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Citations for Miltenyi Biotec :
101 - 150 of 1769 citations for Rat Insulin like growth factor binding protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... ECs cells were labelled with rat anti-mouse CD31 microbeads (Miltenyi Biotec). Labelled ECs and attached cells were then isolated magnetically ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Bioengineering 2024Quote: All samples were blocked for non-specific binding with human FcR Blocking Reagent (Miltenyi Biotec, cat.: 130-059-901) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Murine nCMs were isolated from the ventricles of 1-2 days old wildtype C57BL/6 and Lmnaflox/flox mouse pups using MACS neonatal heart dissociation kit according to the manufacturer (Miltenyi Biotec cat# 130-098-373). Following digestion ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Microbiology 2024Quote: ... 6 μl anti-DYKDDDDK microbeads (Miltenyi Biotec, 130-101-591) were added to 140 μl cell lysates and incubated for 30min on ice ...
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bioRxiv - Immunology 2021Quote: ... and into M2-type macrophages by incubation with 100 ng/ml M-CSF (macrophage-colony stimulating factor, Miltenyi Biotec) in serum-free DC medium (CellGenix ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum and 10 ng/ml granulocyte macrophage colony-stimulating factor (GM-CSF, Miltenyi Biotec) 61 ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in binding buffer after centrifuged and sorted via human CD3 MicroBeads (Miltenyi Biotec, #130-050-101) according to the manufacturer’s recommendation (Note ...
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bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation (IP) was carried out with the μMACS isolation kits for tagged proteins from Miltenyi Biotec (Bergisch-Gladbach, Germany). The eluates ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation of uL18L1-3HA and uL18L8-3HA were performed using the μMACS HA-Tagged Protein Isolation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... GFP-tagged proteins were magnetically labelled and subsequent isolated using µMACS Isolation Kit (Miltenyi Biotec S.L., Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs (hiPSCs) were cultured in a chemically defined growth medium (StemMACS iPS-Brew XF (Miltenyi Biotec) or mTSeRTM1 (Stem Cell Technologies ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... and were allowed to differentiate into Mφs in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF, 20 ng/mL; Miltenyi Biotec) over a 6-day period ...
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bioRxiv - Immunology 2022Quote: ... cells were washed in 0.5% BSA in PBS before fixation/permeabilization with a permeabilization buffer (PB) (Transcription factor staining buffer set, Miltenyi Biotec). After washing ...
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bioRxiv - Molecular Biology 2023Quote: ... or tumor necrosis factor-alpha (1 ng/mL, TNF-α; catalog no. 130-094-022, Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with LPS ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 μL of 1x AnnexinV Binding buffer was added to each tube and cells were analyzed on a MACSQuant VYB (Miltenyi Biotec). A minimum of 10 000 cells were analyzed per condition ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Developmental Biology 2023Quote: ... and maintained undifferentiated in a chemically defined growth medium (StemMACs hiPSC-Brew XF ; MACS Miltenyi Biotec, Paris, France)24 ...
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bioRxiv - Immunology 2020Quote: ... Anti-IL-10 (REA842) and anti-IL-6 (REA1037, Miltenyi Biotec) were used for intracellular staining.
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Immunology 2022Quote: ... Then 1.5x 105 cells/cm2 in 24-well dishes were seeded and mouse receptor activator of nuclear factor kb ligand (RANKL, Miltenyi Biotec, Germany) was added at the final concentration of 35 ng/ml to initiate osteoclasts (OC ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were subsequently stained with IL-17-FITC (1:20, rat, Miltenyi Biotec, 130-102-262), CD-3 APC/cy7 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells from each well was seeded into three or four Matrigel-coated 12-well culture plates and fibroblast growth media was replaced with StemMACS iPS-Brew XF stem cell culture media (Miltenyi Biotec) and changed every day ...
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bioRxiv - Neuroscience 2023Quote: Primary astrocytes were isolated from P0/P1 rat pup cerebral cortices using vendor established protocols (Miltenyi Biotec) and seeded on coated plates (30,000 cells/well in 96 well or 1 million cells/well in 6-well) ...
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bioRxiv - Neuroscience 2023Quote: ... Neural cells were incubated with CD11b+ magnetic microbeads (Miltenyi Biotec, CD11b/c Microbeads, rat, 130-105-634) and CD11b+ cells positively selected through a magnetic column to enrich for microglial cells ...
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation to isolate the protein complexes from total protein extracts using GFP-tagged microbeads (Miltenyi Biotec) has been described previously [8] ...
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bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
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bioRxiv - Immunology 2020Quote: The exosomes extracted from SVV-green fluorescence protein (GFP)-infected cells and normal cells were purified using an exosome purification kit (Miltenyi Biotec, Bergisch Gladbach, Germany), and the copy number of SVV was detected ...
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bioRxiv - Immunology 2020Quote: ... non-pathogenic Th17: 25ng/ml rmIL-6 and 2ng/ml of rhTGFb1 (Miltenyi Biotec); iTreg ...
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bioRxiv - Cell Biology 2021Quote: ... Interacting proteins were isolated by applying total protein extracts to α-GFP-coupled magnetic beads (Miltenyi Biotec). Three replicates of TPLATE motif substitution mutants (SWM and PFM ...
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bioRxiv - Neuroscience 2021Quote: hiPSCs were cultured and maintained undifferentiated in a chemically defined growth media (StemMACS™ iPS-Brew XF medium; Miltenyi Biotec, Paris, France) onto growth-factor-reduced Matrigel™ (Corning)-coated plates (8,6µg/cm2) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:10 50nm-magnetic-bead antibody solution against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523). Nerve fibroblasts express the marker CD90.1 ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μl of 50nm-magnetic-bead antibodies against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523) and incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
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bioRxiv - Neuroscience 2023Quote: ... 2) 4.17 mM GadoSpin-P (n=6; MW=200 kDa; Miltenyi Biotec, Bergisch Gladbach, Germany); and 3 ...
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bioRxiv - Immunology 2023Quote: ... naïve T cells were cultured in the presence of IL-6 (30ng/mL; Miltenyi Biotec), soluble anti-CD28 (1µg/mL ...
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bioRxiv - Systems Biology 2019Quote: Cells were washed once with PBS and stained with CD11b-APC or isotype control (Rat IgG2b-APC, Miltenyi Biotec) antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... Interacting proteins were isolated from total protein extracts using anti-GFP-coupled magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Proteins were digested with trypsin to peptides ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...