemic stroke outcomes

  Han Kyu Lee, Do Hoon Kwon, Douglas A. Marchuk, bioRxiv - Genetics 2022

  Quote: ... and mouse anti-GAPDH (sc-32233, Santa Cruz Biotechnology, Inc., TX). HRP-conjugated anti-mouse and anti-rabbit (Santa Cruz Biotechnology ...
• Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe

  Gaspard Kerner, et al., bioRxiv - Genetics 2022

  Quote: ... and GAPDH (#sc-47724, Santa Cruz Biotechnology, 1:5,000). Proteins were detected by chemiluminescence with Clarity Western ECL substrate (#1705061 ...
• A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in the human RABEP2 gene modulates ischemic stroke outcomes

  Han Kyu Lee, Do Hoon Kwon, Douglas A. Marchuk, bioRxiv - Genetics 2022

  Quote: ... HRP-conjugated anti-mouse and anti-rabbit (Santa Cruz Biotechnology, Inc., TX) secondary antibodies were used to detect proteins.
• Fancm regulates meiotic double-strand break repair pathway choice in mammals

  Vanessa Tsui, et al., bioRxiv - Genetics 2022

  Quote: ... Antibodies and dilutions used were: SYCP3 (Santa Cruz, sc-74569 at 1:100); RAD51 (EMD PC130 at 1:250) ...
• Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation

  Jinglong Wang, et al., bioRxiv - Genomics 2022

  Quote: ... Indicated antibodies (anti-Flag, 1:2000, 12793S, Cell Signaling Technology; anti-Rabbit-IgG, 1:2000, G-21234, Thermo Scientific; anti-β-actin, 1:4000, SC-47778, Santa Cruz Biotechnology) and HRP substrate (K-12042-D10 ...
• Chromosome decompaction and cohesin direct Topoisomerase II activity to establish and maintain an unentangled interphase genome

  Erica M. Hildebrand, et al., bioRxiv - Genomics 2022

  Quote: ... Primary antibodies used: mouse anti-Topoisomerase IIα (1:500, Santa Cruz sc-166934), rabbit anti-DNA Topoisomerase IIα and DNA Topoisomerase IIβ (1:10,000 ...
• The genome-wide mutational consequences of DNA hypomethylation

  Nicolle Besselink, et al., bioRxiv - Genomics 2022

  Quote: ... Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz, SC-373750, 1:250) or rabbit polyclonal anti-DNMT1 (Invitrogen ...
• Cold-induced loss of interaction with HSC70 triggers inflammasome activity of familial cold autoinflammatory syndrome-causing mutants of NLRP3

  Akhouri Kishore Raghawan, et al., bioRxiv - Immunology 2022

  Quote: ... IL-1β (SC-7884) and Blotto (sc-2324) were obtained from Santa Cruz Biotechnology ...
• Cold-induced loss of interaction with HSC70 triggers inflammasome activity of familial cold autoinflammatory syndrome-causing mutants of NLRP3

  Akhouri Kishore Raghawan, et al., bioRxiv - Immunology 2022

  Quote: ... HSC70-siRNAs (sc-29349) and control siRNA (sc-37007) were from Santa Cruz Biotechnology ...
• Chromosome level reference genome for European flat oyster (Ostrea edulis L.)

  Manu Kumar Gundappa, et al., bioRxiv - Genomics 2022

  Quote: ... An Omni-C™ library was generated from gill tissue by Dovetail Genomics (Santa Cruz, USA) and sequenced on an Illumina HiSeq X with a paired-end 150 bp configuration.
• Non-neutralizing antibody responses to vesicular stomatitis virus-vectored influenza A virus vaccines correlate with protection

  Kevin Wittwer, et al., bioRxiv - Immunology 2022

  Quote: ... were: bovine α-goat IgG-HRP (1:20,000; Cat#: sc-2384; Santa Cruz Biotechnology), goat α-rabbit IgG Fc conjugated to horseradish peroxidase (HRP ...
• Endosomal GLUT3 is essential for alternative macrophage signaling, polarization, and function

  Dong-Min Yu, et al., bioRxiv - Immunology 2022

  Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...
• BCL6 regulates the endothelial pro-immunogenic phenotype relevant to organ transplant rejection

  Adriana Franco Acevedo, Nicole M Valenzuela, bioRxiv - Immunology 2022

  Quote: ... and mouse anti-STAT1 (Santa Cruz), followed by anti-rabbit-AF647 and anti-mouse-AF488 (Jackson Immuno ...
• Enhancer-promoter contact formation requires RNAPII and antagonizes loop extrusion

