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Citations for Santa Cruz :
701 - 750 of 8085 citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... ChIP reactions were performed by incubating lysates overnight at 4°C with 1ng of Goat anti-Su(H) (Santa Cruz Biotechnology, sc15813), and immunocomplexes were then isolated with Protein G Agarose for 2h ...
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bioRxiv - Physiology 2021Quote: ... adenovirus was added to the medium for 3 hours on day 4 of differentiation at 250 MOI in the presence of 5 μg/ml polybrene (Santa Cruz Biotechnology). Experiments were performed 3 days later ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 500 µL of cold PBS 1X and fixed by adding 4.5 mL of 4 % paraformaldehyde in saline (sc-281692, Santa Cruz Biotechnology, France) and incubating at room temperature for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... Bacterial infection foci were allowed to develop for 24 h prior to fixation with 4% paraformaldehyde (PFA) solution (Santa Cruz Biotechnology, Inc) for 20 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were then incubated with primary antibody in a 1:1000 dilution with 5% BSA in TBST overnight at 4°C: tyrosine phosphorylation using phosphotyrosine mouse monoclonal Ig pY99 (Santa Cruz Biotechnologies); mCherry expression using anti-mCherry rabbit polyclonal Ig (Rockland Inc.) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred onto a PVDF membrane and probed with one of the following antibodies (4 °C, overnight): Anti-His (#SC-8036; Santa Cruz biotechnology) at 1:700 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... incubated 1h at 25°C in 1% BSA-0.3% Triton X-100-TBS solution and then kept at 4°C in rabbit anti-c-fos antibody (sc-52 LotG1108, Santa Cruz Biotechnology) solution (1:200 in 1% BSA-TBS ...
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bioRxiv - Genomics 2024Quote: ... lentiviral transduction was performed by mixing cells in media supplemented with 8 µg/mL of polybrene (Santa Cruz Biotechnology 28728-55-4) with lentivirus and centrifuging in cell culture plates at 1000 xg and 33 °C for 2 hours ...
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bioRxiv - Bioengineering 2024Quote: ... The corneal and skin samples collected were further fixed using 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS; Santa Cruz, sc-281692) at 4 °C for 24 hours ...
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bioRxiv - Biochemistry 2023Quote: ... 80% confluent RHMVECs in 6-well plates were transduced with human T111I EL (pKS18) lentiviruses with 4 μg/ml Polybrene (#134220; Santa Cruz Biotechnology) in a total volume of 1 ml DMEM ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... ONCs were washed and fixed in 4% paraformaldehyde and incubated with a rabbit polyclonal anti-myc antibody (Santa Cruz Biotech, sc-789) in permeabilizing/blocking solution (0.5% Triton X ...
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bioRxiv - Plant Biology 2024Quote: ... This diluted chromatin was then incubated overnight at 4°C with Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology; sc-2003) and antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Microbiology 2024Quote: ... larvae were treated with 10 µL of different drug combinations including FLC (4 mg/kg) + antagonizing compound: carvedilol (sc-200157, Santa Cruz Biotechnology), desogestrel (Y0000509 ...
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bioRxiv - Biochemistry 2024Quote: ... The mixture was rocked for an hour at 4°C and then purified in a CrystalCruz chromatography column (Santa Cruz, sc-205554) at 4°C ...
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bioRxiv - Genomics 2020Quote: Sonicated chromatin was pre-cleared overnight at 4°C with 8 μg of normal goat IgG (Santa Cruz Biotechnology, Santa Cruz, CA; sc2028) for TAL1 or 8 μg of normal rabbit IgG (sc2027 ...
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bioRxiv - Genomics 2020Quote: Sonicated chromatin was pre-cleared overnight at 4°C with 8 μg of normal goat IgG (Santa Cruz Biotechnology, Santa Cruz, CA; sc2028) for TAL1 or 8 μg of normal rabbit IgG (sc2027 ...
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bioRxiv - Molecular Biology 2021Quote: ... The culture media was filtered through a 0.45-μm filter and added to A549 cells along with Polybrene (4 μg/ml; Santa Cruz Biotechnology, Dallas, TX). At 72 h after transduction ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×105 cells were infected in the presence of 1.5 ml of viral supernatant and 4 μg/ml polybrene (Santa Cruz Biotechnology, SC-134220).
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 20 h at 4 °C in the blocking buffer with goat IgG antibodies specific for the human AIF protein (Santa Cruz Biotechnology, USA) diluted 1:200 ...
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bioRxiv - Neuroscience 2022Quote: The rats were euthanized with an overdose of carbon dioxide and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (Santa Cruz Biotechnology Inc.). Fixed brains were cut in 20-μm sections ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653) in the dark at room temperature for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Growth media was removed, and monolayers were washed with PBS (AmericanBio, Inc) prior to fixation with 4% PFA solution (Santa Cruz Biotechnology, Inc) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Neuroscience 2024Quote: ... Then 10% of the lysate was set aside for use as an input control and the rest was tumbled overnight at 4°C with 7 µl of ERα antibody or 1.4 µg of Homer antibody (Santa Cruz Biotechnology, D-3). The same isotype IgG was used as control for the specificity of the ERα (Abcam #ab172730 ...
