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Citations for Miltenyi Biotec :
501 - 550 of 764 citations for IL 4 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were purified from mouse bone marrow using anti-Ly6G MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2022Quote: ... Muscle stem cells (MuSCs) were isolated from mouse TA muscle using a satellite cell isolation kit (Miltenyi Biotec) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit, Miltenyi Biotec, Auburn, CA, USA), using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... single-cell suspensions prepared as above were depleted of CD45 positive cells using mouse CD45 microbeads (Miltenyi Biotec) prior to staining ...
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bioRxiv - Cancer Biology 2020Quote: ... the femur samples were combined and murine cells were depleted using the Mouse cell depletion kit (Miltenyi Biotec). Cells were stained for 15min at 4°C and then transferred to LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... Thy1.2 cells were depleted via negative selection using the CD90.2 MicroBeads mouse Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions from whole mouse lungs were prepared using the lung dissociation kit (Miltenyi Biotec, Auburn, CA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). Purity checks and cell counts were performed using a BD Accuri C6 flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... and stained with 100 μl PE-conjugated mouse anti-human IgA (1:40; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... MuSCs were selected using the Satellite Cell Isolation Kit mouse (130-104-268, Miltenyi Biotec, Bergisch Gladbach, Germany), seeded at 2000 cells/cm2 and grown for 3 days in growth medium (GM ...
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bioRxiv - Cancer Biology 2020Quote: ... MACS-based purification was performed with Myelin Removal Beads II followed by Mouse Cell Depletion kit (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: Mouse splenic T cells were isolated from Ptch1+/-:Tp53-/- mice using Pan T cell isolation Kit (Miltenyi Biotec). 4 × 104 mouse T cells were labeled with CFSE (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Immunology 2020Quote: Mouse monoclonal antibodies against human CD86 (clone FM95) and DC-SIGN (clone DCN47.5) were purchased from Miltenyi Biotec. Antibodies against human CD83 (clone HB15e ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were enriched from the spleen cells by using mouse CD4 MACS Beads (L3T4, Miltenyi Biotec) and then CD4+CD44loCD62LhighFoxp3-eGFP- naïve T cells were purified by sorting with flow cytometry (FACS Aria III ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...
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bioRxiv - Molecular Biology 2021Quote: Nave B lymphocytes were purified from mouse spleens by negative selection using CD43 (Ly48) magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions. ...
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bioRxiv - Immunology 2021Quote: ... BM progenitor cells were purified from each suspension using the Lineage Cell Depletion Mouse Kit microbeads (Miltenyi Biotec). Magnetic separation was performed with an autoMACS separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:10 50nm-magnetic-bead antibody solution against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523). Nerve fibroblasts express the marker CD90.1 ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were isolated by MACS using the mouse CD4+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The brain (minus cerebellum and olfactory bulb) was dissociated using the adult mouse brain dissociation kit (Miltenyi Biotec). The tissue ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μl of 50nm-magnetic-bead antibodies against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523) and incubated for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... GCB cells were first enriched from mouse splenocytes using the PNA MicroBead Kit (Miltenyi Biotec, 130-110-479), followed by antibody incubation and sorting on BD Aria II sorter (RRID:SCR_018091 ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
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bioRxiv - Neuroscience 2023Quote: OPCs were isolated from p7 mouse brains using magnetic activated cell sorting and anti-NG2 MicroBeads (Miltenyi Biotec). Tissue dissection and cell sorting were performed under sterile conditions ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: Single-cell suspensions were prepared from mouse lungs using the Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Genomics 2024Quote: ... tissues from each mouse were transferred to a chilled gentleMACS C Tube (Miltenyi Biotec cat. #130-093-237) with 2 mL Nuclei Extraction Buffer (Miltenyi Biotec cat ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were then separated from the white cells using mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), according to the manufacturer’s protocol ...