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Citations for Miltenyi Biotec :
1 - 50 of 837 citations for Recombinant Mouse Cd4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Immunology 2021Quote: ... or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit, Miltenyi Biotec, Auburn, CA, USA), using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were enriched from the spleen cells by using mouse CD4 MACS Beads (L3T4, Miltenyi Biotec) and then CD4+CD44loCD62LhighFoxp3-eGFP- naïve T cells were purified by sorting with flow cytometry (FACS Aria III ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were isolated by MACS using the mouse CD4+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS/FCS and naïve CD4+ T cells were isolated using the naïve CD4+ T cell isolation kit (mouse) from Miltenyi Biotec according to their standard protocol ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2023Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Microbiology 2020Quote: ... 5 million units per kg recombinant mouse IFNα (Miltenyi Biotec) were injected intravenously ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Immunology 2021Quote: ... tuberculosis Erdman using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Cells were isolated using a mouse CD4+ T cell isolation kit (Miltenyi Biotec), and stimulated with 5 μg/ml plate-bound anti-CD3ε (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Spleens from four- to six-week-old mice were processed to single cell suspensions and CD4+ T cells were enriched using mouse CD4 (L3T4) Microbeads kit (Miltenyi Biotec Cat # 130-117-043) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... mouse splenocytes were subjected to ACK lysis followed by anti-CD4 positive selection (Miltenyi Biotec). Naive CD4+ T cells (CD4+CD25-CD44-CD62L+ ...
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were enriched via magnetic separation using CD4 MicroBeads (Miltenyi Biotec) and surface stained as described above ...
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bioRxiv - Immunology 2019Quote: ... Purified CD4+ T-cells were stained with CD4 antibody (Biotin, Miltenyi Biotec) and CD25 antibody (PE ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were purified using anti-CD4+ conjugated microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and CD4+ T PbTII cells were enriched using CD4 microbeads (Miltenyi Biotec). Cells (104/200 µl ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched using CD4 magnetic beads (CD4 L3T4 Kit, Miltenyi Biotec) and 1X106 CD4+ cells transferred i.p ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse CD4+ T cells were cultured in complete TexMACS Medium (Miltenyi Biotec, #130-097-196) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were isolated using positive selection with mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... CD4-PE (Miltenyi Biotec) to detect cytotoxic and helper T cells respectively and B220 biotin RA3-6B2 Alexa Fluor 647 to detect B cells (CD45R) ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched using CD4 microbeads (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2019Quote: ... with CD4 T cells depleted using CD4+ magnetic beads (Miltenyi Biotec, Auburn, CA), and then irradiated with 3000 rads ...
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bioRxiv - Immunology 2023Quote: ... and the CD4+ PbTII cells were enriched using CD4 (L3T4) microbeads (Miltenyi Biotec). Each mouse received 104 PbTII cells or 15×106 OB1 cells injected into the lateral tail veins.
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bioRxiv - Immunology 2023Quote: ... and CD4+ PbTII GFP+ cells were enriched using CD4 (L3T4) microbeads (Miltenyi Biotec). To perform CRISPR/Cas9 knockout in naïve PbTII GFP+ cells ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ T cells were enriched using MACS CD4 (L3T4) microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ T cells were further isolated using CD4+ T cell isolation kit (Miltenyi Biotec). The isolated cells were cultured in RPMI medium supplemented with 20% (v/v ...
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bioRxiv - Immunology 2021Quote: ... and naive CD4+ T cell using the Naive CD4+ T Cell Isolation (Miltenyi Biotec). T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2022Quote: ... we positively selected CD4+ T cells using CD4 MicroBeads and magnetic sorting (Miltenyi Biotec). Enriched CD4+ T cells were counted ...
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bioRxiv - Cancer Biology 2023Quote: ... enriched for CD8+ and CD4+ T cells using CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), and labeled with CFSE (5 M ...
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bioRxiv - Immunology 2021Quote: ... CD4-PercP (VIT4, Miltenyi Biotec), CD25-APC (2A3 ...
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bioRxiv - Immunology 2021Quote: ... CD4-PE (REA623, Miltenyi Biotec), CCR6-PerCP (G034E3 ...
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bioRxiv - Immunology 2021Quote: ... CD4-PerCP (VIT4, Miltenyi Biotec), CXCR5-BV421 (J252D4 ...
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bioRxiv - Immunology 2021Quote: ... CD4-PercP (VIT4, Miltenyi Biotec), CXCR3-APC (1C6 ...
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bioRxiv - Microbiology 2022Quote: CD4+ T cells were isolated from PBMC population using the CD4+ isolation kit (CD4+ T cell Isolation kit II, Miltenyi Biotec, San Diego, CA). CD4+ T cells were first cultured for three days using stimulating conditions (RPMI supplemented with 10% donor autologous serum ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were then separated from the white cells using mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... CD4+CD25- T cells were isolated using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and CD25 Microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25− Th cells were purified by positive selection with CD4-specific microbeads (Miltenyi Biotec). Memory T-cell subsets were sorted to more than 97% purity as CD4+CD45RA− RO+CD127+CD25− using APC-780-conjugated anti-CD45RA Ab (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were positively selected with CD4 (L3T4) magnetic microbeads (Miltenyi Biotec, Auburn, CA), pooled and suspended in PBS ...
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bioRxiv - Microbiology 2022Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...