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Citations for Miltenyi Biotec :
201 - 250 of 1907 citations for Mouse Anti Human IgG Fc Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+ monocytes were collected in the resuspended cell suspension by incubating with anti-human CD14 microbeads (Miltenyi Biotec) for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were stained with anti-human CD34 (AC136, APC, Miltenyi Biotec, Cat No 130-113-738, 1:50) and anti-human CD38 (IB6 ...
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bioRxiv - Bioengineering 2023Quote: ... the following antibodies were used depending on the experiment: anti-human ROR1 APC (130-118-015; Miltenyi Biotec), anti-human CD31 FITC (130-110-806 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated on ice with an Fc block reagent (Miltenyi Biotec) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc receptors were blocked using Fcr Blocking Reagent (Miltenyi Biotec, 130-059-901) according to manufacter’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Fc block was performed using FcR blocking reagent (Miltenyi Biotec, 130-092-575). Staining buffer consisted of PBS with 2 mM EDTA and 0.5% BSA ...
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bioRxiv - Immunology 2022Quote: ... The samples were preincubated with anti-mouse CD16/CD32 (Miltenyi Biotec, 130-092-574) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... together with PE-conjugated mouse anti-GLAST 1:15 (Miltenyi Biotec 130-095-821) for 15 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... diluted 1:2000 (v/v) or anti-GFP HRP-coupled (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Immunology 2021Quote: ... and 10 μl of APC-conjugated anti-mouse F4/80 (Miltenyi Biotec, CA, USA) for 30 min at 4 °C in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse CD45-FITC (1:50, #130-116-500, Miltenyi Biotec, Auburn, CA, USA) and NIR for exclusion of dead cells (1:103 ...
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bioRxiv - Pathology 2024Quote: ... followed by incubation with anti-mouse FcR blocking reagent (130-092-575, Miltenyi Biotec) in PBS 2% FBS for 10 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and mouse anti-GFP HRP-conjugated at 1:5,000 (130-091-833, Miltenyi Biotec). Chemiluminescence detection was conducted using SuperSignal West Femto Maximum Sensitivity Substrate (34096 ...
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bioRxiv - Immunology 2024Quote: Resting B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Primary resting mouse splenic B-cells were isolated using anti-CD43 microbeads (Miltenyi Biotec) and activated with 25 ug/mL LPS (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human CD34-PE (Miltenyi Biotec);and Human CD33-APC-Cy7 (Abcam).
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bioRxiv - Immunology 2024Quote: ... human FcR Blocking (Miltenyi Biotec), and 5% mouse serum (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and MACSiBead Particles pre-coated with anti-human CD335 (NKp46) and CD2 antibodies based on manufacturer guidelines (Miltenyi Biotec). Primary adult CD34+ HSCs were cultured at 0.5 x 106 cells per mL in SFEMII medium supplemented with CC110 cytokine cocktail (StemCell).
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... with 20% FCS or a whole skin digestion kit from Miltenyi Biotec (Cambridge, U.K.). Single cell suspensions were obtained and subsequently stained with the following antibodies against ...
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bioRxiv - Microbiology 2023Quote: ... Recovered cells were incubated with Fc block (Miltenyi Biotec, Bergusch Gladbach, Germany, 1:100), vortexed ...
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bioRxiv - Immunology 2020Quote: ... and IgG APC IS11-3B2.2.3 (Miltenyi Biotec). Data of labelled cells were acquired using a Beckman Coulter Gallios instrument and FlowJo ™ software (BD ...
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Systems Biology 2019Quote: ... cell suspensions were stained with anti-mouse CD45-PE-Vio770 (Miltenyi Biotec, 130-110-661), CD326-BV421 (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812, MACS Miltenyi Biotec), 0.14 µg/mL PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Immunology 2021Quote: Lung epithelial cells separated by magnetic bead: the anti-mouse CD45 magnetic bead (Miltenyi Biotec) was used to enrich the epithelial cells in single-cell suspension ...
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bioRxiv - Immunology 2022Quote: ... mouse splenocytes were subjected to ACK lysis followed by anti-CD4 positive selection (Miltenyi Biotec). Naive CD4+ T cells (CD4+CD25-CD44-CD62L+ ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Immunology 2021Quote: ... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... human” (Miltenyi Biotec, Bergisch Gladbach, Germany) following the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... human (Miltenyi Biotec, Bergisch Gladbach, Germany)].
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bioRxiv - Cell Biology 2022Quote: ... human (Miltenyi Biotec, 130-107-086). 1 μg/ml puromycin (Sigma ...
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bioRxiv - Genomics 2022Quote: ... human (Miltenyi Biotec #130-094-131). Unstimulated naïve CD4+ T cells were cultured in IMDM (Gibco ...
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bioRxiv - Immunology 2019Quote: ... Human FcR-Block (Miltenyi Biotec, Germany) was included to reduce unspecific staining ...
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bioRxiv - Cell Biology 2020Quote: ... human (130-091-379, Miltenyi Biotec), from the negative fraction of the CD14-positive fraction.
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bioRxiv - Cell Biology 2020Quote: ... human (130-050-201, Miltenyi Biotec). The positive fraction was used as CD14-positive monocytes ...
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bioRxiv - Immunology 2022Quote: ... human positive selection kit (Miltenyi Biotec) and total RNA was extracted from hCD45+ cells using NucleoSpin® RNA kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... human (Miltenyi Biotec, 130-095-929). Removal of debris and red blood cells was performed for tumor resections as described above ...