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Citations for Miltenyi Biotec :
151 - 200 of 3329 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were harvested from femur and tibiae of 6–9 week-old CD11c-DTR or CD11c-DTR Rag2−/− SJL mice and depleted of T cells using anti-CD3 and anti-CD8 magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). Wild type or Rag2−/− SJL mice were gamma-irradiated with 950 rads from a 60Co source ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were depleted for CD4 and CD8 T cells using magnetic bead depletion techniques according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... cells were prepared from CD45.1 Thy1.2 C57Bl/6 mice by first crushing bones followed by T cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). CD45.2 Thy1.2 FoxP3/GFP+ CD4+ Treg were FACS sorted from CD8/CD19-depleted single cell suspensions from spleens and lymph nodes from CD45.2 Thy1.2 FoxP3GFP+ C57Bl/6 using a BD FACS Aria II ...
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bioRxiv - Immunology 2021Quote: ... and purified their T cells using the untouched isolation kit (Pan T Cell Isolation Kit II, mouse, 130-095-130, Miltenyi Biotec).
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bioRxiv - Biophysics 2019Quote: ... T cells were isolated from PBMCs with Pan T cell isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), then were stimulated for 48⍰h with anti-CD3/anti-CD28 Dynabeads (Gibco by Thermo Fischer Scientific ...
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bioRxiv - Immunology 2019Quote: ... CTV-labeled T cells were then isolated using a Pan naïve T cell isolation kit (Miltenyi Biotec) according to manufacturer’s instructions and isolation purity (≥95% ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Splenic T cells were purified using T cell isolation kits and AutoMacs magnetic separation system (Miltenyi Biotec) and approximately 90% of the purified cells displayed a naïve phenotype as previously described19 ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD4 T cells were isolated using the Naïve CD4 T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
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bioRxiv - Immunology 2020Quote: ... γδ T cells were isolated from this suspension using the γδTCR+ T cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were then enriched using a nonhuman primate CD4+ T cell isolation kit (Miltenyi Biotec). 1×104 ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were purified from spleens with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec), activated and cultured as previously described (11).
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bioRxiv - Immunology 2024Quote: ... untouched Pan T cells were enriched by negative selection using T cell isolation kit II (Miltenyi biotec). All donor tissue information is included in Table S1.
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the spleenocytes using CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were isolated from the spleens of uninfected or MHV-A59 infected animals using the untouched CD8+ T cell isolation MACS kit from Miltenyi Biotec (cat#130-104-075, Gaithersburg, MD) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... CD8 cells were depleted from the PBMCs using CD8 microbeads (Miltenyi Biotec, Germany) according to the Manufacturer’s instructions and 1.5 × 106 cells were infected using 1 mL virus supernatant by spinoculation (1125 × g ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were labeled with a rat anti-CPM antibody (in-house) and microbead-conjugated anti-rat immunoglobulin kappa light chain antibody (130-047-401, Miltenyi Biotec). This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Spleens of OT-I Hobit reporter × ROSA26-eYFP LT mice were isolated and subsequently CD8+ T cells were isolated by negative selection using MicroBeads (130-104-075, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Immunology 2023Quote: SB CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Immunology 2023Quote: LV CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell suspensions of CD4+ or CD8+ T-cells were prepared from spleens by negative selection using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Naive 6-week-old IFN-αβR-/- mice (n = 4-9 ...
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bioRxiv - Immunology 2021Quote: ... or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit, Miltenyi Biotec, Auburn, CA, USA), using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... CD4 T cells were purified by negative selection using the human CD4 T Cell Isolation Kit (Miltenyi Biotec). T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was removed and T-cells were isolated with the Pan T-cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol using magnetic activated cell sorting (MACS) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMC using CD4+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions (average purity of 95% ...
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bioRxiv - Immunology 2020Quote: ... splenic T-cells from donor mice were enriched using the Pan T-cell Isolation Kit II (Miltenyi Biotec), while bone marrow (BM ...
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bioRxiv - Cancer Biology 2020Quote: Mouse splenic T cells were isolated from Ptch1+/-:Tp53-/- mice using Pan T cell isolation Kit (Miltenyi Biotec). 4 × 104 mouse T cells were labeled with CFSE (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Pan T cells were enriched through negative selection using magnetic beads (Pan T cell Isolation Kit, Miltenyi Biotec) as suggested by the supplier ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated using magnetic column separation (Miltenyi Biotec, CD4+ T cell isolation kit, human); 17.7 million CD4+ T cells ± 7.2 million were isolated from each subject ...
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bioRxiv - Immunology 2022Quote: ... the mixed T cells were treated with the T cell activation/expansion kit (#130-093-627; Miltenyi Biotec). As controls ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were isolated by MACS using the mouse CD4+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T Cell Isolation Kit was from Miltenyi Biotec Ltd. ...
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bioRxiv - Microbiology 2024Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and were cultured in RPMI medium containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: CD8+/-immune cell fractions were isolated by magnetic-activated cell sorting (130-045-201, CD8 MicroBeads, Miltenyi Biotec). Immune cells were allowed to rest for 24h ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were obtained by pre-enrichment using an “untouched” CD4+ T Cell Isolation Kit (Miltenyi Biotec) with subsequent flow cytometric analysis (CD4+ [clone RM4-5 ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...