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Citations for Miltenyi Biotec :
51 - 100 of 158 citations for Recombinant Rat Il4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Sorted cells were treated with 50 ng/mL recombinant stem cell factor (SCF; Miltenyi Biotec), 5 μM imatinib mesylate (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
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bioRxiv - Cell Biology 2024Quote: ... The enzyme mix from Neonatal Heart Dissociation Kit mouse and rat (Miltenyi Biotec) was added to the tube and placed into the gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Immunology 2023Quote: ... before dissociating using an Adipose Tissue Dissociation Kit for Mouse and Rat (Miltenyi Biotec), using a gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Immunology 2023Quote: iMSC-like cells were acquired by stimulating ADSCs with recombinant human IL-1β (0.5ng/ml, Miltenyi Biotec) overnight ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2021Quote: ... Human recombinant interleukin-7 (IL-7) and interleukin-15 (IL-15) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Retronectin (Recombinant Human Fibronectin ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were subsequently stained with IL-17-FITC (1:20, rat, Miltenyi Biotec, 130-102-262), CD-3 APC/cy7 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: Primary astrocytes were isolated from P0/P1 rat pup cerebral cortices using vendor established protocols (Miltenyi Biotec) and seeded on coated plates (30,000 cells/well in 96 well or 1 million cells/well in 6-well) ...
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bioRxiv - Neuroscience 2023Quote: ... Neural cells were incubated with CD11b+ magnetic microbeads (Miltenyi Biotec, CD11b/c Microbeads, rat, 130-105-634) and CD11b+ cells positively selected through a magnetic column to enrich for microglial cells ...
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation to isolate the protein complexes from total protein extracts using GFP-tagged microbeads (Miltenyi Biotec) has been described previously [8] ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Cell Biology 2021Quote: ... Interacting proteins were isolated by applying total protein extracts to α-GFP-coupled magnetic beads (Miltenyi Biotec). Three replicates of TPLATE motif substitution mutants (SWM and PFM ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:10 50nm-magnetic-bead antibody solution against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523). Nerve fibroblasts express the marker CD90.1 ...
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bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μl of 50nm-magnetic-bead antibodies against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523) and incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
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bioRxiv - Systems Biology 2019Quote: Cells were washed once with PBS and stained with CD11b-APC or isotype control (Rat IgG2b-APC, Miltenyi Biotec) antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... Interacting proteins were isolated from total protein extracts using anti-GFP-coupled magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Proteins were digested with trypsin to peptides ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
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bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Developmental Biology 2020Quote: ... The separation of STO cells from rat spermatogonial stem cells was carried using the Magnetic activated cell sorting (MACS) from Miltenyi Biotec.
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Immunology 2020Quote: ... and spike proteins (Prot_S) were purchased from Miltenyi Biotec. Optimal peptides for the manufacture of HLA class I tetramers were synthesized at >95% purity by Peptides & Elephants GmbH ...
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bioRxiv - Cell Biology 2019Quote: The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Cell Biology 2021Quote: Different lineages of in vitro cultured endMSCs (n = 3) were primed using a combination of recombinant pro-inflammatory cytokines and Toll-like receptors (TLR) ligands: IFNγ (Miltenyi Biotec Inc, San Diego, CA, USA) at 1 ng/ml and 100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were collected from wistar rat at P5 and cortices and corpus callosum were dissected and dissociated using neural tissue dissociation kit (P) (Miltenyi Biotec, Paris, France) with gentle MACS Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were eluted with 100 μL elution buffer (Miltenyi Biotec). Proteins were resolved in 8% SDS-PAGE gels described above.
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bioRxiv - Immunology 2020Quote: Protein A beads and µ columns were from Miltenyi Biotec Ltd. ...
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bioRxiv - Neuroscience 2021Quote: ... and the brains were extracted and dissociated using Miltenyi Adult mouse and rat Brain Dissociation Kit and the gentleMACS Octo Dissociator (Miltenyi Biotec 130-107-677). The cells were then resuspended in PBS + 0.5% BSA and stained with DAPI to determine viability ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... Co-immunoprecipitated proteins were isolated using magnetic μMACS columns (Miltenyi Biotec) as previously described61 ...
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bioRxiv - Microbiology 2022Quote: ... then 50 μL of protein G conjugated nanobeads (Miltenyi Biotec, Germany) were added on samples ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were eluted off the beads with elution buffer (Miltenyi Biotec). Proteins were resolved using SDS polyacrylamide gel electrophoresis and gels were sent to the Rutgers Biological Mass Spectrometry Facility ...
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bioRxiv - Plant Biology 2023Quote: ... Protein-DNA complexes were immunoprecipitated using anti-HA antibodies (Miltenyi Biotec) (Kaufmann et al. ...
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bioRxiv - Physiology 2023Quote: ... Hearts were cut in small pieces and dissociated at 37°C with the commercially available enzyme mix (Neonatal Heart Dissociation Kit, mouse and rat, Miltenyi Biotec GmbH; # 130-098-373) followed by tissue homogenization with the genteMACS™ Dissociator (Miltenyi Biotec GmbH ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were detected with α-GFP-HRP (Miltenyi Biotec, 130-091-833), α-Myc-HRP (Abcam ...