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Citations for Miltenyi Biotec :
251 - 300 of 1068 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Spleens were placed in 3 ml of PBS supplemented with 2% FBS in C tubes and dissociated using program m_spleen_01 on a gentleMACS Octo dissociator with heaters (Miltenyi Biotec). Dissociated tissue was passaged through a 70 μm cell strainer and centrifuged at 1500 rpm x 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue pieces (up to 500 mg) were transferred into a gentleMACS C tube (130-096-334, Miltenyi Biotec) containing 1,950 μL of enzyme mix 1 (enzyme P and buffer Z) ...
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bioRxiv - Cell Biology 2019Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
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bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
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bioRxiv - Neuroscience 2020Quote: ... in C-tubes (Miltenyi, 130-096-334) on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). Afterwards ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were digested using the Neural Tissue Digest Kit-P in C-tubes as per manufactures directions (Miltenyi Biotec). Two brains were digested in each C-tube using program 37_ABDK on the GentleMACS™ OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Tumor and lymph node samples were thoroughly minced with surgical scissors and transferred to GentleMACs C Tubes (Miltenyi Biotec) containing 800 U/ml Collagenase IV (Worthington ...
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bioRxiv - Immunology 2022Quote: ... Digested tumors were then transferred to GentleMACs C Tube and homogenized using the Gentle MACs Octo Dissociator (Miltenyi Biotec) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue and digestion buffer were transferred to a gentleMACS C Tube (run protocol 37C_h_TDK_1) (Miltenyi Biotec, 130-096-334) and incubated at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Physiology 2023Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... transferred in gentleMACS C-tubes containing RPMI/DMEM media and kit enzymes (tumor dissociation kit, Miltenyi Biotec, Gladbach, Germany) and were broken down using the gentleMACS ...
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bioRxiv - Neuroscience 2023Quote: ... Hemispheres were cut into 8-12 small pieces and placed into gentleMACS C-tubes (Miltenyi Biotec, #130-093-237) with dissociation solution prepared as instructed by the manufacturer’s protocol ...
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... at 4°C for 10 minutes and then isolated using MACs LS (Miltenyi Biotec; Catalog no. 130-042-401) columns following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL of μMACS anti-GFP or anti-c-Myc (Miltenyi Biotec, 130-091-125 and 130-091-123) was added to clarified extract and incubated at 4 °C while rotating for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... Digested tumors were then transferred to GentleMACs C Tube and homogenized using the Gentle MACs Octo Dissociator (Miltenyi Biotec) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Cancer Biology 2021Quote: ... After incubation each sample was placed in 9ml ice-cold 0.04% BSA/PBS in a C-type GentleMacs tube (Miltenyi Biotec). Tissue was further dissociated using a GentleMACs dissociator with programs A1 and two rounds of program B1 with the sample kept on ice between programs ...
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bioRxiv - Cancer Biology 2020Quote: Tumors were dissected and minced following the manufacturer’s protocol using MACS C tubes and the mouse Tumor Dissociation Kit (Miltenyi Biotec). After tumor dissociation ...
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bioRxiv - Immunology 2019Quote: ... Bone marrow cells were MACS enriched for c-Kit+ cells using CD117 MicroBeads Ultrapure (Miltenyi Biotec cat #130-091-224) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Half of the livers were processed to enrich lymphocytes according to manufacturer’s instructions with a gentleMACS Dissociator in C tubes (Miltenyi Biotec). Alternatively ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec). CD45+ cells were isolated using magnetic beads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Biochemistry 2021Quote: ... with an incubation time of 3h at room temperature and using secondary labels 0.6 µL anti-c-myc-FITC (Miltenyi Biotec) and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82) ...
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bioRxiv - Neuroscience 2022Quote: ... Up to 1.25 grams of cortical tissue were placed in each gentleMACS™ C Tube (Miltenyi Biotec, # 130-093-237) which contained 5 mL of 0.3 mg/mL papain (Worthington ...
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bioRxiv - Neuroscience 2022Quote: ... The gentleMACS™ C Tubes were placed on the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, # 130-096-427) with heating cuffs attached and underwent the gentleMACS Program 37C_ABDK_01 protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2020Quote: ... for 30 minutes at 37°C and dissociated through a 30-mm pore cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany). For chemokine binding assays ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2021Quote: ... the samples were gently incubated with a proprietary enzymatic solution in C-tubes (Miltenyi Biotec, Cat. No.: 130-096-334) at 37°C for at least 30 minutes using a rotator ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Dissociated kidneys were incubated for 30 minutes at 37 °C with continuous rotation using the MACSmix Tube Rotator (Miltenyi Biotec). GentleMACS C tubes were then placed in the gentleMACS Dissociator and Multi_E_02 program was run ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Pathology 2023Quote: ... In short small pieces of full-thickness specimens were placed in a gentleMACS C-tube (130-093-237, Miltenyi Biotec) in digestion solution and dissociated in the gentleMACS Octo Dissociator (130-095-937 ...
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bioRxiv - Systems Biology 2024Quote: ... tumor was cut into 1-2 mm pieces and transferred to a gentleMACS C Tube (Miltenyi Biotec #130-093-237) containing 4.7 mL of sterile media (Knockout DMEM/F12) ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Immunology 2023Quote: Processing of peripheral and cryopreservation was completed as previously described.8 Fresh tumor tissue was diced with a sterile scalpel and dissociated in 0.1% collagenase in RPMI 1640 for 60 minutes at 37⁰C using the gentleMACS OctoDissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... C-tubes tightly closed and attached upside down onto the sleeve of the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec.). The appropriate program 37C_ABDK_02 was run for 30 minutes ...
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bioRxiv - Genetics 2022Quote: ... anti-human-CD19 and anti-human-CD14 (Miltenyi Biotec) and transferred through LD columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cultures were grown overnight at 37 °C and shaking and then diluted in 1:50 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells3 ...
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bioRxiv - Plant Biology 2021Quote: ... Co-IP assays from transfected protoplasts were performed with C-Myc Isolation Kit (130-091-123) and DYKDDDDK (Flag) Isolation Kit (130-101-591) from Miltenyi Biotec. Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...