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• APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus

  Jia Li, Haichao Zhao, Anne McMahon, Shan Yan, bioRxiv - Molecular Biology 2022

  Quote: ... Tubulin (Santa Cruz Biotechnology Cat#sc-8035), YFP (BioVision Cat#3991-100).
• APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus

  Jia Li, Haichao Zhao, Anne McMahon, Shan Yan, bioRxiv - Molecular Biology 2022

  Quote: ... Chk1 (Santa Cruz Biotechnology Cat#sc-8408), Chk1 phosphorylation at Ser345 (Cell Signaling Technology Cat#133D3) ...
• APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus

  Jia Li, Haichao Zhao, Anne McMahon, Shan Yan, bioRxiv - Molecular Biology 2022

  Quote: ... Primary antibodies were purchased from respective vendors: APE1 (Santa Cruz Biotechnology Cat#sc-17774), ATR (Santa Cruz Biotechnology Cat#515173) ...
• The Rab GTPase Ypt1p governs the activation of Unfolded Protein Response (UPR) in Saccharomyces cerevisiae by promoting the preferential nuclear degradation of pre-HAC1 mRNA

  Sunirmal Paira, Anish Chakraborty, Biswadip Das, bioRxiv - Molecular Biology 2022

  Quote: ... Protein APLUS Agarose Beads (Santa Cruz Biotechnology, TX, USA) ∼20 μl bed volume per reaction were equilibrated twice with ten volumes of Buffer A ...
• The Rab GTPase Ypt1p governs the activation of Unfolded Protein Response (UPR) in Saccharomyces cerevisiae by promoting the preferential nuclear degradation of pre-HAC1 mRNA

  Sunirmal Paira, Anish Chakraborty, Biswadip Das, bioRxiv - Molecular Biology 2022

  Quote: ... Protein A sepharose beads (Santa Cruz Biotechnology, TX, USA) were coated for overnight at 4°C using TAP antibody (Cat#CAB1001 ...
• Integrity of membrane fluidity and fatty acid environment are necessary for Ebola virus VP40 assembly and release of viral particles

  Souad Amiar, et al., bioRxiv - Microbiology 2022

  Quote: ... HRP-conjugated mouse anti-β-actin antibody (Santa Cruz Biotechnologies, sc-47778), mouse anti-Caveolin 1 antibody (Cell Signaling Technology ...
• Attenuation hotspots in neurotropic human astroviruses

  Hashim Ali, et al., bioRxiv - Microbiology 2022

  Quote: ... The capsid protein of HAstV4 was detected using astrovirus 8E7 antibody (Santa Cruz Biotechnology, sc-53559, 1:750).
• Mucosal and systemic neutralizing antibodies to norovirus and rotavirus by oral immunization with recombinant rotavirus in infant mice

  Takahiro Kawagishi, et al., bioRxiv - Microbiology 2022

  Quote: ... plus 50 µM ceramide (C2; Santa Cruz Biotechnology, cat# sc-201375A) and mixed with 100 TCID50/100 μl of GII.4 Sydney [P16] (GenBank # OL898515 ...
• Mucosal and systemic neutralizing antibodies to norovirus and rotavirus by oral immunization with recombinant rotavirus in infant mice

  Takahiro Kawagishi, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-RV capsid protein mouse monoclonal antibody (Santa Cruz Biotechnology ...
• Coordinated and distinct LD-transpeptidase-independent roles of peptidoglycan carboxypeptidases DacC and DacA in stress adaptation and cell shape maintenance

  Umji Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... and DacA(ΔC) were determined using a monoclonal antibody against Flag-tag (Santa Cruz Biotechnology), according to the standard procedure.
• Coordinated and distinct LD-transpeptidase-independent roles of peptidoglycan carboxypeptidases DacC and DacA in stress adaptation and cell shape maintenance

  Umji Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... The protein levels of PG carboxypeptidases and DnaK were determined using monoclonal antibodies against Flag-tag (Santa Cruz Biotechnology, USA) and anti-DnaK (Abcam ...
• Real-time visualization of phagosomal pH manipulation by Cryptococcus neoformans in an immune signal-dependent way

