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Citations for Miltenyi Biotec :
51 - 100 of 794 citations for Recombinant Human CD14 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec), recombinant human Flt3-ligand (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
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bioRxiv - Immunology 2020Quote: ... Immature human moDCs were generated from PBMCs using CD14 Microbeads (Miltenyi Biotec cat. 130-050-201) and cultured for 6 days with recombinant GM-CSF (R&D Systems cat ...
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bioRxiv - Biochemistry 2019Quote: CD14+ cells in bulk PBMCs were labeled using CD14 MicroBeads (Miltenyi Biotec) and purified using a MACS LS Column on a MidiMACS Separator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2019Quote: ... CD14+ monocytes were isolated by positive immunoselection (CD14 MicroBeads, Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ monocytes were isolated by positive selection using CD14 microbeads (Miltenyi Biotec) and were cultured at 37°C and 5% CO2 in RPMI-1640 medium (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... CD14+ microbeads (Miltenyi Biotec) were used to select monocytes according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... CD14 microbeads (Miltenyi Biotec) were added to the cell suspension at 20 μL per 107 cells and incubated for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... CD14+ microbeads (Miltenyi Biotec) were used to select monocytes according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human transforming growth factor β1 (premium grade, Miltenyi Biotec) was diluted and used at the indicated concentrations.
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant TGF-β1 50 ng/mL (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD14+ cells were positively selected using anti-CD14-conjugated magnetic microbeads (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: CD14+ cells were purified from frozen PBMCs and CD14 positive selection (Miltenyi Biotec) according to manufacturers’ protocols ...
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bioRxiv - Immunology 2024Quote: ... we cultured autologous CD14+ monocytes isolated by positive selection (CD14 MicroBeads; Miltenyi Biotec) in X-Vivo15 medium (BioWhittaker/Lonza) ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE-Vio® 770 anti-human CD14 (clone TÜK4, dilution 1/50, Miltenyi Biotec, Paris, France) antibodies were used for macrophages phenotyping ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in human FC block (Miltenyi Biotec Inc., FLP3330) and kept on ice for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... CD14+ cells were enriched from PBMCs via magnetic separation using CD14 MicroBeads (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: Monocytes were isolated using a CD14+ magnetic positive selection kit (CD14 Microbeads, Miltenyi Biotec) from patients PBMCs ...
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bioRxiv - Immunology 2020Quote: CD14+ monocytes (purity >98%) were depleted through positive selection using CD14 MicroBeads (Miltenyi Biotec). CD4+ T cells were isolated from the CD14− cell fraction by negative selection (Miltenyi Biotec ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ PBMCs were enriched using anti-CD14 antibody microbeads (Miltenyi Biotec #130-050-201), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD14+ monocytes were isolated from PBMC utilizing CD14 microbeads (Miltenyi Biotec, Auburn, CA, USA) as previously described [15] ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Pathology 2021Quote: ... Human recombinant OSM was added at 100 ng/ml (Miltenyi Biotec). Cells were cultured for 14 days at 37°C in 5% CO2 atmosphere ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) ...
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bioRxiv - Immunology 2024Quote: ... with 30 U/ml of recombinant human IL-2 (Miltenyi Biotec). T cells were cultured in AIMV (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) and growth factor-reduced Matrigel (Corning ...
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bioRxiv - Immunology 2022Quote: ... CD14 (clone TUK4, Miltenyi Biotec), CD11b (clone ICRF44 ...
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bioRxiv - Immunology 2021Quote: ... CD14-VioGreen (TUK4, Miltenyi Biotec), CD8-VioGreen (BW135/80 ...
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bioRxiv - Immunology 2020Quote: ... anti-CD14 (TUK4, Miltenyi Biotec), anti-CD16 (3G8 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CD14 (REA599, APC, Miltenyi Biotec), CD4 (VIT-4 ...
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bioRxiv - Cancer Biology 2024Quote: ... CD14-PerCP (TUK4, Miltenyi Biotec) CD86-PE-Cy7 (2331 ...
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bioRxiv - Pathology 2024Quote: ... and CD14 MicroBeads (Miltenyi Biotec) were added to the isolated peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2023Quote: ... according to the manufacturer’s suggestions and monocytes purified using positive selection with paramagnetic CD14 (human) MicroBeads (Miltenyi Biotec). CD14+ monocytes were differentiated into macrophages using 100 ng/mL of recombinant human M-CSF (Immunotools ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4C with anti-human Fc Block (Miltenyi Biotec). Cells were incubated with DAPI viability dye (Thermo ...
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bioRxiv - Cell Biology 2022Quote: ... and monocyte populations (>97% CD14+) were isolated with CD14-positive selection magnetic beads (Miltenyi Biotec). DCs were obtained by culturing these cells in the presence of 1,000 IU/ml granulocyte-macrophage colony-stimulating factor and interleukin-4 (both from R&D ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... CD14+ monocytes were isolated by positive selection using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and were allowed to differentiate into Mφs in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were isolated by magnetic activated cell sorting (CD14+ Cell Isolation Kit; Miltenyi Biotec). 2×10⁵ monocytes were then differentiated into M1-type macrophages cells by incubation with 1000 U/ml GM-CSF (granulocyte macrophage-colony stimulating factor ...
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bioRxiv - Immunology 2021Quote: ... CD14 cells were purified by anti-CD14 monoclonal antibody (mAb)-conjugated magnetic microbeads (Miltenyi Biotec) and then cultured for 6 days in RPMI 1640 medium (Life Technologies Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ cells were isolated from PBMNCs using a magnetic micro-bead CD14+ kit (Miltenyi Biotec), LS columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and monocytes were magnetically sorted with magnetic microbeads coupled with antibodies directed against CD14 (Miltenyi Biotec #130-050-201). Monocytes were then seeded on glass coverslips at 1.5×106 cells/well in six-well plates in RPMI 1640 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5ng/ml recombinant human GM-CSF (Miltenyi Biotec, 130-093-866). HEK293T cells (ATCC CRL-3216 ...
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bioRxiv - Immunology 2022Quote: ... and 100IU/ml recombinant human IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany) were added ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (Miltenyi Biotec; Cat. No. 130-050-201) and LS Columns (Miltenyi Biotec ...