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bioRxiv - Biochemistry 2023Quote: ... incubated the supernatant with corresponding antibody for 12h at 4°C and then incubated with A/G plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed the next day with 4% paraformaldehyde, permeabilized with 0.25% triton-x and stained with p-Paxillin (CST, 69363) and YAP (Santa Cruz Biotechnology, sc-101199). Images were captured on Zeiss LSM 800 Axio Inverted confocal microscope (Plan-Apochromat 63x/1.40 oil).
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bioRxiv - Genetics 2024Quote: ... The membrane was briefly washed with 1X TBST and incubated overnight with the primary antibody dissolved in 1X TBST at 4°C on slow shaking (ADAM3 from Santa Cruz Biotechnology (sc365288) and COXIV from Cell Signaling Technology (4844s)) ...
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bioRxiv - Cancer Biology 2024Quote: ... The culture media was filtered through a 0.45-μm filter and added to the cells with polybrene (4 µg/ml; Santa Cruz Biotechnology, Dallas, TX). At 72 hours after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 12,000 × g for 5 minutes and then incubated with anti-myosin 5A (G-4) antibody (sc-365986, 0.75 μg/ml, mouse monoclonal; Santa Cruz Biotechnology, Dallas, TX, USA) conjugated to PE-Cy7 (ab102903 ...
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bioRxiv - Immunology 2022Quote: ... Next the membranes were washed for 5 min X 4 times with PBST and incubated with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, SantaCruz, CA) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with the antibodies for 1h at 4°C on a roller before 80µl Protein G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA) were added and samples were incubated overnight at 4°C on a roller ...
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bioRxiv - Developmental Biology 2021Quote: ... and 30 μg/lane of sample were separated by 4–15% gradient Trisacetate SDS-PAGE and electro transferred to PVDF membrane (Santa Cruz Biotechnology, sc-3723). The blots were probed with individual primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:1000 in 5% non-fat dry milk in PBST1X and incubated o/n at 4°C and then with Goat anti-rabbit (cat. SC-2004 – Santa Cruz, Dallas, TX, USA) diluted 1:5000 in 2% non-fat dry milk in PBST1X 1h RT ...
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bioRxiv - Microbiology 2021Quote: ... solution overnight at 4°C followed by a horseradish peroxidase-conjugated mouse anti-rabbit IgG secondary antibody (Cat# sc-235, Santa Cruz, Dallas, TX, USA) diluted 1:3000 in TBST for 1 hour at room temperature with agitation ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 16 hours at 37°C in 5% CO2 then fixed in 4% paraformaldehyde in PBS pH 7.4 (Santa Cruz, Cat. No. sc-281692) with 10 μg/ml of Hoechst (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Blots were then washed three times for 15 minutes at 4 °C with 0.05% TBS-Tween and stained for 4 hours with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) (Santa Cruz Biotech, sc-516102) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: Mice were first anesthetized using isoflurane followed by perfusion of the brain with ice cold PBS followed by 4% Paraformaldehyde (PFA: sc-281692, Santa Cruz Biotechnology, Inc., CA) in PBS ...
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bioRxiv - Neuroscience 2023Quote: All rats were perfused at the end of the experiment with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Santa Cruz Biotechnology Inc., CA). Brains were cut in 40 μm sections and stained with thionin.
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bioRxiv - Neuroscience 2023Quote: iPSC clones were extensively characterised.27 Putative iPSC were analyzed for the expression of the pluripotency-associated markers octamer-binding transcription factor 4 (OCT4, Santa Cruz Biotechnology, Dallas, USA), TRA-1-60 (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then centrifuged for 5 min at 340 x g and resuspended with 200μL of 4% PFA in PBS (Santa Cruz Biotech, cat# sc-281692). All samples were acquired with a MACSQuant Analyzer 10 flow cytometer and analyzed with FloJo software version 10.4.0 (Tree Star) ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed twice with DPBS to wash out the non-membrane bound GFP-LC3B out of the permeabilized cells and fixated with 4% paraformaldehyde (PFA) (Santa Cruz Biotechnology, #sc-281692). The mean fluorescence intensity (MFI ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated with primary antibody or rabbit IgG (control) for 3hr at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 35min with head-to-tail rotation either in the absence or presence of 100 µM CCT blocking peptide SAGRRFIVSPVPE (United Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... were seeded at 70–80 % confluence in a 6-well plate and transiently transfected 4-6 h later with the TST shRNA plasmid (sc-36418-SH : Santa Cruz Biotechnology Inc., Santa Cruz, CA 95060), a packaging plasmid pLP1 ...
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bioRxiv - Cell Biology 2020Quote: ... Membrane-immobilized proteins were probed with mouse anti-linker histone H1 monoclonal antibody clone AE-4 (Upstate Biotechnology, Lake Placid, NY, USA) or goat anti-actin antibody clone I-19 (Santa Cruz Biotechnology, Dallas, TX, USA) and visualized with horseradish peroxidase-conjugated rabbit anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with primary antibody solutions overnight at 4 °C (p21, mouse, cat#556430 from BD; p53, mouse, cat#SC-126 from Santa Cruz Biotechnology; Phospho-p53 (Ser15), rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 0.5-1x105 cells were seeded on coated cover glasses for 30 minutes and then fixed with chilled 4% paraformaldehyde (Santa Cruz Biotechnology, Dallas, TX, USA) for 10 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... The oocytes were incubated overnight in 4 °C in a drop of primary antibodies rat monoclonal anti-CD9 (KMC8.8) (sc18869, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) diluted 1:50 in 1% BSA in PBS and rabbit polyclonal anti-Folate receptor 4 (Juno ...