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Citations for Miltenyi Biotec :
251 - 300 of 393 citations for Tissue Grinders since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec) and published protocols 50,51 ...
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bioRxiv - Neuroscience 2020Quote: ... Microglia were isolated from adult mouse brain using the multi tissue dissociation kit 1 (Miltenyi Biotec #130-110-201), followed by CD11b microglia isolation kit (Miltenyi Biotec #130-093-634 ...
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bioRxiv - Immunology 2021Quote: Tissues homogenates were prepared by homogenizing perfused lungs and brains using gentleMACS Octo Dissociator (Miltenyi BioTec, #130-096-427) in M tubes (#130-093-335 ...
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bioRxiv - Neuroscience 2022Quote: ... We used our previous established isolation protocol37 using gentleMACS™ with the Neural Tissue Dissociation Kit (Papain) (Miltenyi Biotec) and a final concentration of 45 mM actinomycin D (Act-D ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue pieces (up to 500 mg) were transferred into a gentleMACS C tube (130-096-334, Miltenyi Biotec) containing 1,950 μL of enzyme mix 1 (enzyme P and buffer Z) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were digested using the Neural Tissue Digest Kit-P in C-tubes as per manufactures directions (Miltenyi Biotec). Two brains were digested in each C-tube using program 37_ABDK on the GentleMACS™ OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... The remaining tissue was further minced with scissors and enzymatically dissociated using the Human Tumour Dissociation Kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Neuroscience 2022Quote: ... brain cells were isolated using a 30%/80% Percoll gradient after homogenizing tissue on a gentleMACS dissociator (Miltenyi Biotec). Single cell suspensions were then incubated with purified anti-CD16/32 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated using a neural tissue dissociation kit according to the manufacturer’s protocol (Miltenyi Biotec, Auburn, CA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... liver tissues were digested and dissociated on the gentleMACS Dissociator with the Mouse Liver Dissociation Kit (Miltenyi Biotec, 130105807). After digestion and dissociation ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue and digestion buffer were transferred to a gentleMACS C Tube (run protocol 37C_h_TDK_1) (Miltenyi Biotec, 130-096-334) and incubated at 37 °C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was subjected to the 37C_ABDK_01 protocol on the gentleMACS Octo-Dissociator with heaters (Miltenyi Biotec, #130-096-427) for dissociation ...
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bioRxiv - Immunology 2022Quote: Tissues homogenates were prepared by homogenizing perfused lung and nasal turbinates using gentleMACS dissociator (Miltenyi BioTec, #130-096-427) with program RNA_02 in M tubes (#130-096-335 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The tumor samples were dissociated into single cells using GentleMACS tissue dissociator and its kit (Miltenyi Biotec, Gladbach, Germany). The tumor samples were cut into small pieces ...
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bioRxiv - Immunology 2023Quote: ... Liver tissue was dissociated by following the ‘preparation of single cell suspensions from mouse liver’ protocol from Miltenyi Biotec. Cells were then washed in cold PBS+ 2% FBS buffer before staining ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was subjected to the 37C_ABDK_01 protocol on the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec, #130-096-427). Lysate was filtered through a 70µm nyclon cell-strainer with Dulbecco’s PBS with calcium ...
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bioRxiv - Neuroscience 2023Quote: ... individual organoids were dissociated using the reagents from the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, #130-092-628).
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bioRxiv - Physiology 2023Quote: ... fresh mouse lung tissues were collected and dissociated using a lung dissociation kit (Miltenyi Biotec Inc., Auburn, CA, USA). Lung ECs were then isolated using two step magnetic bead separation (CD45 and CD31 antibody- conjugated microbeads ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspensions of the clinic tumor tissues mentioned above were prepared by Tumor Dissociation Kit (Miltenyi Biotec).
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec). Digestion was stopped with 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: Frozen tissue pieces were quickly thawed in a water bath before transferring into a GentleMACS dissociation tube (Miltenyi Biotec) containing Advanced DMEM/F12 + 1:100 Glutamax (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... The cerebrum was then cut into 16 pieces and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The mechanical dissociation of brain tissue was performed using the Adult Brain Dissociation Kit (Miltenyi Biotec #130-107-677) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The wound tissues underwent enzymatic epidermal-dermal separation using the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) followed by dissociation of the epidermis and dermis parts ...
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bioRxiv - Developmental Biology 2020Quote: Day 22 EMLOs (~25 count) were pooled and dissociated using the Neural Tissue Dissociation Kit (P, palpain) according to manufacturer’s instructions (Miltenyi Biotec) and similarly to previous reports44 ...
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bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802), following manufacturer’s instructions for manual dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspension was obtained by automated dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat#130-092-628) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... The perfused brains were dissected and cut into small 1 mm3 pieces before homogenizing them in DPBS++ containing 0.4% DnaseI on the tissue dissociator (Miltenyi Biotec) at 37 °C for 35 mins ...
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bioRxiv - Cell Biology 2021Quote: ... mice were euthanized and spleens were removed and mechanically disrupted with a GentleMacs tissue dissociator (Miltenyi Biotec Inc, Auburn, CA). Total spleen cells were washed with sterile PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The iPSC lines were cultured on tissue culture plastic coated with vitronectin (0.5 µg/cm2) in StemMACS iPS-Brew XF (Miltenyi Biotec). Pluripotency was validated by three lineage differentiation potential and Epi-Pluri-Score analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Cell Biology 2022Quote: ... Fresh tumor tissue from NSCLC patients were enzymatically digested and single-cell suspensions were stained with CD14 microbeads (Miltenyi Biotec) and positively selected using AutoMACS ProSeparator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: Mycobacterial loads in lung and spleen of euthanized mice were evaluated at different time points post infection by homogenizing the organs in PBS or PBS with 0.05 % Tween-80 using MACS tissue dissociator (Miltenyi Biotec). Guinea pigs were euthanized with an intra-peritoneal overdose of pentobarbital (>120 mg/kg) ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Up to 1.25 grams of cortical tissue were placed in each gentleMACS™ C Tube (Miltenyi Biotec, # 130-093-237) which contained 5 mL of 0.3 mg/mL papain (Worthington ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor tissues were mechanically and enzymatically dissociated using a human tumor dissociation kit (Miltenyi Biotec, Cat. No. 130-095-929), following the manufacturers recommendations ...
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Immunology 2020Quote: ... CD34+ HSCs were isolated from the cord blood and perfused placental tissue by using the CD34 Microbead Kit (Miltenyi Biotec) and stored at −150°C ...
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... lungs and spleens were collected in 5 ml MACS Tissue storage solution (Cat: 130-100-008; Miltenyi Biotec, Gaithersburg, MD) followed by storage at 4 °C until processing ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated into a single cell suspension using a neural tissue dissociation kit (Miltenyi Biotec; 130-092-628). Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea ...
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bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Grafts were flushed with saline and digested with collagenase A and DNase I in a gentleMACS tissue dissociator (Miltenyi Biotec). Cells were stained with cocktails of fluorochrome-conjugated monoclonal antibodies including AF647- conjugated anti-mouse CD57 (polyclonal ...
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bioRxiv - Genomics 2024Quote: Adrenal gland neoplasm samples were dissociated with 25-30 ul of enzyme D Multi Tissue Dissociation Kit (MTDK) (Miltenyi Biotec) or enzyme A Neural Tissue Dissociation Kit P (NTDK ...
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...