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Citations for Miltenyi Biotec :
301 - 350 of 719 citations for Human Carboxypeptidase M CPM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... followed by immunomagnetic separation using a human anti-CD14 purification kit (Miltenyi Biotec). The cell purity was always confirmed by flow cytometry and was superior to 95% ...
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bioRxiv - Biophysics 2023Quote: ... Monocytes were enriched from human PBMC by CD14 beads positive selection (Miltenyi Biotec) and cultured in IMDM supplanted with 10% human AB serum (omega scientific ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were then labelled with the human NK Cell Isolation Kit (Miltenyi Biotec), with subsequent NK cell isolation using the “depletes” program on an autoMACS Pro Separator and collecting the negative fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... PE anti-human MSCA1 (Clone W8B2, dilution 1/10, Miltenyi Biotec, Paris, France), APC antihuman CD271 (Clone ME20.4-1.H4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were activated with TransAct Human T cell activation reagent (Miltenyi Biotec) and 100 U/ml human IL-2 IS (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 50 IU/ml premium-grade human IL2 (Miltenyi Biotec, 130-097-746) at 1x106 cells per mL and cm2 ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
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bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].
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bioRxiv - Immunology 2022Quote: ... lungs of SARS-CoV-2 infected mice were collected and homogenized with gentle MACS M tubes (Miltenyi Biotec, 130-093-236) in a gentle MACS dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... 50mg of tumor tissue was disrupted in 1.5ml TRIzolTM using a gentleMACSTM dissociator and M tubes (Cat-No. 130-093-235 & 130-096-335, Miltenyi Biotec, Germany). RNA integrity was evaluated with Agilent Bioanalyzer 2100 and RNA 6000 Nano Kit (Cat-No ...
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bioRxiv - Microbiology 2020Quote: ... One gram of each tissue was homogenized in 9 ml of PBS in gentleMACS M tubes using the appropriate setting on the gentleMACS dissociator (Miltenyi Biotec). Homogenates were filtered through 40-μm-pore-size filters and an aliquot was used to determine viable counts ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... The organs were homogenized in 2 ml PBS using gentleMACS M tubes and gentleMACS Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Afterward ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was further supplemented with 5µM CHIR99021 (StemMACS, 130-103-926) on D2 and with or without 50 ng/ml hGdf11 (130-105-776, Miltenyi Biotec) on D3 ...
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bioRxiv - Cancer Biology 2022Quote: ... MDSC enriched bone marrow cells were collected from culture as described above and subjected to M-MDSC magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Each section was weighed and then homogenized in 2 ml PBS in gentleMACS M tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec) using the RNA_1 setting ...
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bioRxiv - Physiology 2021Quote: ... The muscle was removed from the -80 °C freezer and transferred to -20 °C where it was minced into pieces of 1-2 mm before being transferred to gentleMACS M-tubes (Miltenyi Biotec) tubes kept on ice ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Immunology 2021Quote: Mouse spleens and lymph nodes were removed from RNAlater solution and transferred to gentleMACS M tubes (Miltenyi Biotec 130-093-236) and 2 ml microtubes (OMNI 19-629-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor tissue was suspended in 35 uL of lysis buffer per mg of tissue and mechanically homogenized using M tubes (Milteny Biotec) with a gentleMACS Octo Dissociator (Miltenyi Biotec) using program “RNA_02” ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 20mg of tissue per sample was homogenized in RLT plus lysis buffer with 1% beta-mercaptoethanol using gentleMACS M-tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec). RNA extraction for both T-47D and PDX samples was performed using a Quick-RNA RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Cell Biology 2020Quote: ... Human ESCs were maintained in StemMACS™ iPS-Brew XF (iPS-B, Miltenyi Biotec) on Laminin-521 (L521 ...
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bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Human MSCs were cultured in StemMACS MSC Expansion Medium (Miltenyi Biotec, Bergisch Gladbach, Germany). Murine MSCs and LPR-MSCs were isolated and cultured as previously described (Albarenque et al. ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CD11b MicroBeads (mouse/human; Miltenyi Biotec Inc., Auburn, CA) and passed through MACS® LS magnetic columns for positive selection of CD11b+ cells ...
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bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
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bioRxiv - Immunology 2019Quote: ... filtered human AB serum then subjected to gentle mechanical dissociation using gentleMACS (Miltenyi Biotec) followed by short 20 min enzymatic dissociation at 37°C using a very low concentration of Liberase TL (0.042 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were incubated with anti-human CD14 antibodies coupled to magnetic beads (Miltenyi Biotec) and subjected to magnetic separation using LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Human and mouse lung tissues were digested using the Tumor Dissociation Kit (Miltenyi Biotec) for 30 minutes at 37°C and 1,500 rpm ...
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bioRxiv - Immunology 2020Quote: ... total PBMC were enriched in CD14+ monocytes using the human CD14 microbeads (Miltenyi Biotec) for positive magnetic selection according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were isolated using the human Pan T cell Isolation Kit (Miltenyi Biotec) and naive CD4+ T cell using the Naive CD4+ T Cell Isolation (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
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bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... PE CD158a/h (KIR2DL1/DS1) anti-Human antibody: 130-099-209 from Miltenyi Biotec. APC CD158b1/b2j (KIR2DL2/DL3/DS2 ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec, 130-120-709) for 1 h at room temperature in the dark in 0.75% saponin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... human cells were incubated with anti-biotin microbeads (Miltenyi Biotec, Cat#130-090-485) in MACS buffer at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... All setups were stained with 5μL of human FcR blocking reagent (Miltenyi Biotec, USA) to mitigate unspecific binding and LIVE/DEAD blue stain (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Human bone marrow CD45+ cells were sorted by immunomagnetic selection with AutoMACS (Miltenyi Biotec) after immunostaining with the CD45 MicroBead Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... and CD38 (20 µl per 107 cells, from human CD38 MicroBead kit, Miltenyi Biotec) followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 100 ng/mL of recombinant human flt3 ligand (Miltenyi Biotec, Auburn, CA), stem cell factor (SCF ...
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bioRxiv - Microbiology 2022Quote: ... CD8 T cells were depleted using a kit from Miltenyi Biotec (CD8 MicroBeads, human) and cells cultured in RPMI-1640 (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Enzymes and reagents from the human tumor dissociation kit (Miltenyi Biotec: 130-095-929) were added to the tumor tissue along with 1.75 mL of DMEM ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Immunology 2024Quote: ... Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec). Monocytes were then plated 0.15×106/well onto 96-well plate (for ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Thawed and fresh samples were dissociated using the Tumor Dissociation Kit (human, Miltenyi Biotec) and tough dissociation protocol on gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...