Labshake search
Citations for Miltenyi Biotec :
351 - 400 of 569 citations for Human RPP25L shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... CD14+ monocytes were collected in the resuspended cell suspension by incubating with anti-human CD14 microbeads (Miltenyi Biotec) for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... CD4 T cells were purified by negative selection using the human CD4 T Cell Isolation Kit (Miltenyi Biotec). T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 12.5 μL Enzyme A (all provided in the human multi-tissues dissociation kit (Miltenyi Biotec; 130-110-201), and 2.35 mL DMEM (Gibco ...
-
bioRxiv - Cancer Biology 2022Quote: ... Freshly isolated PDX tumors were dissociated into single-cell suspension using a Human Tumor Dissociation Kit (Miltenyi Biotec) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec) ...
-
bioRxiv - Microbiology 2022Quote: ... and stained with 100 μl PE-conjugated mouse anti-human IgA (1:40; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
-
bioRxiv - Neuroscience 2022Quote: Day 37 neurons were harvested using the Miltenyi MACS human mitochondria isolation kit (Miltenyi Biotec 130-094-532) with additional proteases added to the lysis buffer aprotinin (10 μg/ml) ...
-
bioRxiv - Immunology 2022Quote: ... The RPMI medium was additionally supplemented with 200 ng/mL human Flt3 ligand (Miltenyi Biotec, 130-096-479) and 5 ng/mL murine GM-CSF (Peprotech ...
-
bioRxiv - Bioengineering 2019Quote: ... Patient-derived GBM6 and GBM12 cells were stained with human-CD133 (PE conjugated 1:11 dilution; Miltenyi Biotec), then with human-CD68 (FITC conjugated 1:20 dilution ...
-
bioRxiv - Immunology 2020Quote: Mouse monoclonal antibodies against human CD86 (clone FM95) and DC-SIGN (clone DCN47.5) were purchased from Miltenyi Biotec. Antibodies against human CD83 (clone HB15e ...
-
bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the CD56+CD3− NK cells were isolated by using the human NK cell isolation kit (Miltenyi Biotec) following the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2021Quote: ... The CB cells were lineage depleted with the human lineage cell depletion kit (Miltenyi Biotec, 130-092-211), which contains the following biotin-conjugated monoclonal antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were stained with anti-human CD34 (AC136, APC, Miltenyi Biotec, Cat No 130-113-738, 1:50) and anti-human CD38 (IB6 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Leland Chung) and PC3-mm (RRID: CVCL_4885) were blocked with human FcR blocking reagent (1:100; Miltenyi Biotec) and subsequently incubated with anti-CD117/c-kit-APC (1:50 ...
-
bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
-
bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
-
bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated using magnetic column separation (Miltenyi Biotec, CD4+ T cell isolation kit, human); 17.7 million CD4+ T cells ± 7.2 million were isolated from each subject ...
-
bioRxiv - Cell Biology 2022Quote: ... and B cells and CD8+ T cells were isolated from these using the human CD19 Microbeads (Miltenyi Biotec) or the human CD8+ isolation kit (Miltenyi Biotec).
-
bioRxiv - Cancer Biology 2022Quote: ... For tumor samples digestion was performed using tumor dissociation kit for human (130-095-929, MACS Miltenyi Biotec), mechanical dissociation using gentleMACSTM Dissociator running the gentleMACS program h_tumor_01 ...
-
bioRxiv - Biophysics 2022Quote: ... and REA control (I)-APC human for the control of unspecific binding (Miltenyi Biotec, Ref. 130-104- 615). At least 10000 events were gated according to the FSC/SSC dot plot with the exclusion of dead cells.
-
bioRxiv - Immunology 2023Quote: ... according to the manufacturer’s suggestions and monocytes purified using positive selection with paramagnetic CD14 (human) MicroBeads (Miltenyi Biotec). CD14+ monocytes were differentiated into macrophages using 100 ng/mL of recombinant human M-CSF (Immunotools ...
