3 3 2 3 Dimethyl indol 1 yl 2 hydroxy propylamino propan 1 ol citations for Santa Cruz

Quote: ... Briefly 3 x105 cells were incubated on ice with mouse anti-human ACE2 monoclonal antibody conjugated to AF-647 (Santa Cruz Biotechnology, USA, cat. SC-390851) following manufacturer’s instructions ...

ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genes

Jinhong Dong, et al., bioRxiv - Genomics 2024

Quote: ... was aliquoted into each of 3 tubes with antibody (2µg anti-HA Invitrogen #26183, 8µg anti-SP1 Santa Cruz Biotechnology sc-17824 X, or mock IP) and incubated with end-over-end rotation at 4°C overnight ...

Intratumoral Heterogeneity Promotes Collective Cancer Invasion Through NOTCH1 Variation

Peter Torab, et al., bioRxiv - Cancer Biology 2021

Quote: UM-UC-1 cells (2×105) cells were transfected with 2.5 mg of HNF-3alpha CRISPR/Cas9 KO plasmid (Santa Cruz; sc-400743) using Lipofectamine 3000 (Thermo Fisher Scientific) ...

Cdc42 activity is essential for the interplay between cAMP/PKA pathway and CatSper function

Guillermina M. Luque, et al., bioRxiv - Cell Biology 2020

Quote: ... Antibodies were diluted in 2% nonfat dry milk in T-PBS as follows: 1:500 for anti-Cdc42 (Santa Cruz sc-8401); 1:750 for anti-Cdc42 (Santa Cruz sc-87) ...

Sex differences in central inflammatory pain sensitization are associated with differential expression of glycine receptors and GLP-1 at the spinal cord

TA. Mariqueo, et al., bioRxiv - Physiology 2020

Quote: ... the membranes were washed three times in PBST and incubated 2 h with horseradish peroxidase (HRP) secondary antibodies (1:5000, Santa Cruz Biotechnology). This procedure was followed by three membrane washes with PBST ...

Arabidopsis Casein Kinase 2 triggers Stem Cell Exhaustion under Al Toxicity and Phosphate Deficiency Through activation of the DNA Damage Response pathway

Pengliang Wei, et al., bioRxiv - Plant Biology 2020

Quote: ... the membrane was incubated for 2 h at room temperature in the anti-Myc primary antibody A-14 (1:2000 dilution, lot no. F0810; Santa Cruz Biotechnology), rinsed three times with 1X TBST (0.01 M Tris-HCl ...

Nutrient copper signaling promotes protein turnover by allosteric activation of ubiquitin E2D conjugases

C.M. Opazo, et al., bioRxiv - Cell Biology 2021

Quote: ... Immunoprecipitation was performed by incubating samples in 2 g/mL anti-rabbit (FL-393, sc-6243) or anti-mouse (DO-1, sc-126) P53 antibodies (Santa Cruz Biotechnology) for 2 h at 4 °C ...

Nuclear lamina strain states revealed by intermolecular force biosensor

Brooke E. Danielsson, et al., bioRxiv - Biophysics 2022

Quote: ... the immunoblots were blocked with 2% BSA and incubated with primary antibodies: Anti-Lamin A/C antibody (E-1) (sc-376248, Santa Cruz Biotechnology) and Anti-Actin antibody ...

Use of a novel knock-in allele of Pkd1 identifies nicotinamide nucleotide dehydrogenase as a mitochondrial binding partner of polycystin-1

Cheng-Chao Lin, et al., bioRxiv - Genetics 2022

Quote: ... anti-Polycystin-2 (PC2-CT 27, PKD-RCC (https://www.pkd-rrc.org/) and anti-Polycystin-1 (7E12, Santa Cruz cat. no. sc-130554) and secondary labeled antibodies (Licor ...

Social memory in female mice is rapidly modulated by 17β-estradiol through ERK and Akt modulation of synapse formation

Paul A. S. Sheppard, et al., bioRxiv - Neuroscience 2022

Quote: ... or 10ng/side of PI3K inhibitor 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002; Santa Cruz Biotechnology, Dallas, TX); or vehicle (50% dimethyl sulfoxide [DMSO] in 0.9% NaCl solution ...

Profiling of Nascent Lariat Intermediates Reveals Key Genetic Determinants of the Timing of Human Co-transcriptional Splicing

Yi Zeng, et al., bioRxiv - Molecular Biology 2021

Quote: K562 cells at 90% confluency were treated for 2 hours with one of the following reagents (1) pladienolide B (Santa Cruz Biotechnology) in DMSO to a final concentration of 10 μM or (2 ...

Photoreceptor Compartment-Specific TULP1 Interactomes

Lindsey A. Ebke, et al., bioRxiv - Cell Biology 2021

Quote: ... Slides were then incubated with blocking solution (1% BSA and 10% Donkey serum in freshly prepared 1X PBS) for 2 hrs at RT and then subsequently incubated with primary antibodies: MAP1B at 1:100 (N-19, Santa Cruz Biotechnology), Kif3a at 1:300 (Abcam ...

IL8 Drives an Elaborate Signal Transduction Process for CD38 to Produce NAADP from NAAD and NADP⁺ in Endolysosomes to Effect Cell Migration

Tae-Sik Nam, et al., bioRxiv - Cell Biology 2020

Quote: ... containing 5 % nonfat dry milk for 2 h at room temperature and then incubated with anti-NaK-ATPase (1:2500; Santa Cruz Biotechnology), anti-LAMP-1 (1:2500 ...

Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord

Yuka Nakamura, et al., bioRxiv - Neuroscience 2022

Quote: The sections were then washed with PBS and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Santa Cruz Biotechnology) to visualize nuclei ...

