Labshake search
Citations for Miltenyi Biotec :
351 - 400 of 662 citations for Recombinant Human Epidermal Growth Factor Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CD11b MicroBeads (mouse/human; Miltenyi Biotec Inc., Auburn, CA) and passed through MACS® LS magnetic columns for positive selection of CD11b+ cells ...
-
bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
-
bioRxiv - Immunology 2019Quote: ... filtered human AB serum then subjected to gentle mechanical dissociation using gentleMACS (Miltenyi Biotec) followed by short 20 min enzymatic dissociation at 37°C using a very low concentration of Liberase TL (0.042 mg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... PBMCs were incubated with anti-human CD14 antibodies coupled to magnetic beads (Miltenyi Biotec) and subjected to magnetic separation using LS columns (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: Human and mouse lung tissues were digested using the Tumor Dissociation Kit (Miltenyi Biotec) for 30 minutes at 37°C and 1,500 rpm ...
-
bioRxiv - Immunology 2020Quote: ... total PBMC were enriched in CD14+ monocytes using the human CD14 microbeads (Miltenyi Biotec) for positive magnetic selection according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... T cells were isolated using the human Pan T cell Isolation Kit (Miltenyi Biotec) and naive CD4+ T cell using the Naive CD4+ T Cell Isolation (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
-
bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... PE CD158a/h (KIR2DL1/DS1) anti-Human antibody: 130-099-209 from Miltenyi Biotec. APC CD158b1/b2j (KIR2DL2/DL3/DS2 ...
-
bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec, 130-120-709) for 1 h at room temperature in the dark in 0.75% saponin (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... human cells were incubated with anti-biotin microbeads (Miltenyi Biotec, Cat#130-090-485) in MACS buffer at 4°C for 15 min ...
-
bioRxiv - Immunology 2022Quote: ... All setups were stained with 5μL of human FcR blocking reagent (Miltenyi Biotec, USA) to mitigate unspecific binding and LIVE/DEAD blue stain (Invitrogen ...
-
bioRxiv - Genetics 2022Quote: ... Human bone marrow CD45+ cells were sorted by immunomagnetic selection with AutoMACS (Miltenyi Biotec) after immunostaining with the CD45 MicroBead Kit (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2023Quote: ... and CD38 (20 µl per 107 cells, from human CD38 MicroBead kit, Miltenyi Biotec) followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells ...
-
bioRxiv - Microbiology 2022Quote: ... CD8 T cells were depleted using a kit from Miltenyi Biotec (CD8 MicroBeads, human) and cells cultured in RPMI-1640 (Life Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Enzymes and reagents from the human tumor dissociation kit (Miltenyi Biotec: 130-095-929) were added to the tumor tissue along with 1.75 mL of DMEM ...
-
bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
-
bioRxiv - Immunology 2024Quote: ... Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec). Monocytes were then plated 0.15×106/well onto 96-well plate (for ELISA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Thawed and fresh samples were dissociated using the Tumor Dissociation Kit (human, Miltenyi Biotec) and tough dissociation protocol on gentleMACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Genomics 2020Quote: ... Structures were enzymatically dissociated into single cells using the Tumor Dissociation Kit (human) (Miltenyi Biotec) with a gentleMACS™ Octo Dissociator (with heaters ...
-
bioRxiv - Immunology 2019Quote: ... CD4 T cells were isolated from healthy human donor PBMCs by negative selection (Miltenyi Biotec), and activated with plate-bound anti-CD3 (clone OKT3 ...
-
bioRxiv - Cell Biology 2021Quote: Human neutrophils were isolated on a MACS LS column system (Miltenyi Biotec #130-042-401), enriched with magnetic anti-CD15 antibodies (Miltenyi Biotec #130-046-601 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and anti-human CD38 (IB6, PE, Miltenyi Biotec, Cat No 130-113-989, 1:50) antibodies ...
-
bioRxiv - Immunology 2022Quote: ... resting NK cells were obtained using the human NK cell isolation kit (MACS Miltenyi Biotec), following manufacture’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
-
bioRxiv - Bioengineering 2022Quote: ... Human monocytes were isolated from PBMC using a magnetic-based monocyte isolation kit (Miltenyi Biotec) and differentiated into macrophages with GMCSF (50 ng/mL ...
-
bioRxiv - Systems Biology 2020Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (MACS Miltenyi Biotec) and LS Columns (Miltenybiotec) ...
-
bioRxiv - Immunology 2019Quote: ... human CAR T cells were sorted for CD34+ (CD34 MicroBead Kit UltraPure, MACS, Miltenyi Biotec) CAR T cells and expanded for up to 15 days in the presence of human IL-2 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... T cells were stimulated and expanded using TransAct Human T cell activation reagent (Miltenyi Biotec). For transduction ...
-
bioRxiv - Immunology 2019Quote: ... density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201, Miltenyi Biotec). Monocytes were incubated for 7 days with RPMI-1640 (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... HDMECs were then isolated using a human CD31 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... monocytes were purified from human PBMC by using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
-
bioRxiv - Genomics 2020Quote: ... anti-human EPCAM antibody conjugated to PE (1:50 dilution, Miltenyi Biotec #130-091-253), and either Calcein-AM (1:200 dilution ...
-
bioRxiv - Immunology 2022Quote: ... CD8 or CD4 T cells were purified from human PBMCs using isolation kits (Miltenyi Biotec) following manufacturer’s instruction ...
-
bioRxiv - Immunology 2022Quote: ... T cells were purified using the human Naïve Pan T Cell Isolation Kit (Miltenyi Biotec) and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were treated with FcR-blocking reagent and human CD34 Microbeads (Miltenyi Biotec, Gaithersburg, MD) for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... and digested using the Tumor Dissociation Kit for human tissue (Miltenyi Biotec: #130-095-929) on a gentle MACS Octo Dissociator with heaters (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2023Quote: ... CD14+ monocytes were then obtained by positive magnetic isolation using human CD14 microbeads (Miltenyi Biotec) and a MACS LS column with a QuadroMacs™ separator (Miltenyi Biotech) ...
-
bioRxiv - Cancer Biology 2023Quote: ... human according to manufacturer instructions and the GentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) using custom settings ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT ...
-
bioRxiv - Cancer Biology 2024Quote: ... The freshly isolated PBMCs were subsequently cultured in human NK MACS® medium (Miltenyi Biotec) for 2 to 3 weeks to ensure optimal in vitro expansion of NK cells ...
-
bioRxiv - Biochemistry 2019Quote: ... and subsequent CD14+ positive selection using the MACS Miltenyi Biotec Human CD14 microbead protocol (Miltenyi Biotec). CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF ...
-
bioRxiv - Cell Biology 2020Quote: ... or 20 ng/ml PDGF-AA (human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec). Inhibition of PDGF-signaling was performed by addition of 1μM imatinib (Cat nb ...
-
bioRxiv - Neuroscience 2022Quote: ... the cells were labeled with human CD11b-APCVio770 and CD45-phycoerythrin (PE) (Miltenyi Biotec, Gladbach, Germany), and flow cytometry was performed as described previously (38) ...
-
bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
-
bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using the Human Pan T cell isolation kit (Miltenyi Biotec) and subsequently activated by TransAct (Miltenyi Biotec) ...