  Shu Zhang, et al., bioRxiv - Genomics 2022

  Quote: ... and HSC70 (Santa Cruz, sc-7298; 1:2000), and visualized using the Pierce SuperSignal West Pico ECL kit (Thermo Fisher).
• H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements

  Amanuel Tafessu, et al., bioRxiv - Genomics 2022

  Quote: ... OCT4 (sc-5279, Santa Cruz), NANOG (ab70482 ...
• The genomic basis of evolutionary novelties in a leafhopper

  Zheng Li, et al., bioRxiv - Genomics 2022

  Quote: ... a total of 0.6 g of male and female adult individuals were frozen and shipped to Dovetail Genomics (Santa Cruz, CA, USA). Three male individuals were used for DNA extraction and Pacbio long-read sequencing ...
• E2 ubiquitin conjugase Bendless is essential for PINK1-Park activity to regulate Mitofusin under mitochondrial stress

  Rajit Narayanan Cheramangalam, et al., bioRxiv - Genetics 2022

  Quote: ... Blocking in either 5% Blotto (Santa Cruz sc - 2325) or 5% BSA made in 1X TBS with 0.1% Tween-20 (1X TBSTw20 ...
• Trim28 plays an indispensable role in maintaining functions and transcriptional integrity of hematopoietic stem cells

  Giovannino Silvestri, Chozha Vendan Rathinam, bioRxiv - Immunology 2022

  Quote: ... and 1 mM PMSF (Santa Cruz Biotechnology, Inc.). Cell lysates were boiled with sample buffer (NuPAGE ...
• SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA

  Shovamayee Maharana, et al., bioRxiv - Immunology 2022

  Quote: ... anti-eIF3η (Santa Cruz, sc-137214) and anti-SC35 (Abcam ...
• AGC Kinase Inhibitors Regulate STING Signaling Through SGK-Dependent and SGK-Independent Mechanisms

  Johnny Castillo Cabrera, et al., bioRxiv - Immunology 2022

  Quote: ... 8μg/mL Polybrene (Santa Cruz Biotechnology, sc-134220) and spin infection (1372 RCF for 1hr at RT) ...
• Integrative mapping of the dog epigenome: reference annotation for comparative inter-tissue and cross-species studies

  Keun Hong Son, et al., bioRxiv - Genomics 2022

  Quote: ... and IgG (Santa Cruz Biotechnology, sc-2027) per IP reaction ...
• Cholesterol binds the amphipathic helix of IFITM3 and regulates antiviral activity

  Kazi Rahman, et al., bioRxiv - Immunology 2022

  Quote: ... Membranes were blocked with Odyssey Blocking Buffer (Li-COR) and incubated with the following primary antibodies diluted in Odyssey Antibody Diluent (Li-COR): anti-GST (sc-138; Santa Cruz Biotechnology) and anti-IFITM3 (EPR5242 ...
• Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles

  Hyun Ji Kim, et al., bioRxiv - Genomics 2022

  Quote: ... anti-Apolipoprotein (Santa Cruz Biotechnology, sc-376818) or mouse normal IgG antibodies (Santa Cruz Biotechnology ...
• Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles

  Hyun Ji Kim, et al., bioRxiv - Genomics 2022

  Quote: ... or mouse normal IgG antibodies (Santa Cruz Biotechnology, sc-2025) for 2 h at 4°C ...
• Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure

  Conrad C. Weihl, et al., bioRxiv - Genetics 2022

  Quote: ... anti-rabbit αß-crystallin (Enzo, ADI-SPA-223), anti-rabbit DNAJB4 (proteintech, 13064-1-AP) and anti-human DNAJB4 (Santa Cruz, sc-100711). Rhodamine Phalloidin Reagent (Invitrogen ...
• Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generation

  Michael T Kimble, et al., bioRxiv - Genetics 2022

  Quote: ... The secondary antibody was then added (anti-mouse IgG kappa (Santa Cruz Biotechnology) diluted 1:5000 in blocking solution ...
• Rad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase

  Andrew Ziesel, et al., bioRxiv - Genetics 2022

  Quote: ... Arp7 protein was used a loading control and detected using α-Arp7 primary antibodies (Santa Cruz Biotechnology, #SC8961) with mouse α-goat secondary antibodies (Santa Cruz Biotechnology ...