Labshake search
Citations for Miltenyi Biotec :
351 - 400 of 500 citations for Mouse NRAP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...
-
bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
-
bioRxiv - Immunology 2019Quote: ... WT mouse by depleting T cells from the spleen using the CD3ε microbead kit according to the manufacturer’s instructions (Miltenyi Biotec). T cells were stained with CellTrace Violet as described in the above section and plated in 96-well flat-bottom plates at 1.5×105 T cells per well with/without 2×104 antigen presenting cells isolated from naïve WT spleen ...
-
bioRxiv - Cell Biology 2020Quote: ... Lineage positive cells were depleted using a mouse lineage cell depletion kit (Miltenyi Biotec, 130-090-858, 130-110-470) and the following antibodies from BioLegend CD150 (Cat#115913 ...
-
bioRxiv - Immunology 2020Quote: ... splenocytes were incubated on ice for 20 minutes with B220 microbeads (Miltenyi Biotec,; 20 μL/1000 μL SM per mouse), washed with SM and enriched on an AutoMACS Pro ...
-
bioRxiv - Immunology 2021Quote: ... NK cells were isolated from spleen of P2Y6fl/fl and P2Y6ΔNcr1 mice fed a normal chow diet using the mouse NK cell isolation kit from Miltenyi Biotec, (Bergisch Gladbach ...
-
bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
-
bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
-
bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
-
bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
-
bioRxiv - Microbiology 2023Quote: ... and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator treatment following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
-
bioRxiv - Immunology 2023Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 24h ...
-
bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from mouse spleen and lymph nodes by magnetic bead sorting using the Naïve CD4+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s protocol ...
-
bioRxiv - Pathology 2024Quote: ... The cells were resuspended in 200 μl of PBS containing 0.5% BSA and 2 mM EDTA and incubated with 15 μl of mouse FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
-
bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
-
bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
-
bioRxiv - Immunology 2021Quote: ... NK cells in the spleen were isolated and purified by using mouse NK cell isolation kit II (130-096-892, Miltenyi Biotec). The purity of NK cells was analyzed by FACS ...
-
bioRxiv - Immunology 2021Quote: ... and purified their T cells using the untouched isolation kit (Pan T Cell Isolation Kit II, mouse, 130-095-130, Miltenyi Biotec).
-
bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
-
bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... Each mouse was sacrificed at day 17 to collect feces and intestinal sections all homogenized in PBS using gentleMACSTM OCtoDissociator (Miltenyi Biotec) and plated on both Drigalski plates and LB plates overlayed with strain Mt1B1 ...
-
bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
-
bioRxiv - Cancer Biology 2020Quote: ... After two weeks of treatment the mice were euthanized and the lungs were dissociated according to the kit instructions (Mouse Lung Dissociation Kit, MACS Miltenyi Biotec) and the cells were sorted for the GFP positive fraction ...
-
bioRxiv - Physiology 2019Quote: ... Resulting stromal vascular fractions were used to isolate ATMs using mouse anti-F4/80-FITC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-FITC MicroBeads (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
-
bioRxiv - Immunology 2019Quote: 2 × 105 peritoneal exudates cells were stained with fluorochrome-conjugated antibodies (anti-mouse) directed against the T-cell subset surface marker CD4 (Biotin, Miltenyi Biotec), the alpha chain of the IL-2 receptor CD25 (PE ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
-
bioRxiv - Cell Biology 2019Quote: ... For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770, 130-104-224, Miltenyi Biotec), CD105 (APC ...
-
bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... femurs of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Afterwards ...
-
bioRxiv - Cancer Biology 2020Quote: ... multiple femur of engrafted mice were combined for each condition and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2020Quote: ... from control and CL- and cold-treated mice were collected after CL treatment and processed for SVC isolation using mouse adipose tissue dissociation kit (Miltenyi biotec) according to manufactures.
-
bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
-
bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
-
bioRxiv - Genetics 2022Quote: ... kidneys were quickly dissected after mouse euthanization and transferred to gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in 2ml of digestion buffer (13.5 ml of Dispase (STEMCELL ...
-
bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed and CD11c+ dendritic cells were enriched using CD11c MicroBeads UltraPure (mouse) (Miltenyi Biotec, #130-125-835).
-
bioRxiv - Cancer Biology 2022Quote: ... Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany). Pairs of liver samples from the same experimental group were pooled and subjected to a second round of purification ...
-
bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
-
bioRxiv - Cancer Biology 2021Quote: ... live CD45− cells were isolated by negative selection from digested WT and CCN4-KO tumors using the mouse CD45 (TIL) MicroBeads kit (Miltenyi Biotec). A total of 1 ×104 cells/well were cultured for 36 h in DMEM supplemented with 10% dialyzed FBS (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
-
bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... whole bone marrow (WBM) cells isolated by crushing femur and tibia from each mouse were incubated with 1x RBC Lysis Solution (Miltenyi Biotec) to eliminate red blood cells ...
-
bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Cell Biology 2020Quote: ... CD133 expression was detected by anti-CD133–phycoerythrin (AC133-PE) antibody or PE-conjugated mouse immunoglobulin (Ig)G1 isotype control antibody (Miltenyi Biotec). The expression of Sox2 was analyzed by PerCP-Cy 5.5 mouse anti-Sox2 or PerCP-Cy 5.5 mouse IgG1 isotype control (Becton Dickinson [BD]) ...
-
bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...