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Citations for Miltenyi Biotec :
51 - 100 of 130 citations for Native Red Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
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bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent intensity of 10 000 cells was detected by flow cytometry on a FACS instrument (MACSQuant®VYB, Miltenyi Biotec, San Diego, CA, USA). Data were analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... RBCs and debris of dead cells in single cell suspensions were respectively removed using Red Blood Cell Lysis Solution (10x) Kit (Miltenyi Biotec, Germany) and Dead Cell Removal Kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Cell Biology 2023Quote: PRP from human subjects was subjected to white blood cell (WBC) and red blood cell (RBC) depletion by using CD45 MicroBeads (for WBCs, #130-045-801, Miltenyi Biotec., Gaithersburg, MD) and CD235a (Glycophorin A ...
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bioRxiv - Immunology 2020Quote: ... and spike proteins (Prot_S) were purchased from Miltenyi Biotec. Optimal peptides for the manufacture of HLA class I tetramers were synthesized at >95% purity by Peptides & Elephants GmbH ...
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bioRxiv - Cell Biology 2019Quote: The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... the same method was followed with the substitution of the fluorescent antibody mix against tetraspanins (CD63, CD81 and CD9) with the APC labelled HLA-G antibody (130111852, Miltenyi Biotec, same volume of 15 µl). This method ensured that EVs attached to the Ab conjugated beads were only detected if positive for HLA-G.
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic cell dissociation and debris and red blood cell removal were performed using the Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes and CD19+ B cells were enriched from fresh PBMCs derived from blood donors (Swiss Red Cross Laboratory; ECCT: CE-3428) through positive immunoselection (130-050-201 and 130-050-301, respectively [Miltenyi Biotec, Bergisch Gladbach, Germany]) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were eluted with 100 μL elution buffer (Miltenyi Biotec). Proteins were resolved in 8% SDS-PAGE gels described above.
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bioRxiv - Immunology 2020Quote: Protein A beads and µ columns were from Miltenyi Biotec Ltd. ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was incubated with RBC lysis buffer for 5 min on ice to remove red blood cells (RBC) and then used for Treg isolation with a CD4+CD25+ Treg isolation kit (Miltenyi Biotec, cat # 130-091-041), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were harvested by centrifugation at 400g for 10 min and resuspended in chilled 1X Red Blood Cell Removal Solution (Miltenyi Biotec, cat. no.130-094-183). Debris was then removed using the Debris Removal Solution (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2019Quote: ... Co-immunoprecipitated proteins were isolated using magnetic μMACS columns (Miltenyi Biotec) as previously described61 ...
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bioRxiv - Microbiology 2022Quote: ... then 50 μL of protein G conjugated nanobeads (Miltenyi Biotec, Germany) were added on samples ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were eluted off the beads with elution buffer (Miltenyi Biotec). Proteins were resolved using SDS polyacrylamide gel electrophoresis and gels were sent to the Rutgers Biological Mass Spectrometry Facility ...
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bioRxiv - Plant Biology 2023Quote: ... Protein-DNA complexes were immunoprecipitated using anti-HA antibodies (Miltenyi Biotec) (Kaufmann et al. ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were detected with α-GFP-HRP (Miltenyi Biotec, 130-091-833), α-Myc-HRP (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... and 100 μL of μMACSTM Protein A/G MicroBeads (Miltenyi Biotec, Germany) were added for 1 hr incubation before applying to pre-equilibrated μ columns ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike PepTivator® protein pools (Miltenyi Biotec, Gladbach, GER) were used to test T cell responses against full spike proteome ...
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bioRxiv - Plant Biology 2019Quote: ... immuno-purification was performed with µMACS GFP Tag Protein Isolation kits (Miltenyi Biotec). 1 mg of solubilized microsomes were loaded on µcolumns ...
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bioRxiv - Plant Biology 2022Quote: ... μMACS™ GFP or μMACS™ DYKDDDDK (FLAG) Protein Isolation Kits (Miltenyi Biotec) were used for immunoprecipitations ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 µM rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec) for the first 24 h after passaging ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation for 2.5 h at 4 °C while slowly rotating ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Immunology 2022Quote: ... or S-protein (B.1.351) mutation peptide pool (Miltenyi Biotec, # 130-127-958, Germany) at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were captured with 100µl of μMACS protein A beads (Miltenyi Biotec; San Diego, CA). Beads were isolated with μ columns and a μMACS separator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000; Miltenyi Biotec) for one hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: RNA immunoprecipitation (RIP) assays were performed with the μMACS GFP-Tagged Protein Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Plant Biology 2020Quote: ... Eluted proteins were analysed by western blot using HRP-conjugated anti-myc antibody (130-092-113; Miltenyi Biotec), or an anti-HA antibody (3F10 ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Immunology 2020Quote: ... The splenocytes were incubated with the spike protein peptide pool at 1.5 μg/ml/peptide (Miltenyi Biotec, Germany) at 37 °C for 16-18 hours ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation (IP) was carried out with the μMACS isolation kits for tagged proteins from Miltenyi Biotec (Bergisch-Gladbach, Germany). The eluates ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation of uL18L1-3HA and uL18L8-3HA were performed using the μMACS HA-Tagged Protein Isolation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Developmental Biology 2021Quote: ... GFP-tagged proteins were magnetically labelled and subsequent isolated using µMACS Isolation Kit (Miltenyi Biotec S.L., Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...