Labshake search
Citations for Miltenyi Biotec :
551 - 600 of 1891 citations for Human IgG1 Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... CD4+ T were purified using magnetic beads coated with anti-CD4 antibody (clone L3T4) according to the manufacturer’s instructions (Miltenyi Biotec). 5×105 2D2 CD4+ T cells were injected i.v ...
-
bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
-
bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
-
bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 ng/mL Fibroblast Growth Factor 2 (FGF-2) (Miltenyi Biotec, 130-105-787), for 14 days with medium change every 2 days ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
-
bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
-
bioRxiv - Physiology 2019Quote: ... Resulting stromal vascular fractions were used to isolate ATMs using mouse anti-F4/80-FITC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-FITC MicroBeads (Miltenyi Biotec ...
-
bioRxiv - Immunology 2019Quote: 2 × 105 peritoneal exudates cells were stained with fluorochrome-conjugated antibodies (anti-mouse) directed against the T-cell subset surface marker CD4 (Biotin, Miltenyi Biotec), the alpha chain of the IL-2 receptor CD25 (PE ...
-
bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
-
bioRxiv - Microbiology 2020Quote: ... BMDCs were enriched by depletion of BMMs using biotinylated α-F4/80 antibody (eBioscience, RRID: AB_466657) and anti-biotin conjugated magnetic beads (Miltenyi Biotec). The BMDCs in the flow through were positively selected using α-CD11c magnetic beads according to the manufacturer’s protocol (Mil405 tenyi Biotec).
-
bioRxiv - Immunology 2019Quote: ... or sorted using a FACSAria II (Becton Dickinson) flow cytometer after antibody staining and lineage selection or depletion using anti-biotin beads and LS columns (Miltenyi Biotec). Data was analysed using FlowJo software (Version 8.8.7 ...
-
bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
-
bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
-
bioRxiv - Immunology 2020Quote: ... For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34 ...
-
bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
-
bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
-
bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
-
bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
-
bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed twice with FACS Intracellular Staining Perm Wash buffer and the following antibodies were added to the cells: anti-STAT1 pTyr701 (Miltenyi Biotec), and anti-STAT3 pTyr705 (eBioscience™) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
-
bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101, Miltenyi Biotec). CD4+ T cell enrichment was confirmed by determining the percentage of CD3+/CD4+ cells via flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
-
bioRxiv - Immunology 2024Quote: ... CD4+CD25- Tcon were isolated from the CD25- fraction obtained from the same samples by negative selection using FITC-conjugated antibodies and anti-FITC Microbeads (Miltenyi Biotec) as described above (T cell isolation) ...
-
bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
-
bioRxiv - Immunology 2021Quote: ... IL-2 Catch Reagent (Miltenyi Biotec), IL-4 Catch Reagent (Miltenyi Biotec ...
-
bioRxiv - Bioengineering 2023Quote: ... such as IWP-2 (Miltenyi Biotec). On day 5 ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 1X human FcR blocker (stock: 50x Mac’s Miltenyi Biotec 130-059-901). After washing ...
-
bioRxiv - Microbiology 2020Quote: ... and monocytes were isolated using human CD14 MicroBeads (130-050-201, Miltenyi Biotec) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Human primary monocytes were isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA) from peripheral blood mononuclear cells obtained from healthy human donors (de-identified ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
-
bioRxiv - Neuroscience 2022Quote: ... human BDNF (50 µL, 100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (25 µL ...
-
bioRxiv - Cell Biology 2021Quote: ... Human NP tissue samples were transported in MACS Tissue Storage Solution (Miltenyi Biotec) immediately after surgical resection ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples for surface staining were incubated with human FcR Blocking Reagent (Miltenyi Biotec) together with PE-conjugated mouse anti-GLAST 1:15 (Miltenyi Biotec 130-095-821 ...
-
bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec) and activated with T Cell TransAct (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2021Quote: ... Human CD14 microbeads were used in combination with LS columns (both Miltenyi Biotec) to positively select CD14+ blood monocytes ...
-
bioRxiv - Immunology 2021Quote: ... T cells were selected using human CD4 microbeads and MS columns (Miltenyi Biotec). Procedure was carried out according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... recombinant human Interferon gamma1b (IFNγ1b) was purchased from Miltenyi Biotec (130-096-481). Exon1-mHTT97Q-eGFP and Exon1-wtHTT25Q-eGFP cloned in pcDNA3.1 plasmids were kindly provided by Dr ...
-
bioRxiv - Biophysics 2023Quote: ... Monocytes were enriched from human PBMC by CD14 beads positive selection (Miltenyi Biotec) and cultured in IMDM supplanted with 10% human AB serum (omega scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... PBMCs were then labelled with the human NK Cell Isolation Kit (Miltenyi Biotec), with subsequent NK cell isolation using the “depletes” program on an autoMACS Pro Separator and collecting the negative fraction ...
-
bioRxiv - Synthetic Biology 2023Quote: ... T cells were activated with TransAct Human T cell activation reagent (Miltenyi Biotec) and 100 U/ml human IL-2 IS (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 50 IU/ml premium-grade human IL2 (Miltenyi Biotec, 130-097-746) at 1x106 cells per mL and cm2 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...