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Citations for Santa Cruz :
651 - 700 of 715 citations for Galectin 3 LGALS3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... For knockdown of HLTF or FBH1, pools of 3 target-specific siRNAs (sc-45943 and sc-90462, respectively) were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... and p-NF-kB levels the following antibodies were used: mouse anti-Caspase-3 (1:400, sc-56053 Santa Cruz Biotechnology), rabbit anti-p-ERK1/2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded in microslide channels at a final concentration of ∼5×105 cells/ml in 120 µL cell culture media containing 40 μM BrdU:BrdC mix (3:1, Cat.# B5002, Sigma and Cat.# 284555, Santa Cruz Biotec) and grown for 17-24 h ...
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bioRxiv - Biochemistry 2023Quote: ... The detector antibody (biotinylated CD9, CD63, CD81 antibodies or or CD42a and CD62P by MiltenyBiotech or anti-band 3 from Santa Cruz) is used at the concentration of 0.6 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The following assay positive controls were used at a starting concentration of 1 µg/ml: (1) Anti-ASC/TMS1/PYCARD Antibody (B-3) mouse monoclonal IgG1 (sc-514414, Santa Cruz); (2 ...
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bioRxiv - Cancer Biology 2023Quote: ... β-actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression were determined using mouse antibodies sc-47724 and sc-47778 (Santa Cruz) (1:2500 ...
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bioRxiv - Cell Biology 2022Quote: ... ChIP for human endogenous GATA4 in SH-SY5Y cells was carried out using the GATA-4 (G-4) (Cat. sc-25310, Santa Cruz, 1:50, RRID:AB_627667). All primers for qRT-PCR or ChIP-qPCR are as indicated in Table S3.
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bioRxiv - Biophysics 2020Quote: ... the membrane was washed 3 times with PBST and incubated with an anti-Mouse HRP conjugated secondary antibody (Santa Cruz, sc-2005) diluted 1 ...
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... eluted protein complexes were diluted in delivered sample buffer and resolved on 8% SDS-PAGE gels followed by Western blot analysis and immuno-detection with ARNT 1 antibody (mouse monoclonal, sc-17812, G-3, Santa Cruz Biotechnology). Chemiluminescent detection was performed using horseradish peroxidase-conjugated anti-mouse secondary antibody (7076S ...
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bioRxiv - Developmental Biology 2021Quote: ... the blots were incubated for one hour at room temperature with the secondary antibody dissolved in TBS-T containing 3% semi-skimmed milk (rabbit anti-goat IgG-HRP, 1:2,500, Santa Cruz sc-2768). The blots were then washed as above ...
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bioRxiv - Molecular Biology 2021Quote: ... 3% BSA) for 30min on rotating wheel at room temperature and successively incubated with CTNND1 e2 primary antibody (Santa Cruz sc-23873) for 1h30 on rotating wheel at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The anti-Bid mAb (B-3) for EMSAs and anti-β-actin mAb (DM1A) used in western blotting experiments were also purchased from Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked with 5% BSA/TBST for 3 h at room temperature and incubated with anti-β-actin (Santa Cruz Biotechnology, sc-47778), anti-cortactin (Abcam ...
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bioRxiv - Immunology 2022Quote: ... 80% confluent HEK293-T cells were transfected with 2 μg of TCR plasmid and 1 μg pCL-Eco helper plasmid with 6 μg (3× DNA mass) PEI (Santa Cruz Biotech) to create retrovirus ...
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated in primary antibody solution (3% BSA in PBS-T) with 1 μg/mL of both α-RELA (sc-8008; Santa Cruz) and NEMO (sc-8330 ...
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bioRxiv - Physiology 2021Quote: ... adenovirus was added to the medium for 3 hours on day 4 of differentiation at 250 MOI in the presence of 5 μg/ml polybrene (Santa Cruz Biotechnology). Experiments were performed 3 days later ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was washed with TBST (5min × 3) and then incubated in m-IgGκ BP-HRP secondary antibody (Santa Cruz, sc-516102) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... with the following primary antibodies diluted in blocking solution (3% nonfat milk): goat polyclonal anti-lamin A/C (1:500; sc-6215 Santa Cruz Biotechnology); mouse monoclonal anti-lamin A/C (1:500 ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used for cell staining and zebrafish were CtBP [CtBP (B-3, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA)] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Positive convalescent serum (heat-inactivated at 56°C for 30 min) of a COVID-19 patient and anti-human IgG (Santa Cruz Biotechnology, Dallas, TX, USA) were used for a viral inhibition as positive control and negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated with the primary antibodies overnight at 4°C in TBST and 3% BSA. The primary antibodies used in this study are anti-(α) human CHI3L1 (R&D. AF2599-SP) and α-KEAP-1(Santa Cruz Biotechnology. 8047), β-actin (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2021Quote: ... The primary antibodies used for incubation were anti-TGFβ 1/2/3 [H-112] (dilution 1: 80, Santa Cruz Biotechnology, Inc., sc-7892), anti-COX2 antibody [EP1978Y] (dilution 1 ...
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bioRxiv - Neuroscience 2019Quote: ... permeabilized in PBS with 10% triton-x for 30 min and incubated for three days at 4 °C with goat anti-DCX antibody (1:250 in 10% triton-x and 3% horse serum (sc-8066; Santa Cruz Biotechnology, USA). Sections were washed and incubated in biotinylated donkey anti-goat secondary antibody for 60 minutes (1:250 ...
