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Citations for Santa Cruz :
651 - 700 of 718 citations for Recombinant Swine CCL19 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and identified by immunoblotting with a mouse anti-green fluorescent protein (GFP) (Santa Cruz Biotechnology, Santa Cruz, CA), or anti-Atg13 [175] antibody to detect tagged or endogenous proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1000, catalog no. sc-9996, Santa Cruz Bio-technology) and rabbit polyclonal anti-SYT1 antibody (1:5000) ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653) in the dark at room temperature for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... at 1:10,000 dilution and the lower portion was incubated with a mouse monoclonal antibody against histone H3 protein (Santa Cruz Biotechnologies #sc-517576) at 1:5,000 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The epitope-tagged Pum and α-tubulin proteins were detected by successive incubation with anti-Myc 9E10 monoclonal primary (SC-40, Santa Cruz Biotechnology) and anti-α-tubulin B-5-1-2 monoclonal primary (T5168 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining supernatant was subjected to immunoprecipitation using 15 μl of mouse monoclonal antibody against green fluorescent protein (catalog no. sc-9996, Santa Cruz, CA, USA). The immunoprecipitate was gently shaken overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Nucleus proteins of the ANKRD49-OE or LV5 H1299 cells (1×106) were extracted and 25 µg of them were precleared using Protein A/G Agarose (Santa Cruz, California, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was processed for DREADDs expression validation and selected tissue was stained for antibody against striatal-enriched protein tyrosine phosphatase (STEP, mouse, dilution 1:500, Santa Cruz sc-23892).
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FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5bioRxiv - Neuroscience 2024Quote: ... beads were resuspended in Laemmli sample buffer and protein were eluted by boiling 10 minutes at 90°C and analyzed by SDS-PAGE and immunoblotting using FUS (Santa Cruz sc-47711), ETV5 (Proteintech 13011-1-AP ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: LDLR cell surface protein was quantified by fixing cells in 4% paraformaldehyde and incubated with anti-LDLR at 1:100 (Santa Cruz Biotechnology, sc18823) for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Co-IP was performed using anti-PNKP (BioBharati Life Science, BB-AB0105) antibody with Protein A/G PLUS agarose beads (Santa Cruz Biotechnology, sc2003,) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 500 μg of the supernatant proteins were incubated with 2 μg of mouse monoclonal antibody raised against SNAP-25 (Santa Cruz, sc-390814) or Syntaxin1a (MilliporeSigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Separated proteins were transferred to a PVDF membrane and the membrane was incubated with antibodies to the HA epitope tag (Santa Cruz Biotechnology, sc-805) and α tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with the antibodies for 1h at 4°C on a roller before 80µl Protein G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA) were added and samples were incubated overnight at 4°C on a roller ...
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bioRxiv - Synthetic Biology 2021Quote: ... Blots were then washed three times for 15 minutes at 4 °C with 0.05% TBS-Tween and stained for 4 hours with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) (Santa Cruz Biotech, sc-516102) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4, SC-47778, mouse monoclonal antibody, Santa Cruz Biotechnology, USA), anti-GFP (B-2 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibodies to cellular proteins were used as markers for early endosomes (EEA-1; rabbit polyclonal antibody; Santa Cruz Biotechnology Inc; 1:200), and the trans-Golgi (TGN46 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins extracts (1.5 mg) of embryos expressing CG11412-myc were incubated with 1 μg of c-Myc antibody (Santa Cruz, sc-40, clone 9E10) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the HIS-tagged AtCFI25a protein bound to GST-AtCFI59 was detected by western blot analysis using an anti-HIS antibody (G18) (sc-804; Santa Cruz Biotechnology, Dallas, USA) at 1:5000 dilution.
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bioRxiv - Cell Biology 2023Quote: ... 50 µg of protein extract was incubated at 37°C for 1 hr with the artificial p-nitrocatecholsulfate substrate (Santa Cruz Biotechnology, sc-238927A) and either sodium pyrophosphate (ARSA ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were loaded per lane and blots were probed with anti-mGFP antibodies (Santa Cruz Biotech, #sc-9996; 1:2000 dilution), anti-elongation factor 1-α (EF1A ...
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bioRxiv - Molecular Biology 2022Quote: ... The remained liquids were added with anti-NS1 or -VP2 or -IgG antibodies and incubated at 4°C for 4 h followed by addition of protein A/G PLUS-Agarose (Santa Cruz Biotech, sc-2003) and incubation overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to each sample and then incubated at 4°C for 4 hours followed by addition of protein A/G PLUS -Agarose (Santa Cruz Biotech, sc-2003) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were immunoprecipitated by incubation with protein A/G magnetic beads (HY-K0202, MCE) and anti-GFP antibody (sc-9996, Santa Cruz Biotechnology; 1:50) at 4 °C overnight with gentle rocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated with primary antibody or rabbit IgG (control) for 3hr at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 35min with head-to-tail rotation either in the absence or presence of 100 µM CCT blocking peptide SAGRRFIVSPVPE (United Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated with primary antibody (control sample incubated with rabbit IgG primary antibody: 1:100) overnight at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 1 hour with head-to-tail rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitation was performed as previously described (3) and the bound proteins were analyzed by western blotting using the following antibodies: anti-AnxA6 (Santa Cruz Biotechnology sc-271859), anti-β-actin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... cultured cells were homogenized in IP cell lysis buffer (Beyotime) and incubated with primary antibody and Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... After each chase period the cells were lysed in RIPA buffer and XPO1 protein was recovered from whole lysates by immunoprecipitation using mouse monoclonal XPO1 antibody (Santa Cruz Biotech, sc-5595) and subjected to SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: Protein expression was quantified by western blot analysis using the following antibodies: anti-cyclin A (1:500 sc-751; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Histone H3 (phospho Serine10 ...
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bioRxiv - Cell Biology 2022Quote: ... or mouse brain homogenates (1 mg) were pre-cleared with 30 µL of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...