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• Substrate trapping approach identifies TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes

  Emily Yang, et al., bioRxiv - Cell Biology 2022

  Quote: ... 1:500 anti-G3BP1 (Santa Cruz, Dallas, TX), 1:1000 anti-G3BP2 (Assay Biotech ...
• Cellular redox metabolism is modulated by the distinct localization of cyclic nucleotide phosphodiesterase 5A isoforms

  Silvia Cardarelli, et al., bioRxiv - Cell Biology 2022

  Quote: ... Blots were incubated overnight at 4 °C with rabbit polyclonal anti-PDE5 antibody (1:1000 v/v; Santa Cruz Biotechnology, # sc-32884). Horseradish peroxidase conjugated anti-rabbit IgG (1:10000 v/v ...
• A partnership of the lipid scramblase XK and of the lipid transfer protein VPS13A at the plasma membrane

  Andrés Guillén Samander, et al., bioRxiv - Cell Biology 2022

  Quote: ... Primary (anti-c-Myc, 9E10; Santa Cruz) and secondary (Goat anti-Mouse IgG ...
• In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair

  Sankalp Shukla, et al., bioRxiv - Cell Biology 2022

  Quote: ... using the primary-conjugated Mouse Monoclonal Antibody (no. sc-7964 AF647, Santa Cruz Biotechnology), and Gal3 ...
• In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair

  Sankalp Shukla, et al., bioRxiv - Cell Biology 2022

  Quote: ... using the primary-conjugated Mouse Monoclonal Antibody (no. sc-32790 AF594, Santa Cruz Biotechnology). ALG-2 and ALIX antibodies were diluted 1:200 with 2% BSA in PBS and incubated with cells for 1 hour at room temperature.
• The role of Cycloastragenol at the intersection of Nrf-2/ARE, telomerase, and proteasome activity

  Sinem Yılmaz, et al., bioRxiv - Cell Biology 2022

  Quote: ... FKBP52 (Santa Cruz, sc-100758) and p16 (CST ...
• The role of Cycloastragenol at the intersection of Nrf-2/ARE, telomerase, and proteasome activity

  Sinem Yılmaz, et al., bioRxiv - Cell Biology 2022

  Quote: ... Hsp90 (Santa Cruz, sc-69703), p23 (Santa Cruz ...
• The role of Cycloastragenol at the intersection of Nrf-2/ARE, telomerase, and proteasome activity

  Sinem Yılmaz, et al., bioRxiv - Cell Biology 2022

  Quote: ... p23 (Santa Cruz, sc-101496), CHIP (Santa Cruz ...
• The role of Cycloastragenol at the intersection of Nrf-2/ARE, telomerase, and proteasome activity

  Sinem Yılmaz, et al., bioRxiv - Cell Biology 2022

  Quote: ... CHIP (Santa Cruz, sc-133066), FKBP52 (Santa Cruz ...
• Aging-related iron deposit prevents the benefits of HRT from late postmenopausal atherosclerosis

  Tianze Xu, et al., bioRxiv - Cell Biology 2022

  Quote: ... The ERα antibody was the same as that used for Western blotting and was purchased from Santa Cruz (1:200 dilution). Western blotting was used to detect the efficiency of IP and the level of ubiquitin.
• Wolbachia action in the sperm produces developmentally deferred chromosome segregation defects during the Drosophila mid-blastula transition

  Brandt Warecki, et al., bioRxiv - Cell Biology 2022

  Quote: ... Read alignment and processing was performed using the Hummingbird Computational Cluster (UC Santa Cruz, Santa Cruz, CA, USA). Gene coordinate positions were determined in the UCSC Genome Browser.
• Wolbachia action in the sperm produces developmentally deferred chromosome segregation defects during the Drosophila mid-blastula transition

  Brandt Warecki, et al., bioRxiv - Cell Biology 2022

  Quote: ... Read alignment and processing was performed using the Hummingbird Computational Cluster (UC Santa Cruz, Santa Cruz, CA, USA). Gene coordinate positions were determined in the UCSC Genome Browser.
• Damaged mitochondria recruit the effector NEMO to activate NF-κB signaling