-
Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... monocytes were isolated from the PBMCs using a monocyte isolation kit (Pan Monocyte Isolation Kit, human, Miltenyi Biotec). Next ...
-
bioRxiv - Cancer Biology 2023Quote: ... and subsequently enriched for CD34+ cells using the human CD34 MicroBead kit (130-046-702 MACS Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... the following antibodies were used depending on the experiment: anti-human ROR1 APC (130-118-015; Miltenyi Biotec), anti-human CD31 FITC (130-110-806 ...
-
bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
-
bioRxiv - Immunology 2023Quote: ... pDCs were enriched from freshly isolated PBMCs using the human Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec) following manufacturer’s instructions with a 10% reduction in the recommended amounts of reagents ...
-
bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMC) were purified from whole blood by density gradient centrifugation (FicollPaqueTM, Miltenyi Biotec). Whole blood was collected from healthy donors through an NIH Department of Transfusion Medicine (DTM)-approved protocol (Institutional Review Board of the National Institute of Allergy and Infectious Diseases [NIAID]) ...
-
bioRxiv - Immunology 2023Quote: ... explants were digested using the Human Tumour Dissociation Kit with the gentleMACS Octo Dissociator program 37C_h_TDK_1 (Miltenyi Biotec), as per the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and MACSiBead Particles pre-coated with anti-human CD335 (NKp46) and CD2 antibodies based on manufacturer guidelines (Miltenyi Biotec). Primary adult CD34+ HSCs were cultured at 0.5 x 106 cells per mL in SFEMII medium supplemented with CC110 cytokine cocktail (StemCell).
-
bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec) and published protocols 50,51 ...
-
bioRxiv - Immunology 2021Quote: ... Tumors were minced and processed following the instructions for the human tumor dissociation kit (Miltenyi Biotec, 130-095-929). After the addition of enzyme mix in 5 ml null RPMI ...
-
bioRxiv - Molecular Biology 2020Quote: EV were depleted from citrated plasma using the exosome isolation kit pan human kit (130-110-912, Miltenyi Biotec) directed against a combination of CD9 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
-
bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2022Quote: EV samples were tested with a bead-based multiplex analysis (MACSPlex exosome kit, human, Miltenyi Biotec, Bergisch Galdbach, Germany). EV samples were diluted with MACSPlex buffer to a final volume of 120 µl with 5.8×108 EVs per sample ...
-
bioRxiv - Microbiology 2022Quote: ... PE anti-human IgA-stained bacteria were incubated with Anti-PE Magnetic Activated Cell Sorting (MACS) beads (Miltenyi Biotec) (1:5 ...
-
bioRxiv - Cancer Biology 2022Quote: Fresh tumor and matched normal samples were dissociated into single cell suspension using human tumor dissociation kit (Miltenyi Biotec) and gentleMACS™ Dissociator (Miltenyi Biotec) ...
-
bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
-
bioRxiv - Immunology 2021Quote: ... Human recombinant interleukin-7 (IL-7) and interleukin-15 (IL-15) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Retronectin (Recombinant Human Fibronectin ...
-
bioRxiv - Immunology 2019Quote: Single cell suspensions of PBMCs and CD15-depleted SF cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and labeled for 15 min at 4 °C with antibodies for fluorescent activated cell sorting (FACS ...
-
bioRxiv - Immunology 2019Quote: Human neutrophils were isolated by magnetic separation using MACSxpress whole blood neutrophil isolation kit (Miltenyi Biotec, 130-104-434) and were seeded into a micro-insert 4 well dish (Ibidi 80406) ...
-
bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
-
bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... The remaining tissue was further minced with scissors and enzymatically dissociated using the Human Tumour Dissociation Kit (Miltenyi Biotec) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... The naïve T cells were purified from human PBMC using Pan T cell isolation kit (Miltenyi Biotec, Cat#130096535) and activated and expanded by CD3/CD28 Dynabeads (GibcoTM ...
-
bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
-
bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
-
bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...