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Citations for Miltenyi Biotec :
101 - 150 of 181 citations for Urotensin II Related Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Naïve CD4 T cells were isolated using the Naïve CD4 T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: 3D imaging was performed with a light sheet fluorescence microscope (Ultramicroscope II; Miltenyi Biotec, Bergisch Gladbach, Germany) using Imspector Pro software (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... Myelin was removed by Myelin Removal Beads II (cat # 130-096-733, MACS Miltenyi Biotec, Auburn, CA) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Three-dimensional images following tissue clearing were acquired with a light-sheet microscope UltraMicroscope II (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: ... untouched Pan T cells were enriched by negative selection using T cell isolation kit II (Miltenyi biotec). All donor tissue information is included in Table S1.
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bioRxiv - Neuroscience 2024Quote: ... an additional myelin removal step was performed using Myelin Removal Beads II (#130-096-731, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were then isolated with Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Immunology 2021Quote: ... pDCs were further purified by magnetic sorting with human Diamond Plasmacytoid dendritic-cell isolation kit II (Miltenyi Biotec). Purity of cell preparation was assessed by flow cytometry and for all donors was more than 90%.
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Brain samples were imaged using a light sheet fluorescence microscope (UltraMicroscope II Light Sheet Microscope, Miltenyi Biotec, Germany) equipped with an sCMOS camera (Andor Neo) ...
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bioRxiv - Immunology 2020Quote: ... splenic T-cells from donor mice were enriched using the Pan T-cell Isolation Kit II (Miltenyi Biotec), while bone marrow (BM ...
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bioRxiv - Cancer Biology 2020Quote: ... MACS-based purification was performed with Myelin Removal Beads II followed by Mouse Cell Depletion kit (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... was used at 1:200 iDisco-processed hemibrains were imaged by light sheet microscopy (Miltenyi Biotec Ultramicroscope II) using their 4x objective ...
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bioRxiv - Cell Biology 2021Quote: Single plane illumination (light sheet) image stacks were acquired using an Ultramicroscope II and Ultramicroscope Blaze (Miltenyi BioTec). The available filter sets were ex 470/40 nm ...
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bioRxiv - Immunology 2021Quote: ... Isolated cells were negatively selected tandemly first with Myelin Removal Beads II (Miltenyi Biotec, Cat# 130-096-731) and second with anti-CD11b MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... pDCs were enriched from freshly isolated PBMCs using the human Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec) following manufacturer’s instructions with a 10% reduction in the recommended amounts of reagents ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD8+ T cells were purified from the spleen of OT-I.Ly5.1 mice using CD8a+ T Cell Isolation Kit II (Miltenyi Biotec). OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in RPMI 1640 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Neuroscience 2023Quote: ... enzyme mix II (enzyme A and buffer Y, from the Adult Brain Dissociation Kit #130-107-677, Miltenyi Biotec, mixed according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Cell suspension underwent two negative selection steps to deplete myelin (Myelin removal beads II, 130-096-733, Miltenyi Biotec) and CD45-positive cells (CD45 microbeads ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting cell suspension was washed twice with PBS and used for the isolation of naïve CD4+ T cells according to the manufacturer’s instructions (Naive T Cell Isolation Kit II; Miltenyi Biotec). Naive CD4+ T cell purity was assessed by staining for CD4 PerCPeFluor710 (clone ...
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bioRxiv - Immunology 2021Quote: ... Allogenic naïve CD4+ T cells and CD8+ T cells were isolated from buffycoats using the naïve CD4+ T cell Isolation kit II (Miltenyi Biotec) and CD8+ T cell Isolation kit (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...
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bioRxiv - Cell Biology 2019Quote: ... Monocyte-derived dendritic cells (MDDCs) were generated from plastic adherent or directly isolated monocytes (Monocyte Isolation Kit II, Miltenyi Biotec) through culture for 6 days in Roswell Park Memorial Institute medium (RPMI-1640 ...
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bioRxiv - Immunology 2019Quote: ... and T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Naïve antigen presenting cells were isolated from an unimmunized (naïve ...
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bioRxiv - Neuroscience 2022Quote: The brain stained for TSPO and imaged by NiR-OPT was consequently rescanned using an UltraMicroscope II (Miltenyi Biotec, Germany) including a 1x Olympus objective (Olympus PLAPO 2XC ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Immunology 2021Quote: ... NK cells in the spleen were isolated and purified by using mouse NK cell isolation kit II (130-096-892, Miltenyi Biotec). The purity of NK cells was analyzed by FACS ...
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bioRxiv - Immunology 2021Quote: ... and purified their T cells using the untouched isolation kit (Pan T Cell Isolation Kit II, mouse, 130-095-130, Miltenyi Biotec).
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bioRxiv - Pathology 2019Quote: ... Increased ratios of MDSCs and nonMDSCs were co-cultured with splenic T cells isolated with Pan T Cell Isolation Kit II (Miltenyi Biotec) and stained with 1 μM Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Microbiology 2021Quote: ... were pooled and dissociation was done using a collagenase I and II and a DNase I enzyme mix (all from BioConcept) and the gentleMACS Octo Dissociator (Miltenyi Biotec). Following this mechanical and enzymatical dissociation ...
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bioRxiv - Microbiology 2022Quote: ... hematocrit and at least 5% parasitaemia was enriched for trophozoites by passing through a MACS CS column placed in the magnetic field of a SuperMACS II (Miltenyi Biotec) separator according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... of cytapheresis rings from healthy donors (EFS Bordeaux) and B cells were sorted with the B cell isolation kit II (Miltenyi Biotec). Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... through the New York Blood Center and CD4+ cells isolated by negative selection with a Miltenyi CD4 T cell isolation kit II (Miltenyi Biotec). Cell-free virus was produced by transfection of 293T cells in 10 cm plates using polyjet (Signagen) ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... Supernatant was aspirated and the pellet was resuspended in 10μL Miltenyi Myelin Removal Beads II (Miltenyi Biotec Cat #: 130-096-733) and 90 μL MACS Buffer (0.5% BSA in HBSS w/o Ca2+/Mg2+ ...
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bioRxiv - Immunology 2020Quote: T cells were isolated from splenocytes of 8-12-week-old mice using a Pan T Cell Isolation Kit II (Miltenyi Biotec) with >90% purity ...
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bioRxiv - Neuroscience 2022Quote: ... with HBSS buffer with calcium and magnesium and prepared for myelin removal process (Myelin Removal Beads II, #130-096-733; Miltenyi Biotec). Then ...
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bioRxiv - Physiology 2019Quote: ... Naive CD4 T cells were isolated from PBMCs by negative selection using the human Naive CD4+ T Cell Isolation Kit II (Miltenyi Biotec). The isolation was >97% pure as assessed by flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...
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bioRxiv - Physiology 2020Quote: ... After MACS sorting of CD8+ T cell using a negative selection kit (CD8+ T Cell Isolation Kit II, human, Miltenyi Biotec), cells were lysed for western blotting or stained for Autophagy flux assay as described below ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...