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Citations for Miltenyi Biotec :
1 - 50 of 62 citations for ICOS Rhesus Macaque HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... rhesus PBMC were combined with magnetic CD4 Microbeads (Miltenyi Biotec) and processed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
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bioRxiv - Immunology 2021Quote: ... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
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bioRxiv - Immunology 2021Quote: NK cell effectors were enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion Kit (Miltenyi Biotec). Target and effector cells were plated at E:T ratio of 2:1 in a 96 well round bottom plate ...
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bioRxiv - Developmental Biology 2021Quote: ... Rhesus fetal lung tissue (~500 mg/tube) was cut into small pieces and transferred into a gentleMACS C-tube (Miltenyi Biotec, 130-093-237) containing 5 mL of digestion buffer (1 mL of Dispase [Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... Fc receptor block (Miltenyi Biotec Inc) was added and incubated on ice-water bath for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Fc receptor blocker (Miltenyi Biotec Inc) was added and ice-water bath was used for 10 minutes ...
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bioRxiv - Immunology 2019Quote: ... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively.
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bioRxiv - Immunology 2024Quote: ... containing Fc receptor blocker (Miltenyi Biotec) and incubated for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... human Fc blocking reagent (MACS Miltenyi Biotec) was added to this blocking step ...
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bioRxiv - Cancer Biology 2022Quote: ... the Fc receptor was blocked (Miltenyi Biotec) and cells were stained with the following antibodies for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated with Fc blocking reagent (Miltenyi Biotec) for 10 minutes at room temperature before the addition of monoclonal antibodies for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in Fc receptor blocker (Miltenyi Biotec) diluted in PBS/2% BSA and incubated on ice for 10 min ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with Fc receptor blocker (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (v/v) containing FcR blocking reagent (Miltenyi Biotec) with fluorescently labeled antibodies as indicated in table 2 and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
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bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
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bioRxiv - Cancer Biology 2020Quote: ... and blocked using Fc Receptor block (Miltenyi Biotec 130-092-575). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... Nonspecific binding was blocked by using 1% Fc block (Miltenyi Biotec). Cells were stained with HLA-A2 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in human FC block (Miltenyi Biotec Inc., FLP3330) and kept on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4C with anti-human Fc Block (Miltenyi Biotec). Cells were incubated with DAPI viability dye (Thermo ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated in ice with Fc block reagent (Miltenyi Biotec) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and blocked with PBS supplemented with 1% Fc Block (MACS, Miltenyi Biotec) and 0.5% BSA for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... and Fc-receptors were blocked by the mouse FcR Blocking Reagent (Miltenyi Biotec)(0.5μL/well ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated on ice with an Fc block reagent (Miltenyi Biotec) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc receptors were blocked using Fcr Blocking Reagent (Miltenyi Biotec, 130-059-901) according to manufacter’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Cells were blocked with mouse Fc receptor block (Miltenyi Biotec, San Diego, CA) and stained with conjugated primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Fc block was performed using FcR blocking reagent (Miltenyi Biotec, 130-092-575). Staining buffer consisted of PBS with 2 mM EDTA and 0.5% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
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bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
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bioRxiv - Immunology 2020Quote: ... with 20% FCS or a whole skin digestion kit from Miltenyi Biotec (Cambridge, U.K.). Single cell suspensions were obtained and subsequently stained with the following antibodies against ...
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bioRxiv - Microbiology 2023Quote: ... Recovered cells were incubated with Fc block (Miltenyi Biotec, Bergusch Gladbach, Germany, 1:100), vortexed ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cell Biology 2021Quote: ... either MNCs or CD34 enriched cells were thawed and washed and stained with FC block (Miltenyi Biotec) for 10 min and washed again ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was performed on 500µl blood samples from healthy donors with or without Fc fragment receptor blockers (Miltenyi Biotec). Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...