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Citations for Miltenyi Biotec :
201 - 250 of 555 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Immunology 2021Quote: ... anti-CD45RO and anti-CD14 magnetically tagged antibodies (MACS, Miltenyi Biotec, Auburn, CA) to deplete NK cells ...
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bioRxiv - Immunology 2021Quote: Human and mouse DCs were stained with the following antibodies from Miltenyi Biotec or as specified ...
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bioRxiv - Cancer Biology 2021Quote: ... and stimulated with particles coated with anti-CD3/anti-CD28 antibodies (Miltenyi Biotec) at a cell-to-bead ratio of 1:2 ...
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bioRxiv - Microbiology 2021Quote: ... purified mAbs were biotin-labelled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following the manufacturer’s recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 µL of Cd45-VioBlue antibody (REA737, #130-110-802, Miltenyi Biotec). After mixing well ...
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bioRxiv - Immunology 2021Quote: ... conjugated with a monoclonal anti-mouse B220 antibody (clone: RA3-6B2; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Two-fold dilutions of the anti-human CD303 BDCA2 antibody (AC144) (Miltenyi Biotec), chimeric CBS004 (Capella Bioscience ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used were: CD19 PE (LT19) and CD138 APC (B-B4; Miltenyi Biotec); CD23 APC (M-L233) ...
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bioRxiv - Neuroscience 2022Quote: ... Cell purity was assessed by anti-GLAST (ACSA-1) phycoerythrin antibody (Miltenyi Biotec) using flow cytometry (CyanADP Cytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... and a phycoerythrin (PE)-conjugated anti-CD133 antibody (Miltenyi Biotec, 130-098-826) followed by the fluorescence-activated cell sorting (FACS ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with FITC-conjugated anti-Ki-67 antibody (Miltenyi Biotec, Germany) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... purified mAbs were biotin-labeled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following manufacturer recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... an unconjugated mouse anti-human CD133 antibody was used (Miltenyi Biotec; 1:20), and for experiments including FAP ...
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bioRxiv - Immunology 2021Quote: ... 100 ng/ml of CD3-specific antibody (OKT-3; Miltenyi Biotec, Gladbach, Germany), and cultured in tissue culture flasks (TPP ...
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bioRxiv - Neuroscience 2021Quote: ... 2 µL of Cd11b-APC antibody (M1/70, #130-113-793, Miltenyi Biotec), and 2 µL of Cd45-VioBlue antibody (REA737 ...
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bioRxiv - Immunology 2021Quote: ... CD45- cells were enriched with magnetic-bead-conjugated anti-CD45 antibody (Miltenyi Biotec) before multicolor staining for flow cytometric cell sorting.
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were then labeled with CD14 antibodies conjugated to magnetic beads (Miltenyi Biotec). Magnetically labeled PBMCs were then passed through an LS column (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2023Quote: ... AtMCRS1-GFP was purified with anti-GFP antibody-conjugated magnetic beads (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... negative selection using anti-mouse biotinylated antibodies and anti-Biotin Microbeads (Miltenyi Biotec). Freshly isolated GC B cells were washed with PBS and allowed to attach/adhere onto Poly-L-Lysine coated coverslip (Corning ...
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bioRxiv - Immunology 2023Quote: ... injected with 3 μg of anti-mouse CD45-FITC antibodies (Miltenyi Biotec, Germany) retro-orbitally and left for 3 min prior to euthanasia by cardiac puncture ...
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bioRxiv - Immunology 2023Quote: ... CD45− cells were enriched in magnetic bead conjugated anti-CD45 antibody (Miltenyi Biotec). Multicolor flow cytometry and cell sorting were performed on FACSVerse ...
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bioRxiv - Immunology 2024Quote: ... and subjected to antibody-driven T-cell isolation using magnetic beads (Miltenyi Biotec). The isolated T cells were counted ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS, Miltenyi Biotec) in a 96-well round-bottom plate in complete RPMI medium for 5 days ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Late-stage Dd2 parasites in RBCs were magnetically purified from 5% sorbitol-synchronized cultures using MACS LD columns (Miltenyi Biotec) and seeded at 0.8-million RBCs/well in a Seahorse miniplate which was precoated with Cell-Tak cell and tissue adhesive (Corning) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Microbiology 2023Quote: ... lungs and spleens were collected in 5 ml MACS Tissue storage solution (Cat: 130-100-008; Miltenyi Biotec, Gaithersburg, MD) followed by storage at 4 °C until processing ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and homogenized in STKM buffer (250 mM Sucrose, 50 mM Tris-HCl, 25 mM KCl, 5 mM MgCl2) using the GentleMACS (Miltenyi Biotec) program Protein_01 ...
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Immunology 2022Quote: All cell culture was performed in a humidified incubator with 5% CO2 at 37°C using cRPMI (see above for ingredients) supplemented with 150ng/mL αCD40 (clone FGK-45; Miltenyi Biotec) and 25ng/mL recombinant murine IL-4 (Peprotech ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were stained with 5 μg/ml of propidium iodide in PBS and analyzed using a MACSquant analyzer (Miltenyi Biotec). Results were analyzed using Flowjo (https://www.flowjo.com).
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...