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Citations for Miltenyi Biotec :
601 - 650 of 2373 citations for Human Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
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bioRxiv - Plant Biology 2020Quote: ... the μMACS c-myc Isolation Kit and μMACS HA Isolation Kit (Miltenyi Biotec) were used according to the instructions provided ...
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bioRxiv - Neuroscience 2023Quote: ... The cerebral cortex was cleaned of meninges and the entire cell populations were isolated by enzymatic digestion and mechanical dissociation using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, Bergisch Gladbach, GER) according to manufacturer instructions followed by a 30% density gradient centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Biochemistry 2019Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec). The isolated monocytes were differentiated for 7 days in IMDM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: Human iPSC/ESCs (Table S1) were maintained on dishes coated with Matrigel in mTeSR1 or StemMACS iPS-Brew XF (Miltenyi Biotec) media with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Monocyte isolation was performed by gradient centrifugation on Percoll (Pharmacia, Uppsala, Sweden) following by magnetic-activated cell separation sorting using human CD14 Microbeads (Miltenyi Biotec). Freshly isolated CD14+ monocytes were cultured for 7-8 days to differentiate into macrophages in IMDM medium supplemented with 10% FCS ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25- cells were then obtained by depletion of CD25+ T cells using CD25 positive selection (CD25 microbeads II, Human, Miltenyi Biotec) and collecting the unlabeled cells.
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bioRxiv - Immunology 2023Quote: ... Cells were magnetically labeled and separated according to the instructions of CD45 MicroBeads Human (order no. 130-045-801, Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Day 12 CD34+ hematopoietic cells were purified via magnetic-activated cell sorting (MACS) using human CD34 microbeads (#130-046-702, Miltenyi Biotec). As previously reported [48] ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched using human anti-CD8 MicroBeads and the automated magnetic activated cell sorting (autoMACS) system (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... and the cells were resuspended in 200 μl of flow cytometry buffer and incubated with 15 μl of human FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... and dissociation kit (Miltenyi Biotec) following the machine dissociation protocols ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cut along the coronal plane with a razor into three pieces and digested into cell suspensions using a Multi Tissue Dissociation kit 1 (Miltenyi Biotec, Germany; #130-110-201) and a gentleMACS™ OctoDissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were extracted with the CD117 Microbead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were cultured in complete StemPro-34 serum-free medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... and -Ter119) or c-kit enrichment was performed using MACS magnetic microbead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Human ES cells were maintained at 37°C in StemMACS(tm) iPS-Brew XF media (Miltenyi Biotec GmbH, cat#130-104-368), supplemented with penicillin ...
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bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected for TSDR demethylation analysis and stored at −80 as cell pellets or expanded with 500-1000 U/mL human rIL2 (Miltenyi Biotec/Chiron) +/− 100 nM rapamycin (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Pre-enrichment for c-kit+ cells was achieved using anti-c-kit magnetic beads (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... an Inside Stain kit (Miltenyi Biotec) was used according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The Neuron Isolation Kit (Miltenyi Biotec) was used for magnetic selection of neuronal cells.
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bioRxiv - Pathology 2020Quote: ... Dead Cell Removal Kit (Miltenyi Biotec) was used to remove the cells with low viability ...
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bioRxiv - Molecular Biology 2022Quote: ... and Treg Expansion kit (Miltenyi Biotec) which contains beads pre-loaded with anti-CD3 and anti-CD28 antibodies.
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bioRxiv - Immunology 2021Quote: MACSPLEX Cytokine 10 kit (Miltenyi Biotec) was used for the Cytokine bead array following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mouse lung dissociation kit (Miltenyi Biotec) was used with some additional modifications ...
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bioRxiv - Neuroscience 2023Quote: ... dead cell removal kit (Miltenyi Biotec) to purify single cells ...
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bioRxiv - Neuroscience 2022Quote: ... the Neuron isolation kit (Miltenyi Biotec) was used according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Insideperm (Inside Stain Kit, Miltenyi Biotec) was used as permeabilizer and antibody preparation ...
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bioRxiv - Genomics 2023Quote: ... and Mitochondria Isolation Kit (Miltenyi Biotec), per the manufacturer’s Extraction Kit protocol ...