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Citations for Miltenyi Biotec :
151 - 200 of 1863 citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... WT mouse by depleting T cells from the spleen using the CD3ε microbead kit according to the manufacturer’s instructions (Miltenyi Biotec). T cells were stained with CellTrace Violet as described in the above section and plated in 96-well flat-bottom plates at 1.5×105 T cells per well with/without 2×104 antigen presenting cells isolated from naïve WT spleen ...
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bioRxiv - Cell Biology 2020Quote: ... Lineage positive cells were depleted using a mouse lineage cell depletion kit (Miltenyi Biotec, 130-090-858, 130-110-470) and the following antibodies from BioLegend CD150 (Cat#115913 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from spleen of P2Y6fl/fl and P2Y6ΔNcr1 mice fed a normal chow diet using the mouse NK cell isolation kit from Miltenyi Biotec, (Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator treatment following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 24h ...
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from mouse spleen and lymph nodes by magnetic bead sorting using the Naïve CD4+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... mouse (Miltenyi Biotec, Germany). Lung homogenates were filtered through a 70 μM cell strainer ...
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bioRxiv - Microbiology 2023Quote: ... mouse (Miltenyi Biotec, Germany). Lung homogenates were filtered through a 70 μM cell strainer ...
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bioRxiv - Immunology 2021Quote: ... NK cells in the spleen were isolated and purified by using mouse NK cell isolation kit II (130-096-892, Miltenyi Biotec). The purity of NK cells was analyzed by FACS ...
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bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... femurs of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Afterwards ...
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bioRxiv - Cancer Biology 2020Quote: ... multiple femur of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... from control and CL- and cold-treated mice were collected after CL treatment and processed for SVC isolation using mouse adipose tissue dissociation kit (Miltenyi biotec) according to manufactures.
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany). Pairs of liver samples from the same experimental group were pooled and subjected to a second round of purification ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Cancer Biology 2021Quote: ... live CD45− cells were isolated by negative selection from digested WT and CCN4-KO tumors using the mouse CD45 (TIL) MicroBeads kit (Miltenyi Biotec). A total of 1 ×104 cells/well were cultured for 36 h in DMEM supplemented with 10% dialyzed FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T-cells were isolated from lymph nodes and spleens of OT-II TCR transgenic mice using negative selection with a mouse CD4+ T Cell Isolation Kit (Miltenyi Biotec). Splenocytes from C57BL/6 mice were T cell depleted by complement lysis of Thy1.2+ cells (clone J1J ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor pieces were dissociated using a modified protocol based on Miltenyi (mouse) Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... from control and treated mice were collected and processed for isolation of stromal vascular cells (SVC) using the mouse adipose tissue dissociation kit (Miltenyi Biotec) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... PDOX-derived cells were FACS-sorted (P8, nude control brain)23 or MACS-purified (remaining PDOXs) with a Mouse Cell Depletion kit (Miltenyi Biotec)21 ...
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bioRxiv - Cancer Biology 2023Quote: Mouse tumors were excised and ∼200Lmg of tumor tissue was enzymatically and mechanically digested using the mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single-cell suspension ...
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bioRxiv - Immunology 2023Quote: ... 1×106 vehicle or ACCi-conditioned OT-1 T cells were infused into B16-OVA tumor bearing mice 10 days after tumor challenge and tumors were harvested 7 days later using the Mouse Tumor Dissociation Kit and gentleMACS Octo dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were then purified by negative magnetic cell sorting using mouse CD8α+ T-cell isolation kit (130-104-075; Miltenyi Biotec) according to the manufacture’s protocols ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... gentle and rapid generation of single-cell suspensions was achieved by using a modified version of the procedure of a mouse Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2023Quote: CD4 T cells were isolated from the spleens of donor mice and purified using the mouse CD4 T Cell Isolation Kit (Miltenyi Biotec). The purity and cell count of donor CD4 T cells were assessed using flow cytometry ...