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Citations for Miltenyi Biotec :
351 - 400 of 611 citations for L Phenylalanine N T Boc 13C9 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... CD3 and CD28 monoclonal antibodies (beads T cell activation/Expansion Kit, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) were co-cultured with WJ-MSCs (A/ WJ hMSCs ...
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bioRxiv - Immunology 2021Quote: ... pooled spleen and/or lymph nodes were prepared utilizing the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and purified by negative selection through an LS column (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were enriched by negative selection on a LD column and QuadroMACS separator (Miltenyi Biotec), before positive selection of γδ T cells using MS columns with an OctoMacs separator (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2022Quote: ... and resting CD4+ T cells were isolated with LS magnetic separator columns (Miltenyi Biotec, #130-096-533). Subsequently ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were positively sorted with beads from the spleens of C57BL/6 mice (Miltenyi Biotec). The T cells were labelled with CFSE (Invitrogen Molecular Probes) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were sorted from PBMCs using magnetic bead-based cell sorting CD8 MicroBeads (Miltenyi Biotec), the process was performed according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched via Magnetic-activated Cell Sorting (MACS) using CD4 (L3T4) microbeads (Miltenyi Biotec). In some instances ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were positively stained using CD8a (Ly-2) microbeads (Miltenyi Biotec, cat. # 130-117-044) and the negative flow-through fraction was collected ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... and CD8 T cells from dLNs were purified using a magnetic bead separation kit (Miltenyi Biotec, Germany). 106 CD8 T cells were injected into the lesions of CD45.1 mice ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec, Bergisch Gladbach, Germany) using the “CD4+ T Cell Isolation Kit II” from Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... cells were rested overnight (o/n) at 37°C in TexMACS Medium (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 10% AB serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from differently treated mice were isolated using CD4+ microbeads according to manufacturer’s instruction (Miltenyi Biotec). The purity of CD4+ T cells was analyzed by flow cytometry after the staining with anti-CD4 antibody and reported to be > 95% ...
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bioRxiv - Cancer Biology 2020Quote: CD3+T-cells were isolated from healthy C57/B6 mice using a Miltenyi kit (Miltenyi Biotec, Auburn, CA). Round bottom plates were coated with 10μg of functional CD3 antibody (eBiosciences ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from the spleen and peripheral lymph nodes using anti-CD4 microbeads (Miltenyi Biotec) following the manufacture’s protocol ...
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bioRxiv - Immunology 2022Quote: ... both naïve and memory OT-1 CD8 T cell donors were magnetically sorted via magnetic beads (Miltenyi Biotec) with specific antibodies ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by negative selection from spleens and lymph nodes (Miltenyi Biotec, #130-104-454) according to manufacturer’s instructions (typically >95% purity) ...
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bioRxiv - Genomics 2022Quote: ... brain tissue was processed using a MACS Neural Tissue Dissociation Kit (T) (Miltenyi Biotec, Cat#: 130-093-231) and manually dissected according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... fresh splenocytes were isolated as previously described and subjected to Pan-T cell magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were enriched from the spleen cells by using mouse CD4 MACS Beads (L3T4, Miltenyi Biotec) and then CD4+CD44loCD62LhighFoxp3-eGFP- naïve T cells were purified by sorting with flow cytometry (FACS Aria III ...
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bioRxiv - Immunology 2020Quote: ... and T cells were depleted using Thy1.1 or Thy1.2 positive selection and a magnetic separation kit (Miltenyi Biotec). Cells were counted ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
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bioRxiv - Immunology 2021Quote: Naïve CD4 T cells were isolated from mice spleens and lymph nodes by magnetic bead separation (Miltenyi Biotec) following the manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... Autologous naïve T cells and NK cells were purified by magnetic-activated cell sorting (MACS®; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and B cells and CD8+ T cells were isolated from these using the human CD19 Microbeads (Miltenyi Biotec) or the human CD8+ isolation kit (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... EGFRt-positive T cells were enriched using biotin-conjugated anti-EGFR mAb and anti-biotin beads (Miltenyi Biotec), and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Circulating CD8+ T lymphocytes were isolated from fresh heparin blood by negative selection using magnetic beads (Miltenyi Biotec) from PBMC ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8 T cells were isolated from the LNs of NY 8.3 NOD mice using two rounds of the naïve 8.3 CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated and expanded with the T Cell Activation/Expansion Kit (Miltenyi Biotec, Cat No. 130-091-441) according to the vendor’s manual ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... the enriched CD8+ T cells were stimulated with the CMVpp65 overlapping peptide pool PepTivator® CMVpp65 (Miltenyi Biotec) at a concentration of 1 µg of each peptide/ml for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Cat #130-104-075). Rag1-/- mice were reconstituted with CD8+ T cells by intravenous route (3 x 106 cells/mouse ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T lymphocytes and CD20+ B lymphocytes were sorted from PBMCs using CD3+ and CD20+ microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were then separated from the white cells using mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Purity of CD4+ T cell population was measured by flow cytometry (MACSquant Analyzer using MACSQuantify software, Miltenyi Biotec), showing a fraction of at least 95% CD4+CD3+ double positive cells and less than 0.01% CD123+CD304+CD303+ cells ...
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bioRxiv - Microbiology 2024Quote: Primary CD4+ T cells were purified from PBMCs of a healthy donor by negative selection (Miltenyi Biotec, USA). Purified CD4+ T cells were stimulated with 1 µg/mL soluble anti-CD3 (clone OKT3 ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant was incubated with 50 L magnetic mMACS anti-DYKDDDDK Microbeads (Miltenyi Biotec) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD8+ T cells were purified from the spleen of OT-I.Ly5.1 mice using CD8a+ T Cell Isolation Kit II (Miltenyi Biotec). OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched from spleen with CD4 microbeads (Clone: L3-T4, Miltenyi Biotec, San Diego, CA, USA). The enriched CD4+ T cells were stained with antibodies to define TFH cells as described in flow methods below ...
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bioRxiv - Immunology 2021Quote: Primary murine naïve CD4 T cells were isolated from the spleens and lymph nodes of SLE1.2.3 or control mice using the CD4+CD62L+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). CD62L positive selection was routinely ∼98% in purity as determined by flow cytometry ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens by using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... CD8+ T cells were isolated by negative selection using magnetic beads following the instructions from the manufacturer (Miltenyi Biotec). The purified CD8+ T cells were incubated with APC-conjugated HLA-A2/YFV NS4b (LLWNGPMAV ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Germany), resuspended at 106 cells/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive selection was performed to isolate CD8+ T cells from primary tumors and spleens using CD8a microbeads (Miltenyi Biotec), an LS column ...