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Citations for Miltenyi Biotec :
251 - 300 of 696 citations for IL 7 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... we assessed the capacity of NK cells to perform ADCC after being stimulated with IFN-α or IL-15 (Miltenyi Biotec). In such cases ...
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bioRxiv - Immunology 2020Quote: ... DC differentiation was carried out over 6 days with 250 U/ml IL-4 and 50ng/ml GM-CSF (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
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bioRxiv - Immunology 2020Quote: ... The adherent monocytes were then washed twice with PBS and resuspended in RPMI supplemented with the cytokines GM-CSF (1000 U/mL) and IL-4 (500 U/mL) (Miltenyi Biotec). Differentiation to immature MDDCs was achieved by incubation at 37 °C with 5% CO2 for 7 days and subsequent activation with cytokines addition every other day ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured with 100 ng/mL (500 UI/mL) GM-CSF plus 50 ng/mL (250 UI/mL) IL-4 (Miltenyi Biotec) at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2023Quote: ... adjusted at 5×105 cells/ml and cultured 2-5 additional days in cell culture medium supplemented with 20 ng/ml IL-2 (Miltenyi Biotec) before being used in the experiments.
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bioRxiv - Cell Biology 2024Quote: ... to serve as a control (i.e., unstimulated cells) or interleukin- 1β (IL-1β; 50ng/mL; Miltenyi Biotec, Cat#130-101-682) plus interferon-γ (IFNγ ...
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bioRxiv - Bioengineering 2024Quote: ... rested overnight at a density of 1×107 cells/mL in medium supplemented with 100 IU/mL IL-2 (Miltenyi Biotec). On day 7 ...
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bioRxiv - Bioengineering 2024Quote: ... and rested overnight in a density of 1×107 cells/mL in medium supplemented with 100 IU/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2022Quote: ... Mouse hepatic parenchymal cells were digested and non-parenchymal cells were isolated by Mouse Liver Dissociation Kit (Miltenyi Biotec). Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Germany), resuspended at 106 cells/ml ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Systems Biology 2021Quote: ... these mice were euthanized and the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (130-104-075, Miltenyi Biotec), resuspended in a concentration of 106/ml ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Cancer Biology 2023Quote: Tumor samples harvested from mouse mammary glands were dissociated using the Mouse Tumor Dissociation Kit (Miltenyi Biotec,130-096-730), gentleMACS C tubes (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Mouse FRCs required sorting using MACS (Miltenyi Biotec), with anti-mouse CD45 and CD31 magnetic beads ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse IgG1-APC (Order no:130092214, Miltenyi Biotec), Mouse Anti-human CD34 (CD34-APC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mouse and rat (Miltenyi Biotec 130-098-373) at 37 °C with gentle pipetting ...
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bioRxiv - Immunology 2022Quote: ... were minced using scissors in RPMI media containing enzyme cocktail mix from Miltenyi Biotec mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA). Tumor pieces were further digested as per manufacturer’s instructions and digested tissue was filtered through 70μm strainer to obtain a single cell suspension ...
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bioRxiv - Immunology 2021Quote: ... mouse (Miltenyi Biotec Cat No. 130-095-927) and GentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... mouse and rat (Miltenyi Biotec, 130-107-677) was used for adult mouse brain dissociation of WT mice and contralateral hemispheres of glioblastoma mice ...
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bioRxiv - Neuroscience 2023Quote: ... mouse and rat (Miltenyi Biotec, 130-107-677) was used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... human and mouse (Miltenyi Biotec, 130-049-601) and a MACS separation system (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse FcR blocker (Miltenyi Biotec 130-092-575), added to a base blocking buffer consisting of 10% normal goat serum ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-mouse CD117-PE(Miltenyi Biotec, #REA791) antibody was used ...
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bioRxiv - Bioengineering 2024Quote: ... and mouse (Miltenyi Biotec, cat.: 130-092-575) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Immunology 2020Quote: ... and the remaining adherent monocytes were cultured for 48h in the presence of IL-4 (300 U/ml) and GM-CSF (450 U/ml) (both from Miltenyi Biotec, Madrid, Spain) in X-VIVO 15 (BioWhittaker ...
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bioRxiv - Immunology 2020Quote: ... Ly6G+ cells were isolated by Neutrophil isolation Kit mouse and F4/80+ cells by Anti-F4/80 Microbeads Ultrapure mouse kit followed the manufactory instructions (MACS, Miltenyi Biotec).
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Neuroscience 2019Quote: ... Contaminating mouse splenocytes were depleted from cell preparations by incubating with MACS anti-mouse CD45 magnetic microbeads (Miltenyi Biotec, Auburn, CA) and then washing through magnetic LD Columns as per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Media was replenished every 48 hours for 7 days at which point BMDCs were harvested and magnetically selected using CD11c specific microbeads (Miltenyi Biotec, Cat# 130-125-835).
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Genetics 2019Quote: ... with 300 IU/mL mouse IFN-α (Miltenyi Biotec) for 15 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and vioblue-labelled anti-mouse CD69 (Miltenyi Biotec, Germany) were used for identification of cell sub-populations and activation status ...
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bioRxiv - Immunology 2022Quote: ... We used mouse lung (Miltenyi Biotec; 130-095-927) and spleen dissociation kits (Miltenyi Biotec ...