  Olivia Harding, Erika L.F. Holzbaur, bioRxiv - Cell Biology 2022

  Quote: ... anti-TOM20 (Santa Cruz, sc-17764, 1:50), anti-p62 (abcam ...
• Polyalanine disease mutations impair UBA6-dependent ubiquitination

  Fatima Amer-Sarsour, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-TH (Santa Cruz 25269); mouse anti-SOX10 (Abcam 155279) ...
• Polyalanine disease mutations impair UBA6-dependent ubiquitination

  Fatima Amer-Sarsour, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-PRPH (Santa Cruz 377093); mouse anti-TH (Santa Cruz 25269) ...
• Polyalanine disease mutations impair UBA6-dependent ubiquitination

  Fatima Amer-Sarsour, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-MATH-1 (Santa Cruz 136173), mouse anti-PRPH (Santa Cruz 377093) ...
• Polyalanine disease mutations impair UBA6-dependent ubiquitination

  Fatima Amer-Sarsour, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-E6-AP (Santa Cruz 166689); mouse anti-FLAG (SIGMA F1804) ...
• Polyalanine disease mutations impair UBA6-dependent ubiquitination

  Fatima Amer-Sarsour, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-PHOX2B (Santa Cruz 376997); mouse anti-mCherry (Abcam 125096) ...
• Polyalanine disease mutations impair UBA6-dependent ubiquitination

  Fatima Amer-Sarsour, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-Tau (Santa Cruz 58860); rabbit anti-MAP2a (synaptic systems 188003) ...
• Endogenous Rab38 regulates LRRK2’s membrane recruitment and substrate Rab phosphorylation in melanocytes

  Alexandra Unapanta, et al., bioRxiv - Cell Biology 2022

  Quote: ... Coverslips were stained with the following primary antibodies: mouse-anti Rab32 (Santa Cruz, 390178), rabbit anti-Rab38 (gift from Santiago Di Pietro),46 mouse anti-Rab10 (Sigma ...
• Endogenous Rab38 regulates LRRK2’s membrane recruitment and substrate Rab phosphorylation in melanocytes

  Alexandra Unapanta, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-Rab8A (Santa Cruz, 81909), rabbit anti-Rab8A phospho T72 utilized as a pan-phospho-Rab antibody (Abcam ...
• Endogenous Rab38 regulates LRRK2’s membrane recruitment and substrate Rab phosphorylation in melanocytes

  Alexandra Unapanta, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-Rab32 (Santa Cruz, 390178), rabbit anti-Rab29 (Abcam ...
• MLL family members regulate H3K4 methylation to ensure CENP-A assembly at human centromeres

  Kausika Kumar Malik, et al., bioRxiv - Cell Biology 2022

  Quote: ... total RNA Polymerase II (sc-9001, Santa Cruz Technology), and RNA polymerase IIS2P (ab24758 ...
• Diet suppresses tumour initiation by maintaining quiescence of mutation-bearing neural stem cells

  Valeria Amodeo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... EdU (Santa Cruz, sc-284628) was administered in the drinking water (0.2 mg/ml ...
• The chromatin associated Sin3B is a critical regulator of DNA damage repair through the engagement of the Non-Homologous End Joining

  Jorge Morales-Valencia, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse anti-Lamin A/C (Santa Cruz sc-376248).
• The chromatin associated Sin3B is a critical regulator of DNA damage repair through the engagement of the Non-Homologous End Joining

  Jorge Morales-Valencia, et al., bioRxiv - Cancer Biology 2022

  Quote: ... rabbit anti-Sin3B (Santa Cruz AK-12), rabbit anti-γ-H2AX (Cell Signaling 9718) ...
• The chromatin associated Sin3B is a critical regulator of DNA damage repair through the engagement of the Non-Homologous End Joining

