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Citations for Miltenyi Biotec :
501 - 550 of 625 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 1 mL PEB buffer and proceeded to magnetic separation on autoMACSpro separator (Miltenyi Biotec) with Possels program ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked at 37 C for 15 min with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec). A stimulation with an equimolar amount of DMSO was performed as negative control ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were blocked at 37°C for 15 min with 0.5 μg/ml anti-CD40 mAb (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2022Quote: ... The RPMI medium was additionally supplemented with 200 ng/mL human Flt3 ligand (Miltenyi Biotec, 130-096-479) and 5 ng/mL murine GM-CSF (Peprotech ...
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bioRxiv - Microbiology 2021Quote: ... dead cells were removed from the 1 mL cell suspension using a dead cell removal kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The splenocytes were incubated with the spike protein peptide pool at 1.5 μg/ml/peptide (Miltenyi Biotec, Germany) at 37 °C for 16-18 hours ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were stimulated with SARS-CoV-2 peptide pools of S and N (2 μg/ml) (Miltenyi Biotec) in the presence or absence of CECs ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Microbiology 2020Quote: ... was homogenized in 1 ml PBS for 2 × 55 sec on a gentleMACS™ Octo Dissociator (Miltenyi Biotec) using a C-Tube (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... lungs were harvested in 2 ml of Trizol and homogenized using a gentleMACS Octo dissociator (Miltenyi Biotec, Inc.). Total RNA was purified with trizol/chloroform ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 and 4 cytokines were added as followed: 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 100 ng/ml GM-CSF (#130-093-864 ...
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bioRxiv - Cancer Biology 2021Quote: ... in 5 mL of IMDM+Glutamax medium for 30 min at 37°C in gentleMACS C tubes (Miltenyi Biotec). During and after incubation ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Immunology 2020Quote: ... PBMCs or purified NK cells were stimulated for 16-18 hours with 10 ng/mL rhIL-12 (Miltenyi Biotec), 100 ng/mL rhIL-15 (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... IFN-β and IFN-γ (final conc. was 50 ng/mL for 30 min; Miltenyi Biotec, Bergisch Gladbach, Germany), TNF-α (final conc ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum and 10 ng/ml granulocyte macrophage colony-stimulating factor (GM-CSF, Miltenyi Biotec) 61 ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then pelleted and resuspended in 5 mL PBSF and sorted using a MidiMACS Separator magnet (Miltenyi Biotec) in combination with an LS column (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5 ml of extract was incubated with 50 µl of either Anti-Myc (Miltenyi Biotec, ref. 130-091-123) or Anti-HA (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... 5 mL of FACS buffer was added and the samples were passed over a magnetized LS column (Miltenyi Biotec). The tube and column were washed once with 5 mL FACS buffer and then removed from the magnetic field ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Immunology 2022Quote: ... 5 mL of FACS buffer was added and the samples were passed over a magnetized LS column (Miltenyi Biotec). The tube and column were washed once with 5 mL FACS buffer and then removed from the magnetic field ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was removed and 1 mL of wash buffer (PBS + 0.5% BSA (Miltenyi Biotec, Cat. # 130-091-376) + 1 U/uL of RNase inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended in 100 µl cold D-PBS and stained with fluorochrome-conjugated antibodies recognizing human CD11b and GFAP (Miltenyi Biotec, 130-113-810 and 130-123-846) as described above ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Spleens were collected and homogenized in PBS supplemented with 2.5 mg/ml saponine using the gentleMACS homogenizer with gentleMACS M tubes from Miltenyi Biotec. Amastigotes were purified as previously described [21] for DNA extraction ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... and were allowed to differentiate into Mφs in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF, 20 ng/mL; Miltenyi Biotec) over a 6-day period ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Genetics 2023Quote: ... and the precipitated pellet was resuspended in 2.5 mL 1× Buffer and filtered with a 70 µM MACS SmartStrainer (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Molecular Biology 2023Quote: ... or tumor necrosis factor-alpha (1 ng/mL, TNF-α; catalog no. 130-094-022, Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with LPS ...
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bioRxiv - Immunology 2024Quote: ... resuspended at 4 mio cells/ml and then cultured with HLA class I-enhanced neurons (see Human-iPSC-derived neuron preparation section above) at a density of 1.2mio cells/cm2 with IL-2 (1000UI/ml, Miltenyi Biotec) and a CD28 agonist antibody (5ug/ml ...
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bioRxiv - Immunology 2024Quote: ... CD8+ L1CAM-CAR T cells (used for in vitro experiments) were generated and expanded as previously described (16) and supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 50U/ml IL2 (Novartis) ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
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bioRxiv - Immunology 2019Quote: HIV-1-infected or mock-infected CD8-depleted PBMCs cells were used as targets and autologous cryo-preserved PBMCs rested overnight in R10 supplemented with 10ng/ml of IL-15 (Miltenyi Biotec) were used as a source of effector cells ...
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bioRxiv - Cancer Biology 2019Quote: ... whole lungs from mice bearing MDA-LM2 macrometastases (3 weeks post tail vein injection) were harvested and lysed in 1 ml Cell Lysis Buffer 2 (R&D) using the gentleMACS Dissociator (Miltenyi Biotec). As a control ...
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
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bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Microbiology 2020Quote: ... One gram of each tissue was homogenized in 9 ml of PBS in gentleMACS M tubes using the appropriate setting on the gentleMACS dissociator (Miltenyi Biotec). Homogenates were filtered through 40-μm-pore-size filters and an aliquot was used to determine viable counts ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...