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Citations for Miltenyi Biotec :
151 - 198 of 198 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: The fluorescence intensities of the recovered cells were analyzed using a MacsQuant Analyzer flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: ... Neutrophils were counted using an Invitrogen automated cell counter and the purity assessed by flow cytometry using FITC anti-CD16 and APC anti-CD15 antibodies (Miltenyi Biotec) as markers characteristic of human neutrophils.
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bioRxiv - Synthetic Biology 2019Quote: ... Cultures were grown overnight at 37 °C and shaking and then diluted in 1:50 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... All sample volumes were adjusted to 100 µl with 1x PBS + 10 µM ZnCl2 and measured via flow cytometry (MACSquant VYB, Miltenyi Biotec). The flow cytometer was calibrated using MacsQuant calibration beads (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Immunology 2019Quote: ... or sorted using a FACSAria II (Becton Dickinson) flow cytometer after antibody staining and lineage selection or depletion using anti-biotin beads and LS columns (Miltenyi Biotec). Data was analysed using FlowJo software (Version 8.8.7 ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The purity of isolated B cells was over 95% as assessed by flow cytometry analysis of PE-CD19+ cells (Miltenyi Biotec) 87,88.
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bioRxiv - Cancer Biology 2020Quote: ... Pulse-treated cells and non-treated cells harboring a different fluorophore were then mixed in a 50:50 ratio confirmed by flow cytometry (MACSQuant; Miltenyi Biotec). Gates were established using non-fluorescent parental cell lines as a negative control ...
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bioRxiv - Microbiology 2020Quote: ... The fluorescent signal from at least 100,000 individual bacteria per condition was measured by flow cytometry with a MCSQuant® VYB analyzer (Miltenyi Biotec) (Y2 channel ...
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bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples were then diluted 1/1000 in 1xPBS followed by a gently mixing before samples were analyzed by a MACSQuant VYB flow cytometer using filters B1 (525/50nm) and Y2 (615/20nm) (Miltenyi Biotec). Flow rate was adjusted to allow for 1500 events/sec and at least 100 000 events were collected ...
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bioRxiv - Genomics 2020Quote: ... The cell suspension is pipetted through Falcon tubes with cell-strainer caps and then analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec) for 10,000 events.
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... The numbers of cells that migrated to the lower well after 1 hr incubation were counted using a MACSQuant flow cytometer (Miltenyi Biotec). The percent migration was calculated by the numbers of neutrophils that migrated into the bottom chamber divided by the total number of neutrophils in the starting cell suspension and multiplying the results by 100 ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These samples were each added to 200 µL of filtered M9 salt solution inside the wells of a 96 well plate and BFP+ and YFP+ cellular events were quantified by flow cytometry using a Macsquant10 (Miltenyi biotec). The competition plates were then sealed with Breathe-Easier Sealing Film (Diversified Biotech) ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4 °C before being washed in flow cytometry buffer and re-suspended in 100 µl antibody staining cocktail (antibodies and FcR blocking reagent (Miltenyi Biotec) diluted as indicated above) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Pathology 2024Quote: ... and the cells were resuspended in 200 μl of flow cytometry buffer and incubated with 15 μl of human FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: ... 5 % CO2 after which migrated cells in the bottom chamber were collected and acquired on a MACSQuant Analyzer 10 Flow Cytometer using the MACSQuantify software (Miltenyi Biotec). The percentage of specific migration was calculated by dividing the number of cells migrated to the lower well by the total cell input (50 µl cell suspension directly measured on the MACSQuant Flow Cytometer).
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bioRxiv - Neuroscience 2024Quote: ... the only exception being that the flow through from the MACS separation LS column was again loaded on the MACS separation MS column (Miltenyi Biotec) to ensure highest purity of the microglial fraction for TEM imaging ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Genomics 2021Quote: ... The flow through from the column called the CD34- was used for enriching the CD235+ compartment using GP235+ beads (Miltenyi Biotec, Gladbach, Germany). The enriched CD34+ and CD235+ cells were collected ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody staining for FACS was performed in Flow Cytometry Staining Buffer (ThermoFischer Scientific) and following antibodies were used: PE-GLAST (ACSA-1, Miltenyi Biotec, 1/40), APC-CD133 (13A4 ...
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bioRxiv - Cancer Biology 2020Quote: ... wild-type and JAK2V617F mutant marrow Lin-cKit+ HSPCs were isolated by flow sorting and magnetic bead isolation (Miltenyi Biotec, San Diego, CA). Total RNA was extracted using the RNeasy mini kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec, Inc. Cat#130-094-363), CD19 and CD107a (BD Biosciences Cat#555800) ...
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bioRxiv - Genomics 2019Quote: ... Percentage of CD19+ cell was verified by flow cytometry and samples with a purity <70% were purified using magnetic beads according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Extraction of genomic DNA was done using the QIAamp Mini kit according to the manufacturer’s protocol (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... centrifuged and the cell pellet re-dispersed in 100-200 µl of PBS containing 0.5% BSA and the cell fluorescence intensity was measured using a MACSQuant VYB Flow Cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). A minimum of 15 000 events were analyzed for each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... MM cells were pelleted and resuspended in flow cytometry buffer (1× PBS with 1% FBS) then incubated with human FcR blocking reagent (Miltenyi Biotec, # 130-059-901) before staining with diverse fluorescent dye-conjugated monoclonal antibodies ...
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bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... each culture was diluted 100-fold in a new plate containing cold M9-medium before being counted using a MACSQuant10 flow cytometer(Miltenyi biotec, Bergisch Gladbach, Germany). We conducted flow cytometry analysis with a moderate flow rate using SSC 1.4 as a trigger ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Developmental Biology 2023Quote: ... the flow through eluent from microglia selection served either as a cell type fraction depleted of microglia (denoted as “flow through”) or was used for an additional separation between neuronal and glial fractions by using PSA-NCAM antibody (Miltenyi Biotec, 130-092-966) following the same procedure described for microglia purification.
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent intensity of 10 000 cells was detected by flow cytometry on a FACS instrument (MACSQuant®VYB, Miltenyi Biotec, San Diego, CA, USA). Data were analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cancer Biology 2023Quote: ... holoclones and non-holoclones were divided and sorted by flow cytometry for stem-like cells following CD44 staining with CD44 antibody (Miltenyi Biotec Cat no 130-095-180). Sorted CD44high holoclones were used to isolate RNA to quantify stem cell marker expression.