  Jorge Morales-Valencia, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 9610-5-D)/protein-A agarose (Santa Cruz sc-2003) and incubated with H4K16Ac antibody (Millipore 07-329)-protein A agarose overnight at 4°C ...
• Hepatocytes deficient in nuclear envelope protein lamina-associated polypeptide 1 are an ideal mammalian system to study intranuclear lipid droplets

  Cecilia Östlund, et al., bioRxiv - Cell Biology 2022

  Quote: ... Protein depletion was confirmed by immunoblotting with specific Abs against lamin A/C (Santa Cruz Biotechnology, sc-20681) at a dilution of 1:3000 ...
• Hepatocytes deficient in nuclear envelope protein lamina-associated polypeptide 1 are an ideal mammalian system to study intranuclear lipid droplets

  Cecilia Östlund, et al., bioRxiv - Cell Biology 2022

  Quote: ... LAP1 (33) at a dilution of 1:5000 and 7-actin (Santa Cruz Biotechnology, sc-47778) at a dilution of 1:3,000.
• The nucleoporin Nup170 mediates subtelomeric gene silencing through the Ctf18-RFC complex and PCNA

  Sanjeev Kumar, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit polyclonal anti-Clb2 (Santa Cruz; sc-9071), and rabbit polyclonal anti-G-6-PDH (Sigma ...
• Machine learning-based classification of binary dynamic fluorescence signals reveals muscle stem cell fate transitions in response to pro-regenerative niche factors

  Matteo Togninalli, et al., bioRxiv - Cell Biology 2022

  Quote: ... Cells were subsequently incubated with primary antibodies against Pax7 (Developmental Studies Hybridoma Bank, 2 μg/mL) and MyoD (Santa Cruz Biotechnology, clone G-1, 1:200) diluted in 2.5% BSA and 2.5% GS in PBST ...
• Machine learning-based classification of binary dynamic fluorescence signals reveals muscle stem cell fate transitions in response to pro-regenerative niche factors

  Matteo Togninalli, et al., bioRxiv - Cell Biology 2022

  Quote: ... and then cells were stained with rabbit cleaved caspase-3 primary antibody (Santa Cruz Biotechnology, sc-7148, 1:200) and Alexa Fluor 647-conjugated goat anti-rabbit IgG secondary antibody (Jackson Immunoresearch Laboratories ...
• An anti-steatosis response regulated by oleic acid through lipid droplet-mediated ERAD enhancement

  Jorge Iván Castillo-Quan, et al., bioRxiv - Cell Biology 2022

  Quote: ... animals were treated with PBS containing 2% paraformaldehyde (PFA; Santa Cruz: SC-281692) then snap frozen in a dry ice/ethanol bath for at least 3 times to permeabilize the cuticle ...
• The inner nuclear membrane protein NEMP1 is required for nuclear envelope openings and enucleation of erythroblasts during erythropoiesis

  Didier Hodzic, et al., bioRxiv - Cell Biology 2022

  Quote: ... goat LaminB1 (Santa Cruz Biotechnology) were used for immunofluorescence microscopy ...
• Nuclear receptor interaction protein (NRIP) as a novel actin-binding protein involved in invadosome formation for myoblast fusion

  Hsin-Hsiung Chen, et al., bioRxiv - Cell Biology 2022

  Quote: ... and anti-GFP (Santa Cruz, 1:20)] at 4°C overnight ...
• On optimal temozolomide scheduling for slowly growing gliomas

  Berta Segura-Collar, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse anti-GAPDH (1:1.500, Santa Cruz Biotechnology #sc-47724), rabbit anti-pTyr1068-EGFR (1:1.000 ...
• The chromatin associated Sin3B is a critical regulator of DNA damage repair through the engagement of the Non-Homologous End Joining

  Jorge Morales-Valencia, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse anti-Lamin A/C (Santa Cruz sc-376248).
• Tumor-educated monocytes suppress T cells via adenosine and depletion of adenosine in the tumor microenvironment with adenosine deaminase enzyme promotes response to immunotherapy

  Omar S. Sarkar, et al., bioRxiv - Cell Biology 2022

  Quote: ... and from Santa Cruz Biotechnology ...
• FAK drives resistance to therapy in HPV-negative head and neck cancer in a p53-dependent manner