  Emmanuel J. Santiago-Burgos, et al., bioRxiv - Microbiology 2022

  Quote: ... Cells were immunolabeled in 1% goat serum at 4 °C overnight with an antibody against Gal3 (rat; M3/38: sc23938, Santa Cruz Biotechnology), or against CD86 (PA5-88284 ...
• Coordinated and distinct LD-transpeptidase-independent roles of peptidoglycan carboxypeptidases DacC and DacA in stress adaptation and cell shape maintenance

  Umji Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... The protein level of DacC was detected using a monoclonal antibody against the Flag-tag (Santa Cruz Biotechnology), according to the western blot protocol described above.
• Omicron-induced interferon signalling prevents influenza A virus infection

  Denisa Bojkova, et al., bioRxiv - Microbiology 2022

  Quote: ... ISG15 (Santa Cruz Biotechnology, #sc-166755, 1:200), MDA5 (Cell Signaling ...
• Longitudinal characterization of circulating extracellular vesicles and small RNA during simian immunodeficiency virus infection and antiretroviral therapy

  Yiyao Huang, et al., bioRxiv - Microbiology 2022

  Quote: ... CD81 (1:500, Santa Cruz Biotechnology sc23962), calnexin (1:2000 ...
• Cell Senescence-Independent Ageing of Human Skin

  J. Wordsworth, et al., bioRxiv - Molecular Biology 2022

  Quote: ... LTBP2 (1:250 Santa Cruz, 166199), TAGLN (1:2000 ...
• A living conductive marine biofilm engineered to sense and respond to small molecules

  Lina J. Bird, et al., bioRxiv - Microbiology 2022

  Quote: ... DAPG (Santa Cruz Biotechnology, Dallas, TX) was added from stock solutions prepared in dimethyl formamide ...
• BNIP3 regulates Chikungunya virus infection independently of autophagy and cell death

  Liliana Echavarria-Consuegra, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-Tom20 (rabbit polyclonal, FL-145, Santa Cruz), anti-GAPDH (mouse monoclonal ...
• BNIP3 regulates Chikungunya virus infection independently of autophagy and cell death

  Liliana Echavarria-Consuegra, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-BNIP3 (mouse monoclonal, ANa40, Santa Cruz), anti-BNIP3 (rabbit ...
• Oligodendrocyte precursor cells stop sensory axons regenerating into the spinal cord

  Hyukmin Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... Secondary antibodies for Western blots were HRP-conjugated goat anti-rabbit antibody (Santa Cruz, SC2030; 1:10,000), HRP-conjugated goat anti-mouse antibody (Jackson ImmunoResearch Laboratories ...
• G protein-biased LPAR1 agonism promotes prototypic antidepressant effects

  Naoto Kajitani, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-β-arrestin1/2 antibody (sc-74591, Santa Cruz, CA, USA), and anti-Na+/K+-ATPase antibody (#3010 ...
• Rescue of neuropsychiatric phenotypes in a mouse model of 16p11.2 duplication syndrome by genetic correction of an epilepsy network hub

  Marc P. Forrest, et al., bioRxiv - Neuroscience 2022

  Quote: ... and non-specific rabbit IgG antibody (Santa Cruz, sc-2027) was crosslinked to 30 μl of packed Protein A/G beads (Pierce # 20421) ...
• Rescue of neuropsychiatric phenotypes in a mouse model of 16p11.2 duplication syndrome by genetic correction of an epilepsy network hub

  Marc P. Forrest, et al., bioRxiv - Neuroscience 2022

  Quote: ... ERK1 (Santa Cruz, sc94, 1:5000), and TAOK1 (Santa Cruz ...
• Rescue of neuropsychiatric phenotypes in a mouse model of 16p11.2 duplication syndrome by genetic correction of an epilepsy network hub

  Marc P. Forrest, et al., bioRxiv - Neuroscience 2022

  Quote: ... or 1 μg of mouse IgG (sc-2025, Santa Cruz) overnight at 4_°C ...
• Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneurons

  Zouzana Kounoupa, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse monoclonal anti-Lamp-1 (1:50; Santa Cruz Biotechnology, California, USA), rabbit polyclonal anti-Pericentrin (1:500 ...
• Growth hormone receptor (GHR) in AgRP neurons regulates thermogenesis in aged mice in a sex-specific manner

