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Citations for Miltenyi Biotec :
601 - 650 of 2996 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... which were then dissociated with a tumor dissociation kit (Human) using a mechanical–chemical–mechanical cycle in a gentleMACS Dissociator (Miltenyi Biotec), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: Immunoaffinity isolation of uEVs was performed using a commercially available kit (Exosome Isolation Kit Pan, human, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of CD81- (for all samples) or CD63- (for MS and HC samples) isolation beads (Exosome Isolation Kit, human Miltenyi Biotec) were added to 2 ml of diluted sample ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec) at an MOI of 1:100 (72 ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... and the cells were resuspended in 200 μl of flow cytometry buffer and incubated with 15 μl of human FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... human (130-050-501) combinations based on the Mini & MidiMACS™ Starting Kit (130-091-632) (all from Miltenyi Biotec, Germany) in accordance with the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were filtered with a 70 μm cell strainer and dead cell removal was performed on single cell suspensions (Miltenyi Biotec) and live tumor cells were frozen in 90% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were isolated by magnetic activated cell sorting (CD14+ Cell Isolation Kit; Miltenyi Biotec). 2×10⁵ monocytes were then differentiated into M1-type macrophages cells by incubation with 1000 U/ml GM-CSF (granulocyte macrophage-colony stimulating factor ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Cancer Biology 2022Quote: ... Red cell lysis and dead cell removal (Miltenyi Biotec) was carried out prior to generation of single cell droplets with 10X Genomics Chromium Controller ...
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bioRxiv - Genomics 2020Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec), and cells were counted again to determine final viable cell numbers.
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bioRxiv - Genomics 2019Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec) and cells were counted again using an automatic cell counter.
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bioRxiv - Cancer Biology 2024Quote: ... the cell suspension was enriched for viable cells using the dead cell removal kit (Miltenyi Biotec) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Human liver parenchymal primary ECs (EC9) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). ant-CD31 was used for sorting of ECs ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP conjugate antibody (Miltenyi Biotec 130-091-833, 1:1000), rabbit anti-golgin-45 (ab155510 ...
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bioRxiv - Genetics 2020Quote: ... NK cell populations using cell-type specific kits (Miltenyi Biotec). For NK cells ...
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bioRxiv - Immunology 2019Quote: Peripheral blood neutrophils from human blood were purified using the Miltenyi Biotech MACSxpress Neutrophil Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, #130-104-434) and residual red blood cells were lysed using MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL, Melbourne, Australia) followed by CD14+ MACS separation (Miltenyi Biotec, Sydney, Australia). The isolated monocytes were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Human tumor tissue samples were acquired during neurosurgical biopsy or open resection and stored in Tissue Storage Solution (Miltenyi Biotec, 130-100-008) for 2-30 hours until further processing for scRadiotracing ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then magnetically enriched for total CD4+ T cells (Stemcell EasySep CD4+ T cell Enrichment kit or Miltenyi Biotec CD4+ T cell Isolation Kit). Enriched CD4+ T cells were stained with surface antibodies for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting of CD45+ cells was performed using the MACS cell separation microbeads and reagents (Miltenyi Biotec). Total RNA was extracted with the RNeasy mini kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec) to lyse blood cells ...
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bioRxiv - Immunology 2022Quote: ... Total aortic CD3+ T-cells and CD3- cells were isolated using the Pan T cell kit (Miltenyi Biotec).
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bioRxiv - Microbiology 2021Quote: ... dead cells were removed from the 1 mL cell suspension using a dead cell removal kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Autologous naïve T cells and NK cells were purified by magnetic-activated cell sorting (MACS®; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension was enriched for live cells using Dead Cell Removal kit (130-090-101; Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’ s protocol before staining for FACS sorting was performed.
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bioRxiv - Immunology 2022Quote: ... dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec). Live cells were resuspended in Advanced DMEM/F-12 medium containing 17.51mM D-glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were seeded 1000 cells/well in organoid medium in 384-well plates (PrimeSurface® ...
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bioRxiv - Immunology 2022Quote: ... the cells were filtered using a 30µm cell strainer (Miltenyi Biotec), and the Dead Cell Removal Kit was used to remove dead cells to obtain a cell viability of ≥80%.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were first depleted for CD45+ cells (Miltenyi Biotec, SanDiego, CA) and then positively selected for CD31+ cells (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were mixed with 1% alginate (ratio of 1:1 ...
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bioRxiv - Immunology 2022Quote: ... followed by dead cell removal (Dead Cell Removal Kit, Miltenyi Biotec). NK cells were stained with CellTrace Violet as indicated above ...
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bioRxiv - Immunology 2022Quote: ... Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit ...