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Citations for Miltenyi Biotec :
101 - 150 of 668 citations for Recombinant Human CD19 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Freshly isolated PBMCs and CD19-depleted PBMCs were cultured in TexMACS™ medium (Miltenyi Biotec, Bergisch Gladbach, Germany), an optimised T-cell medium ...
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bioRxiv - Immunology 2021Quote: ... anti-CD45RO and anti-CD14 magnetically tagged antibodies (MACS, Miltenyi Biotec, Auburn, CA) to deplete NK cells ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Immunology 2019Quote: ... Either PBMC or B cells magnetically sorted using the AutoMACS Pro cell separator and CD19+ microbeads (both Miltenyi Biotec), were lysed in RLT buffer (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... Spleen qPCR samples were prepared by depletion of CD19+ TCRβ+ cells using biotinylated antibodies and Streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human (Miltenyi Biotec), per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... human (Miltenyi Biotec) protocol ...
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bioRxiv - Cell Biology 2020Quote: ... human (Miltenyi Biotec), according to the manufacturer’s instructions [44].
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bioRxiv - Cancer Biology 2020Quote: ... human (Miltenyi Biotec) at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... human (Miltenyi Biotec) was used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec), and cultured in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Human (Miltenyi Biotec) and human CD45+ cells sorted by flow cytometry on a FACS Aria II instrument.
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec) through a magnetic column.
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec). The purity evaluated by flow cytometry was routinely >90%.
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec). The dCODE Dextramer reagents (HiT ...
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bioRxiv - Molecular Biology 2022Quote: ... human (Miltenyi Biotec) via CD3 and CD28 for 24hrs.
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bioRxiv - Neuroscience 2023Quote: ... human (Miltenyi Biotec.). Purity was confirmed to be over 90 % by flow cytometry ...
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bioRxiv - Molecular Biology 2023Quote: ... Human (Miltenyi Biotec), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Human (Miltenyi Biotec) for 60 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... human (Miltenyi Biotec) for 10 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Human (Miltenyi Biotec) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... 5 million units per kg recombinant mouse IFNα (Miltenyi Biotec) were injected intravenously ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were depleted of B and NK cells via CD19+ and CD56+ microbeads according to the manufacturer’s protocol (Miltenyi Biotec). M1- and M2- macrophages were generated from blood monocytes in vitro ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Immunology 2023Quote: Splenic CD4+ T cells derived from OT-II TCR-Tg mice were enriched with Biotin-labeled antibody cocktail (B220, CD8a, CD11b, CD11c, CD19, CD25, NK1.1, and TER-119) with Streptavidin Microbeads (Miltenyi Biotec). CD4+ T cells were further incubated with CD62L Microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... The purity of isolated B cells was over 95% as assessed by flow cytometry analysis of PE-CD19+ cells (Miltenyi Biotec) 87,88.
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bioRxiv - Immunology 2022Quote: Aged splenocytes were depleted of RBC and CD19+B220+ cells were enriched by positive selection of CD43 and Ter-119 cells and LD MACS columns (Miltenyi Biotec). CD43-Ter-119- splenocytes were stained with FITC anti-B220 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... To determine proliferation and IFNγ production upon in vitro TCR-mediated stimulation splenocytes were enriched for T cells using CD19 microbeads depletion (Miltenyi Biotec) on LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Immunology 2023Quote: A cluster of differentiation 19 (CD19)-positive B lymphocytes (total B cells) were purified from buffy coats derived from healthy donors using magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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bioRxiv - Bioengineering 2023Quote: ... Human TNF-α and human IFN-γ were purchased from Miltenyi Biotec. Human TNF-α DuoSet ELISA ...
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bioRxiv - Molecular Biology 2020Quote: ... human (Miltenyi Biotec Inc). See table S4 for antibodies used in flow cytometry.
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bioRxiv - Cell Biology 2022Quote: ... human (Miltenyi Biotec, USA) following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2022Quote: ... human (Miltenyi Biotec, USA). Purified iPSC-cardiomyocytes were then plated on either Matrigel-coated glass coverslips (immature cells ...
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bioRxiv - Physiology 2022Quote: ... human (Miltenyi Biotec, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... human (Miltenyi Biotec, Germany) following protocols provided by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... and then with antibody (1:5000 anti-His-HRP (130-092-785, Miltenyi Biotec) or anti-FLAG-HRP (Ab49763 ...
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation to isolate the protein complexes from total protein extracts using GFP-tagged microbeads (Miltenyi Biotec) has been described previously [8] ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: RNA immunoprecipitation (RIP) assays were performed with the μMACS GFP-Tagged Protein Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Immunology 2023Quote: ... and following COVID-19 infection underwent CD19 and CD3 enrichment utilizing MicroBeads and LS Columns on the QuadroMACS™ Separator (Miltenyi Biotec). Single-cell suspensions of ∼1,200 cells/µl in PBS were prepared for scRNA-seq using 10X Chromium Next GEM Single Cell 5’ Kit v2 also using the Chromium Single Cell Human TCR and BCR Amplification Kits (10x Genomics ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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Stability and heterogeneity in the anti-microbiota reactivity of human milk-derived Immunoglobulin AbioRxiv - Immunology 2023Quote: ... APC Anti-Human IgA [Anti-Human IgA APC (Miltenyi Biotec clone REA1014, (1:50)] ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...