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Citations for Miltenyi Biotec :
551 - 600 of 748 citations for Upstream stimulatory factor 2 USF2 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were incubated with APC-conjugated cytochrome c antibody (1: 50 dilution in permeabilisation buffer)(Miltenyi Biotec) and analyesd by FACS on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: For CD44 staining gliomaspheres were dissociated into single cells and stained with anti-CD44-APC antibody (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... PBMCs were depleted of CD14 mononuclear cells using anti-CD14 microbead antibodies (cat#130-050-201, Miltenyi Biotec) and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101 ...
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bioRxiv - Immunology 2024Quote: ... thymocytes were stained with anti-TCRδ-PE (GL3) antibody and enriched using anti-PE MicroBeads kit (Miltenyi Biotec). The exception was E17 thymus which did not undergo TCRδ enrichment ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Synthetic Biology 2021Quote: ... and MACSiBead Particles pre-coated with anti-human CD335 (NKp46) and CD2 antibodies based on manufacturer guidelines (Miltenyi Biotec). Primary adult CD34+ HSCs were cultured at 0.5 x 106 cells per mL in SFEMII medium supplemented with CC110 cytokine cocktail (StemCell).
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bioRxiv - Immunology 2021Quote: ... KIR2DL1 expressing cells were isolated by staining the entire NK cell population with anti-KIR1-PE antibody (Miltenyi Biotec) followed by magnetic separation using the EasySep™ human PE selection kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... Fluorochrome-conjugated antibodies were used for specific immunostainings with dilutions (in autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) as listed in table 4 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated with the surface antibody CD34-PE-Vio770 (clone AC136, lot #5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then collected and processed for intracellular staining with anti-IFN-γ-FITC monoclonal antibody (Miltenyi Biotec, Germany). Changes in the subset of lymphocyte cells and cytokine production (IFN-γ ...
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bioRxiv - Immunology 2019Quote: ... An antibody cocktail designed for negative selection of purified naïve T cells (Naïve Pan T cell purification kit, Miltenyi Biotec) was used together with extra αCD15/CD14 beads ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
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bioRxiv - Neuroscience 2023Quote: ... antibody labeling and boosting step was performed with the addition of CD11b (1:50) (Miltenyi Biotec, 130-110-611) and GFP-Nanobooster LOT 102 (1:500 ...
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bioRxiv - Genetics 2022Quote: ... The following antibodies were used for cell surface staining: IgA (clone IS11-8E10) (Miltenyi Biotec, cat#: 130-114-002), IgD (clone IA6-2 ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ donor splenocytes were enriched by CD45.1 negative selection using anti-mouse biotinylated CD45.1 antibody (clone A20, Ebioscience) and Anti-Biotin MicroBeads (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2023Quote: ... added with 40μL of magnetic microbeads conjugated with anti-Thy-1 antibody (Miltenyi Biotec 130–049–101, Auburn, CA), and mixed well ...
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bioRxiv - Neuroscience 2023Quote: ... with the latter two additionally analyzed by flow cytometry with suitable isotype antibodies (all: Miltenyi Biotec, Bergisch Gladbach, Germany) and unstained controls ...
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bioRxiv - Cancer Biology 2023Quote: ... To detect CD8, an antibody already labelled with PE-Vio-770 (130-113-721, clone REA734) from Miltenyi biotec was used at a concentration of 1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... while antibody rounds were either optically bleached or signal was removed using the REAlease® Release Reagent (Miltenyi Biotec). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Primary Anti-EpCAM APC conjugated antibody with isotype control was used for all experiments (Miltenyi Biotec, Surrey, United Kingdom). Cells were blocked in 3% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Collected PBMCs are resuspended with MACS buffer and appropriate antibodies conjugated with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequent magnetic cell separation proceeded with a column in the magnetic field followed by a collection of unlabeled cells ...
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bioRxiv - Bioengineering 2023Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Genetics 2023Quote: ... and enriched for iPSC-RPE via magnetically activated cell sorting using anti-CD24 and anti-CD56 antibodies (Miltenyi Biotec). iPSC-RPE cells in the negative cell fraction were seeded on the desired surface pre-coated with Laminin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Spleen qPCR samples were prepared by depletion of CD19+ TCRβ+ cells using biotinylated antibodies and Streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207 ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ cells were isolated by MACS using biotinylated anti-CD14+ antibody and anti-biotin microbeads according to the manufacture’s protocol (Miltenyi Biotec). Monocytes were cultured in complete RPMI plus 20 ng/ml recombinant human M-CSF for 7 days to promote differentiation into hMDMs ...
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bioRxiv - Developmental Biology 2022Quote: ... then bipotent progenitors were positively selected using a biotinylated EpCAM antibody (clone G8.8, eBiosciences) and streptavidin-conjugated magnetic beads (Miltenyi Biotec). This generates a >95% pure preparation of e16.5 bipotent alveolar progenitors (Sftpc+ Rage+ cells) ...
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bioRxiv - Genomics 2020Quote: ... and CD138+ multiple myeloma cells isolated from bone marrow biopsies by positive selection on antibody-conjugated magnetic beads (Miltenyi Biotec), were obtained with I.R.B ...
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bioRxiv - Neuroscience 2021Quote: ... kit with papain and double cell isolation using magnetic coupled antibodies anti-CD11B (Microglia) according to the manufacturer’s protocol (Miltenyi Biotec) and as previously described27 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...