SLC3A2 N-glycosylation and alternate evolutionary trajectories for amino acid metabolism

Cunjie Zhang, et al., bioRxiv - Cell Biology 2022

Quote: ... Cells lysates in Tris pH 7.5 50mM and 2% SDS were applied to SDS-PAGE followed by Western blotting using anti-SLC3A2 (Santa Cruz, 1:5000) and anti-ϒ-tubulin antibodies (1:10,000) ...

RUNX1 is regulated by androgen receptor to promote cancer stem markers and chemotherapy resistance in triple negative breast cancer

Natalia B Fernández, et al., bioRxiv - Cancer Biology 2022

Quote: ... membranes were incubated overnight at 4 °C with primary antibodies in 0.5% BSA or 2% non-fat milk in T-TBS: anti-AR (1:1000 dilution; Santa Cruz Biotechnology #7305), anti-RUNX1 (1:1000 dilution ...

Cleavage of cFLIP restrains cell death during viral infection and tissue injury and favors tissue repair

Kristel Martinez Lagunas, et al., bioRxiv - Cell Biology 2022

Quote: ... the sections were stained for the following antibodies (diluted in 0.2% (v/v) Triton X-100 in Animal Free Blocker and Diluent) overnight at 4°C: CC10 (1:50, Santa Cruz-sc365992), α-smooth muscle actin-Cy3 conjugated (1:50 ...

Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina

Elina Mäntylä, et al., bioRxiv - Molecular Biology 2023

Quote: ... and lamin A/C N-terminus residues aa 2-29 (LA/C-N, [E-1], sc-376248, Santa Cruz Biotechnology, DA, USA), a rabbit monoclonal Ab (rMAb ...

Activation of STING due to COPI-deficiency

Annemarie Steiner, et al., bioRxiv - Immunology 2020

Quote: ... Membranes were probed with primary antibody diluted in 5% skim milk/TBST or 5% Bovine Serum Albumin (BSA)/TBST over night at 4°C: anti-COPA (Santa Cruz Biotechnology, clone H-3, sc-398099), anti-phospho-STAT1 Tyr701 (Cell Signaling Technology ...

Combinatorial mathematical modelling approaches to interrogate rear retraction dynamics in 3D cell migration

Joseph H.R. Hetmanski, et al., bioRxiv - Cell Biology 2020

Quote: ... and a Ect-2 antibody (Santa Cruz) and incubated with appropriate secondary ...

B cell-intrinsic requirement for WNK1 kinase in T cell-dependent antibody responses

Darryl Hayward, et al., bioRxiv - Immunology 2021

Quote: ... anti-ERK2 (D-2, Santa Cruz Biotechnology), anti-MYC (Y69 ...

Cardiomyocyte differentiation from iPS cells is delayed following knockout of Bcl-2

Tim Vervliet, et al., bioRxiv - Developmental Biology 2022

Quote: ... anti-phospho Ser70-Bcl-2 (Santa Cruz), anti-phospho-p38MAPK ...

Filopodia powered by class X myosin promote fusion of mammalian myoblasts

David W. Hammers, et al., bioRxiv - Cell Biology 2021

Quote: ... anti-Myo10 (2 µg/mL; Santa Cruz Biotechnology No ...

KIAA1841, a novel SANT and BTB domain-containing protein, inhibits class switch recombination

Simin Zheng, et al., bioRxiv - Molecular Biology 2020

Quote: ... anti-GFP-HRP (B-2, Santa Cruz), anti-Flag (M2 ...

Integrative Analysis the characterization of peroxiredoxins in pan-cancer

Lei Gao, et al., bioRxiv - Cancer Biology 2020

Quote: ... BCL-2 (Santa Cruz, #sc-20067, CA), JAK2 (Santa Cruz ...

Local and substrate-specific S-palmitoylation determines subcellular localization of Gαo

Gonzalo P. Solis, et al., bioRxiv - Cell Biology 2022

Quote: ... 2-bromopalmitate (sc-251714) from Santa Cruz Biotechnology ...

Subcellular relocalization and nuclear redistribution of the RNA methyltransferases TRMT1 and TRMT1L upon neuronal activation

Nicky Jonkhout, et al., bioRxiv - Molecular Biology 2020

Quote: ... 2-(dimethylamino)guanosine (Santa Cruz, sc-220667). Data processing of chromatograms was performed using the Quanbrowser function of the Xcalibur Software package version 2.0.7 (Thermo Fisher Scientific) ...

S. Typhimurium impairs glycolysis-mediated acidification of phagosomes to evade macrophage defense

Saray Gutiérrez, et al., bioRxiv - Microbiology 2021

Quote: ... HKX-2 (sc-6521, Santa Cruz Biotechnology), v-ATPase A1 (V0 subunit ...

Computer Designed PRC2 Inhibitor, EBdCas9, Reveals Functional TATA boxes in Distal Promoter Regions

Shiri Levy, et al., bioRxiv - Molecular Biology 2020

Quote: ... Minocycline hydrochloride (2 μM; Santa Cruz Biotechnology) and IWR-endo-1 (0.5-1 μM ...

The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics

Astrid Kollewe, et al., bioRxiv - Biochemistry 2021

Quote: ... anti-ßArrestin 2 (sc-13140, Santa Cruz), anti-TRPC1 (4921 ...

The unfolded protein response of the endoplasmic reticulum protects Caenorhabditis elegans against DNA damage caused by stalled replication forks

Jiaming Xu, Brendil Sabatino, Stefan Taubert, bioRxiv - Genetics 2023

Quote: ... 2 mM IPTG (Santa Cruz sc-202185B), and 12.5 μg/mL tetracycline (BioBasic TB0504) ...