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bioRxiv - Immunology 2020Quote: ... 1xPBS) and incubated overnight at 4°C using the following dilutions: anti-COPA (1:100, Santa Cruz Biotechnology, clone H-3, sc-398099) and anti-STING (1:100 ...
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bioRxiv - Genetics 2019Quote: ... USA) for 24 hours at 4°C or rabbit anti glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) (1:1000, Santa Cruz, Dallas, TX, USA) for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The blots were washed 3 times in TBS with 0.1% Tween-20 before being incubated with either rabbit (Santa Cruz Biotechnology, SC-2004) or mouse (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2019Quote: ... Then the blot was washed 3 times with PBST and probed with secondary anti-mouse conjugated with HRP (Santa Cruz Biotechnology: sc-2005) at 1:4500 dilution for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The results of the densitometric analysis of the bands were expressed as optical density (O.D.) units relative to the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Santa Cruz Biotechnology Inc., USA) [20].
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 3 times with PBS and incubated with 1:400 secondary antibody donkey IgG anti–goat CFL 488 (SC362255, Santa Cruz Biotechnology, USA) for 1 h at RT ...
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bioRxiv - Genetics 2022Quote: ... Once the cells were ∼50% confluent (after 2-3 days) the media was changed to StemFlex with 1ug/uL polybrene (Santa Cruz SC-134220), and 10uL each of Ngn2 and rtTA virus was added to each well ...
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Dynamic Effects of Ventral Hippocampal NRG3/ERBB4 Signaling on Nicotine Withdrawal-Induced ResponsesbioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature before reacting overnight at 4°C with primary antibodies: Neuregulin-3 (NRG3) (1:500, sc-67002, N-terminal extracellular domain, Santa Cruz Biotechnology, Santa Cruz, CA), ERBB4 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 washes with BB and incubation with anti-Tom20 antibodies conjugated with Alexa 647 fluorophore (Santa Cruz Biotechnology Inc.; 1:100) for 90 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by fluorescent staining with Pan-Cytokeratin (PanCK, clone AE-1/AE-3, AF488, Santa Cruz NBP2-33200AF488, [2 µg/mL], CA, USA) to identify trophoblasts ...
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bioRxiv - Neuroscience 2024Quote: ... Then 10% of the lysate was set aside for use as an input control and the rest was tumbled overnight at 4°C with 7 µl of ERα antibody or 1.4 µg of Homer antibody (Santa Cruz Biotechnology, D-3). The same isotype IgG was used as control for the specificity of the ERα (Abcam #ab172730 ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Cancer Biology 2020Quote: Sections from formalin fixed paraffin embedded mouse tissue or a human pancreatic cancer tissue microarray (Biomax PA961e) were stained with antibodies against PC (Santa Cruz sc-67021, 1:50 dilution) or ME1 (Proteintech 16619-1-AP ...
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bioRxiv - Immunology 2020Quote: ... Membranes were probed with primary anti-human nuclear factorkappa B (NF-κB) p65 (c-20) sc-372 (1:200 dilution, Santa Cruz Biotechnology, Palo Alto, CA, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared as described above and immunoprecipitated for 3 hrs at 4°C with the following antibodies: mouse anti-Myc (9E10, Santa Cruz Biotechnology, 1 μg) or mouse anti-Flag (M2 ...
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bioRxiv - Neuroscience 2021Quote: ... with TBS-T and primary antibodies were detected after incubation (3 h) with either rabbit anti-goat IgG-AP (1:2000) or goat anti-rabbit IgG-AP (1:2000) (Santa Cruz, Dallas, TX, USA). Alkaline phosphatase activity was detected with BCIP/NBT AP-conjugate substrate reaction kit (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 × 10 min with TBST and incubated with the secondary antibody HRP-conjugated goat anti-mouse (1:10000, Santa Cruz Biotechnology #sc-2005) in 5% BLOTTO for 1 hr at RT and washed again 3 × 10 min with TBST ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then washed 3 × 5 min in TBS-T and incubated with 1:5000 goat anti-rabbit HRP secondary antibody (Santa Cruz Biotechnology, sc-2004) in 5% milk in TBS-T for 1 h followed by 3 × 5 min washes in TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was incubated with the following primary antibodies in TBST + 5% BSA at 4°C overnight: rabbit polyclonal anti-MEK-3 (1:500, Santa Cruz Biotechnology, #sc-961), rabbit polyclonal anti-p38 (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... 2° antibodies IgG-HRP anti-goat sc-2953 and anti-mouse sc-2314 at 1:1000 dilutions were incubated for 1 h in 3% milk (all antibodies through Santa Cruz Biotechnology, Dallas, US), followed by three 15 min washes with TBST ...
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bioRxiv - Neuroscience 2020Quote: ... placed in a blocking solution (0.5% TX-100 in PBS + 3% NDS) and then incubated for 2 days at 4°C in goat anti-calbindin (1:200; Cat#SC7691; Santa Cruz Biotechnology, Dallas, Texas), followed by 2.5 hours incubation at RT of secondary antibody coupled to a green fluorophore (1:200 ...
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bioRxiv - Cell Biology 2020Quote: For the generation of conditioned media tumor B-cells were incubated with 3 µM of mafosfamide sodium salt (Santa Cruz, Santa Cruz, CA, USA), 10 µM Nutlin-3A (Tocris Bioscience ...
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bioRxiv - Cell Biology 2020Quote: For the generation of conditioned media tumor B-cells were incubated with 3 µM of mafosfamide sodium salt (Santa Cruz, Santa Cruz, CA, USA), 10 µM Nutlin-3A (Tocris Bioscience ...