  Phillip M. Pifer, et al., bioRxiv - Cancer Biology 2022

  Quote: ... from Santa Cruz Biotechnology (Santa Cruz, CA). Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000 ...
• FAK drives resistance to therapy in HPV-negative head and neck cancer in a p53-dependent manner

  Phillip M. Pifer, et al., bioRxiv - Cancer Biology 2022

  Quote: ... β-actin (C4, 1:10,000, #MAB1501) from MilliporeSigma (Burlington, MA): and p53 (DO-1, 1:1000, #SC-126) from Santa Cruz Biotechnology (Santa Cruz ...
• Defining the Ligand-dependent Interactome of the Sigma 1 Receptor

  Jing Zhao, et al., bioRxiv - Cell Biology 2022

  Quote: ... Sec61alpha (Santa Cruz; 1:100 for western); Sigma1 Receptor (1:500 for western ...
• Defining the Ligand-dependent Interactome of the Sigma 1 Receptor

  Jing Zhao, et al., bioRxiv - Cell Biology 2022

  Quote: ... the membrane was incubated for 1h using an appropriate Horseradish Peroxidase (HRP)-conjugated secondary antibody (Santa Cruz Biotechnology) at room temperature ...
• A CRISPR screen identifies redox vulnerabilities for KEAP1/NRF2 mutant non-small cell lung cancer

  Chang Jiang, et al., bioRxiv - Cancer Biology 2022

  Quote: ... α-tubulin (Santa Cruz, TU-02, Cat #sc-8035), SOD2 (Cell Signaling Technologies ...
• Blockade of redox second messengers inhibits JAK/STAT and MEK/ERK signaling sensitizing FLT3-mutant acute myeloid leukemia to targeted therapies

  Zacary P. Germon, et al., bioRxiv - Cancer Biology 2022

  Quote: ... p22phox (Santa Cruz Biotechnology, Dallas, TX) p47phox ...
• A CRISPR screen identifies redox vulnerabilities for KEAP1/NRF2 mutant non-small cell lung cancer

  Chang Jiang, et al., bioRxiv - Cancer Biology 2022

  Quote: ... GSR (Santa Cruz Biotechnology, Cat #sc-133245), TRXR1 (Cell Signaling Technologies,15140S) ...
• Activation of targetable inflammatory immune signaling is seen in Myelodysplastic Syndromes with SF3B1 mutations

  Gaurav S Choudhary, et al., bioRxiv - Cancer Biology 2022

  Quote: ... IRAK1 (sc-7883; Santa Cruz), phospho-IRAK1 (T209 ...
• THE STABILIZATAION OF C-MYC BY THE NOVEL CELL CYCLE REGULATOR, SPY1, DECREASES EFFICACY OF BREAST CANCER TREATMENTS

  Rosa-Maria Ferraiuolo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... followed by the use of 1 mg/ml polybrene (Santa Cruz Biotechnology) and MOI 3 of the specific vector used ...
• EZH2 endorses cell plasticity to carcinoma cells facilitating mesenchymal to epithelial transition and tumour colonization

  Amador Gallardo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse anti-TUBULIN (Santa Cruz). Secondary species-specific antibodies conjugated to horseradish peroxidase were used ...
• THE STABILIZATAION OF C-MYC BY THE NOVEL CELL CYCLE REGULATOR, SPY1, DECREASES EFFICACY OF BREAST CANCER TREATMENTS

  Rosa-Maria Ferraiuolo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... anti-phospho-c-Myc-T58 (Santa Cruz Biotechnology; 1:1000), Cyclin E1 (Abcam ...
• THE STABILIZATAION OF C-MYC BY THE NOVEL CELL CYCLE REGULATOR, SPY1, DECREASES EFFICACY OF BREAST CANCER TREATMENTS

  Rosa-Maria Ferraiuolo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and anti-ERK1/2 (Santa Cruz Biotechnology; 1:1000). Secondary antibodies were used at 1:10000 dilution in blocker for 1 hour at room temperature ...