Labshake search
Citations for Miltenyi Biotec :
101 - 150 of 185 citations for Orexin B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... we purified B cells (Fig. 1D) by positive selection using magnetic beads (MACS, Miltenyi Biotec) conjugated with a monoclonal anti-mouse B220 antibody (clone ...
-
bioRxiv - Immunology 2019Quote: ... PBMCs were isolated from 16 healthy individuals and enriched for CD19+ B cells (Miltenyi Biotec). Two times 2.5 × 105 of naive B cells (CD20+IgM+IgD+IgG− ...
-
bioRxiv - Immunology 2021Quote: ... B cells were excluded from whole splenocytes by incubating with anti-CD19 beads (Miltenyi Biotec) or by using the EasySep™ mouse CD19 positive selection kit (STEMCELL Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: B cells were isolated from cryopreserved PBMCs by positive selection using CD19+ microbeads (Miltenyi Biotec) and expanded through CD40-CD40L stimulation by culturing cells for 4-5 days with irradiated NIH3T3 feeder cells constitutively expressing CD40L at 37°C in 5% CO2 in B cell medium ...
-
bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
-
bioRxiv - Immunology 2023Quote: ... The cells were subsequently processed using the Pan B Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2019Quote: Splenic CD4+ T cells or CD19+ B cells were positively selected using immunomagnetic MicroBeads (Miltenyi Biotec) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: ... B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec). Cells were washed twice with 5 ml FACS buffer with 3 mM EDTA and adjusted to a cell density of 1-2×106 cells/mL ...
-
bioRxiv - Immunology 2020Quote: ... B cells were purified with CD43 micro beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany), and 1×106 cells were placed in the top chamber of a transwell plate in 100μL of volume ...
-
bioRxiv - Microbiology 2021Quote: ... and B-cells were affinity-purified using CD19 microbeads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) resulting in >95% pure B-cell populations ...
-
bioRxiv - Immunology 2022Quote: ... B cells were first isolated from donors’ PBMC by MACS using human CD19 MicroBeads (Miltenyi Biotec). CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes ...
-
bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
-
bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
-
bioRxiv - Immunology 2020Quote: ... was added to B cells and then analyzed by flow cytometry (MacsQuant Analyzer 10; Miltenyi Biotec,). The double labeling procedure allows a distinction into living (Annexin V −/PI−) ...
-
bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
-
bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
-
bioRxiv - Immunology 2022Quote: ... using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
-
bioRxiv - Microbiology 2024Quote: CD19+ B cells were purified by positive magnetic separation using CD19 MicroBeads (Miltenyi Biotec, Gaithersburg, MD) and MS or LS columns ...
-
bioRxiv - Microbiology 2019Quote: ... CD19 microbeads were used for magnetic separation of purified B cells using an autoMACS separator (Miltenyi Biotec). All cell lines in this study were cultured according to standard mammalian tissue culture protocols (ATCC ...
-
bioRxiv - Immunology 2021Quote: ... CD19+ B cells were purified from human PBMCs using the REAlease® CD19 Microbead Kit (Miltenyi Biotec) according to the manufacturer’s recommendations with purity greater than 98 %.
-
bioRxiv - Immunology 2019Quote: ... untouched B cells were enriched using anti-CD43 magnetic beads (MACS) according to manufacturer’s protocol (Miltenyi Biotec) obtaining > 95 % purity ...
-
bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
-
bioRxiv - Immunology 2023Quote: ... B lymphocytes were enriched from erythrocyte-lysed spleen cells using CD43 MicroBeads (Miltenyi Biotec,130-049-801) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Immunology 2023Quote: B cells were purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec) and subsequently expanded for 10-14 days in RPMI-1640 supplemented with 10% human serum (Bavarian Red Cross) ...
-
bioRxiv - Bioengineering 2024Quote: ... B cells were neutralized with Ca2+/Mg2+ free DPBS with 0.5% BSA (Miltenyi Biotec, 130-091-376) and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: ... Naïve mature B cells were isolated from spleen cells by immunomagnetic negative selection with anti-CD43 (Miltenyi Biotec). B cells were cultured in RPMI-1640 medium (Corning ...
-
bioRxiv - Cancer Biology 2020Quote: For CSR experiments spleen naive B cells were isolated by immunomagnetic depletion using anti-CD43 beads (Miltenyi Biotec). Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco) ...
-
bioRxiv - Cancer Biology 2022Quote: ... CD19+ B cells were magnetically removed from freshly isolated PBMCs using CD19 Microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s recommendations (depleted condition) ...
-
bioRxiv - Molecular Biology 2021Quote: Nave B lymphocytes were purified from mouse spleens by negative selection using CD43 (Ly48) magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions. ...
-
bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was achieved using the 37_C Multi_tissue_dissociation B program on the gentleMACS octo-dissociator (Miltenyi Biotec). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: ... Total splenic B cells (CD19+) were purified using CD19 MicroBeads (130-121-301, Miltenyi Biotec, San Jose, CA), and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862 ...
-
bioRxiv - Cell Biology 2022Quote: ... and B cells and CD8+ T cells were isolated from these using the human CD19 Microbeads (Miltenyi Biotec) or the human CD8+ isolation kit (Miltenyi Biotec).
-
bioRxiv - Molecular Biology 2023Quote: Splenic naïve B-cells were prepared using the CD43 negative selection commercial kit (Miltenyi Biotec.; 130-090-862). Spleens were pulled from 2-3 sacrificed sex- and age- matched animals mechanically disintegrated and filtered through a 70 µm cell strainer to prepare a single cell suspension essential for microbead separation ...
-
bioRxiv - Immunology 2023Quote: ... and B cells were isolated by negative selection directly from whole blood using MACSxpress® separator (Miltenyi Biotec) with Whole Blood B Cell Isolation Kit (Miltenyi Biotec) ...
-
bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... CD3+ T lymphocytes and CD20+ B lymphocytes were sorted from PBMCs using CD3+ and CD20+ microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
-
bioRxiv - Genetics 2022Quote: ... Peripheral T- and B-cells were isolated from whole spleen using Pan T Cell (Miltenyi Biotec,130-095-130) or Pan B Cell (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: Naïve B cells (CD43-B220+CD3-) were obtained by negative selection (mouse CD43 Ly-48 MicroBeads; MACS Miltenyi Biotec) from spleens of WT and Lrba-/- mice with 95% purity ...
-
bioRxiv - Immunology 2019Quote: ... Either PBMC or B cells magnetically sorted using the AutoMACS Pro cell separator and CD19+ microbeads (both Miltenyi Biotec), were lysed in RLT buffer (Qiagen) ...
-
bioRxiv - Immunology 2021Quote: ... B cells were first isolated by magnetic cell sorting using the human CD19 MicroBeads (Miltenyi Biotec, San Diego, CA) and the AutoMACS magnetic cell separator ...
-
bioRxiv - Immunology 2022Quote: ... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...
-
bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were filtered to remove dead cells and B-cell depletion was performed using human CD19 microbead (Miltenyi Biotec) labeling according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
-
bioRxiv - Immunology 2023Quote: We prepared B cell tetramers of biotinylated S2hlx-Ex19 or FP-10 by mixing with Streptavidin-VB515 (Miltenyi Biotec) and Streptavidin-AlexaFluor 647 (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: Splenic B cells of both genotypes were CD43-depleted using magnetic beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany) and taken in culture in the presence of 1μg/ml anti-CD40 antibody (102901 ...
-
bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...