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Citations for Miltenyi Biotec :
51 - 100 of 1152 citations for Recombinant Mouse Scavenger Receptor Class B Member 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Primary murine peritoneal cells were sorted into B-1a and B-1b cells using MACS B-1a selection kit (Miltenyi Biotec inc.). Sorted cells were cultured in RPMI1640 containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Immunology 2021Quote: ... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
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bioRxiv - Immunology 2020Quote: ... or recombinant IFN-α (Miltenyi Biotec), recombinant IL-12 (R&D systems and Peprotech ...
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bioRxiv - Immunology 2020Quote: ... or recombinant IL-15 (Miltenyi Biotec) as described in the text and figure legends ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Flt3-ligand (Miltenyi Biotec), recombinant human IL-6 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human IL-6 (Miltenyi Biotec), recombinant human TPO (PeproTech) ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched using a B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) from spleens of immunized mice ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were negatively sorted using the Pan B cell isolation kit (Miltenyi Biotec). Induction of germinal center B-cell differentiation was done following the protocol of T ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Biochemistry 2021Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... APC conjugated anti-HIS antibody (Miltenyi Biotec, Auburn, CA) was used as secondary antibody at 1:50 dilution ...
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bioRxiv - Immunology 2023Quote: ... then stained with fluorescent anti-His antibody (Miltenyi Biotec), along with other antibodies according to panels described in Supplementary Table 6 ...
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bioRxiv - Plant Biology 2023Quote: ... µMACS magnetic beads conjugated to anti-His (Miltenyi Biotec) were added and incubated for 1 h at 4°C to retrieve the bait protein together with interacting proteins ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were isolated from PBMCs by using CD19+ B cell isolation kits (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... and total splenic B cells were purified using a negative B cell isolation kit (Miltenyi Biotec). Purity of enriched B cells was determined by flow cytometry ...
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bioRxiv - Immunology 2024Quote: B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc receptors were blocked using Fcr Blocking Reagent (Miltenyi Biotec, 130-059-901) according to manufacter’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant BMP4 was obtained from Miltenyi Biotec and SB431542 was obtained from Tocris Bioscience ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-Glast (Miltenyi Biotec, 1:100), rat anti-CD133 (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-GLAST (1: 300, Miltenyi Biotec), mouse anti-caveolin1 (1:50 ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Immunology 2019Quote: ... Total B-cells were isolated by negative selection with the Memory B-cell Isolation Kit (Miltenyi Biotec). Memory-enriched B cells fractions were isolated by negative selection following incubation of total ...
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bioRxiv - Immunology 2021Quote: ... total PMBCs were enriched for B cells using a MACS human B cell isolation kit (Miltenyi Biotec). Purified B cells were enumerated ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were then isolated with Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... anti-His-PE (mIgG1) (Miltenyi Biotec Swiss AG, Solothurn, Switzerland), and LIVE/DEAD™ Fixable Near-IR stain (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-His-HRP (Miltenyi Biotec, Cat# 130-092-785).
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bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Microbiology 2022Quote: ... recombinant anti-DC-SIGN (clone REA617, Miltenyi Biotec), mouse anti-CD1a (Novus Biologicals) ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... B cells were isolated by negative selection using the B Cell Isolation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). The procedure was carried out following the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... B cells and T cells (Miltenyi Biotec) were used ...
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bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Immunology 2021Quote: ... anti-CD45RO and anti-CD14 magnetically tagged antibodies (MACS, Miltenyi Biotec, Auburn, CA) to deplete NK cells ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec), recombinant human Flt3-ligand (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Memory B cells and naïve B cells were isolated from RosetteSep pre-sorted total B cells using MACS cell sorting from Miltenyi Biotec. Memory B cells were obtained via positive selection using the Milteny Memory B cell isolation kit ...
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bioRxiv - Molecular Biology 2023Quote: ... CD43− B cells were enriched from the spleen by immunomagnetic depletion of non-B cells with CD43-MicroBeads (Miltenyi Biotec). The following monoclonal antibodies were used for flow-cytometric analysis of mouse lymphoid organs from 4-12 week-old mice ...