Labshake search
Citations for Miltenyi Biotec :
351 - 400 of 664 citations for U3 Small Nucleolar RNA Associated Protein 4 Homolog CIRH1A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
-
bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
-
bioRxiv - Immunology 2020Quote: ... and the remaining adherent monocytes were cultured for 48h in the presence of IL-4 (300 U/ml) and GM-CSF (450 U/ml) (both from Miltenyi Biotec, Madrid, Spain) in X-VIVO 15 (BioWhittaker ...
-
bioRxiv - Cancer Biology 2022Quote: Samples were processed immediately after surgery and temporarily stored for transport at 4°C in tissue storage solution (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously described ...
-
bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were selected with MicroBeads-conjugated CD11b antibody (clone M1/70.15.11.5; Miltenyi Biotec B.V ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer and incubated with CD31-APC antibody (Miltenyi Biotec) for 30 min at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... 1:5000 diluted anti-GFP-HRP and anti-FLAG-HRP antibodies (Miltenyi biotec, USA) were used for detection.
-
bioRxiv - Plant Biology 2021Quote: ... The chromatin extracts were isolated and anti-GFP/anti-HA antibodies (Miltenyi Biotec, Germany) was used to immunoprecipitate protein-DNA complexes (Kaufmann et al ...
-
bioRxiv - Immunology 2020Quote: ... we used a CD63 antibody-labeled exosomes isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).
-
bioRxiv - Microbiology 2021Quote: ... PBMCs were incubated with anti-human CD14 antibodies coupled to magnetic beads (Miltenyi Biotec) and subjected to magnetic separation using LS columns (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2019Quote: ... and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605, Miltenyi Biotec, Germany).
-
bioRxiv - Microbiology 2019Quote: ... CD14+ PBMCs were enriched using anti-CD14 antibody microbeads (Miltenyi Biotec #130-050-201), according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... PE CD158a/h (KIR2DL1/DS1) anti-Human antibody: 130-099-209 from Miltenyi Biotec. APC CD158b1/b2j (KIR2DL2/DL3/DS2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec, 130-120-709) for 1 h at room temperature in the dark in 0.75% saponin (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... antibody labeling and subsequent isolation using a magnetic anti-APC-Microbead Kit (Miltenyi Biotec). NALM6 cells were cultivated in RPMI 1640 medium (Biowest ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with antibodies against viability fixable dye (Miltenyi Biotec, #130-109-812), CD3 (Miltenyi Biotec ...
-
bioRxiv - Immunology 2023Quote: ... CD80-PE and HLA-DR-VioBlue antibodies and isotype controls were from Miltenyi Biotec. Recombinant CD40L protein ...
-
bioRxiv - Plant Biology 2023Quote: ... and then with antibody (1:5000 anti-His-HRP (130-092-785, Miltenyi Biotec) or anti-FLAG-HRP (Ab49763 ...
-
bioRxiv - Microbiology 2024Quote: ... by negative selection after staining with CD304-PE antibody (clone AD5-17F6, Miltenyi Biotec) in the presence of human FcR blocking reagent (Miltenyi Biotec ...
-
bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
-
bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
-
bioRxiv - Cell Biology 2022Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
-
bioRxiv - Cell Biology 2019Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
-
bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
-
bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
-
bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
-
bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
-
bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
-
bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
-
bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
-
bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
-
bioRxiv - Genetics 2021Quote: ... RNA samples were incubated with 100 μl of streptavidin-coated μMACS magnetic beads (Miltenyi Biotec, 130-074-101) for 90 minutes at 24°C under gentle agitations ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer and incubated with the CD31-APC antibody (Miltenyi Biotec) for 30 min at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated for 15 min with CD11b antibody conjugated magnetic beads (Miltenyi Biotec) before passage through a magnetic bead column (Quadro MACS Separator and LS columns ...
-
bioRxiv - Cancer Biology 2022Quote: ... The antibodies chosen for the staining were: anti-GFAP APC (130-124-040, Miltenyi Biotec) for astrocytes ...
-
bioRxiv - Immunology 2020Quote: ... ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4, Miltenyi Biotec, Germany). All flow cytometry analyses were assessed using the following antibody clones ...
-
bioRxiv - Cell Biology 2019Quote: ... Magnetic columns and antibody-conjugated magnetic beads were purchased from Miltenyi Biotec (San Diego, CA). Liberase TL Research Grade enzyme mix and DNase I used in lung digestion were purchased from Roche Applied Science (Pleasanton ...
-
bioRxiv - Immunology 2021Quote: ... CD14 cells were purified by anti-CD14 monoclonal antibody (mAb)-conjugated magnetic microbeads (Miltenyi Biotec) and then cultured for 6 days in RPMI 1640 medium (Life Technologies Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... The following antibodies were applied: anti-CD3-FITC (1:25, Miltenyi Biotec, #130-098-162), anti-CD14-FITC (1:25 ...
-
bioRxiv - Genomics 2020Quote: ... anti-human EPCAM antibody conjugated to PE (1:50 dilution, Miltenyi Biotec #130-091-253), and either Calcein-AM (1:200 dilution ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2023Quote: ... the third aliquot containing 1 µL of CD45-VioBlue antibody (#130-110-802, Miltenyi Biotec); and the fourth aliquot containing 1 µL each of CD11b-APC antibody and CD45-VioBlue antibody ...
-
bioRxiv - Neuroscience 2023Quote: ... the second aliquot containing 1 µL of CD11b-APC antibody (#130-113-793, Miltenyi Biotec); the third aliquot containing 1 µL of CD45-VioBlue antibody (#130-110-802 ...