  Lukas Stilgenbauer, et al., bioRxiv - Neuroscience 2022

  Quote: ... then the staining was carried out with the mouse anti-cFos (anti-rabbit, 1:500, cat. number sc-52, Santa Cruz). Immunostaining brain sections were pretreated with 0.5% NaOH and 0.5% H2O2 in PBS for 20 min ...
• Transcriptional control of motor pool formation and motor circuit connectivity by the LIM-HD protein Isl2

  Yunjeong Lee, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-Isl2 (Santa Cruz), guinea pig anti-Lhx3 (Thaler et al. ...
• A primary sensory cortical interareal feedforward inhibitory circuit for tacto-visual integration

  Simon Weiler, et al., bioRxiv - Neuroscience 2022

  Quote: ... and goat-anti-SOM (1:100, Santa cruz). Following secondary antibodies were used ...
• A primary sensory cortical interareal feedforward inhibitory circuit for tacto-visual integration

  Simon Weiler, et al., bioRxiv - Neuroscience 2022

  Quote: ... sections were incubated free floating with a primary antibody against c-fos (rabbit-anti-c-fos, 1:250, Santa Cruz) over night and at room temperature ...
• A primary sensory cortical interareal feedforward inhibitory circuit for tacto-visual integration

  Simon Weiler, et al., bioRxiv - Neuroscience 2022

  Quote: ... Following primary antibodies were used: rabbit-anti-c-fos (1:200, Santa Cruz), rabbit-anti-PV (1:1000 ...
• Twist1 and balanced retinoic acid signaling act to suppress cortical folding in mice

  Khue-Tu Ho-Nguyen, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-Twist1 (1:50, Santa Cruz), mouse antiCrapb2 (1:200 ...
• Twist1 and balanced retinoic acid signaling act to suppress cortical folding in mice

  Khue-Tu Ho-Nguyen, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-connexin 43 (1:200, Santa Cruz), rabbit anti-connexin 43 (1:1000 ...
• Rescue of neuropsychiatric phenotypes in a mouse model of 16p11.2 duplication syndrome by genetic correction of an epilepsy network hub

  Marc P. Forrest, et al., bioRxiv - Neuroscience 2022

  Quote: ... and TAOK1 (Santa Cruz, sc-47447, 1:500) antibodies
• Retinitis pigmentosa associated mutations in mouse Prpf8 cause misexpression of circRNAs and degeneration of cerebellar granule neurons

  Michaela Krausová, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-IP3R-I/II/III (sc-377518, Santa Cruz), anti-NeuN (#24307 ...
• Large scale interrogation of retinal cell functions by 1-photon light-sheet microscopy

  Suva Roy, et al., bioRxiv - Neuroscience 2022

  Quote: ... The retinas were fixed in 4% PFA for 45 minutes at room temperature and then incubated in 5% normal donkey serum (Jackson Immuno, C840D36) in 1X phosphate buffer saline (PBS) with azide (Santa Cruz Biotechnology, SC-296028) containing 0.5% Triton X-100 (Sigma Aldrich ...
• Machine learning approaches based on fibroblast morphometry confidently identify stress but have limited ability to predict ALS

  Csaba Konrad, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti G3BP1 mouse (Santa cruz, cat sc-81940, 1:200) and anti HSP60 (Enzo ...
• Signal Peptide Peptidase-Like 2b affects APP cleavage and exhibits a biphasic Aβ-mediated expression in Alzheimer’s disease

  Riccardo Maccioni, et al., bioRxiv - Neuroscience 2022

  Quote: The SPPL2b gene was knocked out in HEK293 WT cells by using the SPPL2b CRISPR/Cas9 Knockout Plasmid kit from Santa Cruz Biotechnology® (sc-405646) ...
• Signal Peptide Peptidase-Like 2b affects APP cleavage and exhibits a biphasic Aβ-mediated expression in Alzheimer’s disease

  Riccardo Maccioni, et al., bioRxiv - Neuroscience 2022

  Quote: ... Solution B was made by adding 10 μl of Ultracruz® transfection reagent (Santa Cruz Biotechnology ®, sc-395739) into 140 μl of plasmid transfection medium (Santa Cruz Biotechnology ® ...
• Signal Peptide Peptidase-Like 2b affects APP cleavage and exhibits a biphasic Aβ-mediated expression in Alzheimer’s disease

