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Citations for Miltenyi Biotec :
51 - 100 of 364 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the Fc receptor was blocked (Miltenyi Biotec) and cells were stained with the following antibodies for 30 minutes at 4°C ...
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bioRxiv - Genetics 2022Quote: β-Thalassemia CD34+ HSPCs (Table S2) were enriched using the Miltenyi CD34 Microbead kit (Miltenyi Biotec). CD34+ HSPCs were thawed on day 0 into X-VIVO 15 (Lonza ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated with Fc blocking reagent (Miltenyi Biotec) for 10 minutes at room temperature before the addition of monoclonal antibodies for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in Fc receptor blocker (Miltenyi Biotec) diluted in PBS/2% BSA and incubated on ice for 10 min ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with Fc receptor blocker (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (v/v) containing FcR blocking reagent (Miltenyi Biotec) with fluorescently labeled antibodies as indicated in table 2 and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
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bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
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bioRxiv - Immunology 2021Quote: ... cultures were treated with Peptivator SARS-CoV2 Prot_S peptide pool (Miltenyi Biotec #130-126-700) in a 48-well plate format for 2 days.
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bioRxiv - Cancer Biology 2020Quote: ... and blocked using Fc Receptor block (Miltenyi Biotec 130-092-575). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... Nonspecific binding was blocked by using 1% Fc block (Miltenyi Biotec). Cells were stained with HLA-A2 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in human FC block (Miltenyi Biotec Inc., FLP3330) and kept on ice for 30 minutes ...
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bioRxiv - Plant Biology 2019Quote: ... immuno-purification was performed with µMACS GFP Tag Protein Isolation kits (Miltenyi Biotec). 1 mg of solubilized microsomes were loaded on µcolumns ...
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bioRxiv - Immunology 2021Quote: ... or overnight with 1 μg/peptide per well of spike associated (Cat: 130-127-951, Miltenyi Biotec Peptivator) peptides at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The splenocytes were incubated with the spike protein peptide pool at 1.5 μg/ml/peptide (Miltenyi Biotec, Germany) at 37 °C for 16-18 hours ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4C with anti-human Fc Block (Miltenyi Biotec). Cells were incubated with DAPI viability dye (Thermo ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated in ice with Fc block reagent (Miltenyi Biotec) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and blocked with PBS supplemented with 1% Fc Block (MACS, Miltenyi Biotec) and 0.5% BSA for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... and Fc-receptors were blocked by the mouse FcR Blocking Reagent (Miltenyi Biotec)(0.5μL/well ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated on ice with an Fc block reagent (Miltenyi Biotec) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc receptors were blocked using Fcr Blocking Reagent (Miltenyi Biotec, 130-059-901) according to manufacter’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Cells were blocked with mouse Fc receptor block (Miltenyi Biotec, San Diego, CA) and stained with conjugated primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Fc block was performed using FcR blocking reagent (Miltenyi Biotec, 130-092-575). Staining buffer consisted of PBS with 2 mM EDTA and 0.5% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
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bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
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bioRxiv - Immunology 2020Quote: ... with 20% FCS or a whole skin digestion kit from Miltenyi Biotec (Cambridge, U.K.). Single cell suspensions were obtained and subsequently stained with the following antibodies against ...
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bioRxiv - Microbiology 2023Quote: ... Recovered cells were incubated with Fc block (Miltenyi Biotec, Bergusch Gladbach, Germany, 1:100), vortexed ...
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bioRxiv - Cell Biology 2022Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cell Biology 2021Quote: ... either MNCs or CD34 enriched cells were thawed and washed and stained with FC block (Miltenyi Biotec) for 10 min and washed again ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Microbiology 2021Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to electroporation.
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bioRxiv - Microbiology 2022Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to infection.
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...