  Riccardo Maccioni, et al., bioRxiv - Neuroscience 2022

  Quote: ... On the day of the transfection two separate solutions were prepared: Solution A was made by adding 2 μg of plasmid DNA (Santa Cruz Biotechnology ®, sc-405646) into 130 μl of plasmid transfection medium (Santa Cruz Biotechnology ® ...
• Signal Peptide Peptidase-Like 2b affects APP cleavage and exhibits a biphasic Aβ-mediated expression in Alzheimer’s disease

  Riccardo Maccioni, et al., bioRxiv - Neuroscience 2022

  Quote: ... into 130 μl of plasmid transfection medium (Santa Cruz Biotechnology ®, sc-108062); Solution B was made by adding 10 μl of Ultracruz® transfection reagent (Santa Cruz Biotechnology ® ...
• Signal Peptide Peptidase-Like 2b affects APP cleavage and exhibits a biphasic Aβ-mediated expression in Alzheimer’s disease

  Riccardo Maccioni, et al., bioRxiv - Neuroscience 2022

  Quote: ... into 140 μl of plasmid transfection medium (Santa Cruz Biotechnology ®, sc-108062). Both solutions were left at RT for at least 5 minutes and were subsequently combined ...
• Important anatomical, age-related, and species considerations regarding ocular fibulin-3 (EFEMP1) analysis

  Steffi Daniel, John D. Hulleman, bioRxiv - Neuroscience 2022

  Quote: ... The antibodies used were as follows: anti-fibulin-3 antibody (1:200, Santa Cruz, #sc-365224, Dallas, TX), anti-fibulin-3 antibody (1:100 ...
• Large scale interrogation of retinal cell functions by 1-photon light-sheet microscopy

  Suva Roy, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-PCP2 (1:500, Santa Cruz Biotechnology, Inc. sc-137064). Secondary antibodies conjugated to Alexa 488 (1:500 ...
• Loss of function in the autism and schizophrenia-associated gene CYFIP1 in human microglia supports a role in synaptic pruning

  Steven D. Sheridan, et al., bioRxiv - Neuroscience 2022

  Quote: ... iPSCs were passaged as needed in the presence of 2 μM Rho-associated kinase (ROCK) inhibitor (RI) thiazovivin (Santa Cruz Biotechnology, #SC-361380). Prior to transfection ...
• Effective NGN2-based neuronal programming of hiPSCs in an automated microfluidic platform

  S Angiolillo, S Micheli, O Gagliano, bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-OCT4-3/4 (C-10) (1:200, sc-5279 Santa Cruz Biotechnology) and mouse anti-TUBULIN β 3 (βIIITUBB ...
• Retinitis pigmentosa associated mutations in mouse Prpf8 cause misexpression of circRNAs and degeneration of cerebellar granule neurons

  Michaela Krausová, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Prpf6 (#sc-166889, Santa Cruz Biotechnology), anti-Prpf8 (#79237 ...
• Retinitis pigmentosa associated mutations in mouse Prpf8 cause misexpression of circRNAs and degeneration of cerebellar granule neurons

  Michaela Krausová, et al., bioRxiv - Neuroscience 2022

  Quote: ... 30 μl of Protein G agarose beads (#sc-2002, Santa Cruz Biotechnology) were then added to the mixture ...
• Optogenetic manipulation of second messengers in neurons and cardiomyocytes with microbial rhodopsins and adenylyl cyclase

  Hanako Hagio, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Myc Tag (MT) antibody (1:500, mouse, Santa Cruz Biotechnology ...
• Huntingtin expression influences spontaneous seizure disorder susceptibility in FVN/B mice

  Jeremy M. Van Raamsdonk, et al., bioRxiv - Neuroscience 2022

  Quote: ... CA) or rabbit polyclonal anti-BDNF antibody (1:1000, overnight, 4 °C; Santa Cruz, Santa Cruz, CA).
• Huntingtin expression influences spontaneous seizure disorder susceptibility in FVN/B mice

  Jeremy M. Van Raamsdonk, et al., bioRxiv - Neuroscience 2022

  Quote: ... or acH4 which recognizes acetylated Lys5, Lys8, Lys12 and Lys16 (Upstate Biotechnology, cat. #06-866) or an equivalent amount of control IgG (anti-rabbit, Santa Cruz, CA) at 4°C overnight ...