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Citations for Miltenyi Biotec :
1 - 50 of 311 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Immunology 2020Quote: ... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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bioRxiv - Immunology 2019Quote: ... antigen-specific T cells were separated using the CD154 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s suggestions ...
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bioRxiv - Microbiology 2021Quote: ... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8 T cells were isolated from the spleen of pmel mice (having CD8 T cells bearing T cell receptors specific for the gp100 antigen) by magnet-assisted cell sorting (Naïve CD8+ T cell isolation kit, Miltenyi Biotec) and stained with Cell Trace Violet (CTV ...
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and suspensions were incubated with magnetic microbeads targeting the uNK specific antigen CD56 (#130-050-401, Miltenyi Biotec, Bergisch Gladbach, Germany) diluted 1:20 in wash buffer (1% BSA/PBS ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Immunology 2024Quote: ... Purified CD154+ T cells were expanded in presence of 1:100 autologous antigen-loaded irradiated feeder cells in TexMACS medium (Miltenyi Biotec), supplemented with 5% (v/v ...
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bioRxiv - Genomics 2019Quote: ... The tissue lysate was then filtered through gentleMACS SmartStrainers (100 μm) (Miltenyi Biotec) and 200 μL of the lysate was sampled and mixed with 700 μL of QIAzol for total RNA extraction ...
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bioRxiv - Systems Biology 2023Quote: ... the tissue lysate was incubated with CD146 microbeads (Miltenyi Biotec, 130-092-007) before being passed through magnetic columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... On day4 transduced cells were positively selected by magnetic assisted cell sorting (MACS) via the cell surface antigen CD90.1 (Thy1.1) using microbeads (Miltenyi Biotec: 130-094-523); the manufacturer’s protocol was adjusted by using 4x less reagents and by maintaining cells at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Biochemistry 2021Quote: ... Liver lysates were prepared in MDEG buffer by homogenization using the gentleMacs Dissociator (Miltenyi Biotec), followed by ultracentrifugation at 100,000xg and 4°C for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... Cell lysate extracts were incubated with the anti-GFP microbeads (Miltenyi Biotec GmbH, Gladbach, Germany) for 1h ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded to a MACSQuant Tyto HS Cartridge (#130-121-549, Miltenyi Biotec) and sorted according to the instrument instructions until 1 x 107 positive events had been collected ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Microbiology 2021Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to electroporation.
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bioRxiv - Microbiology 2022Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to infection.
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). All HTLV-1 infected T-cell clones were maintained in 10 µM of the integrase inhibitor ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike peptides (PepTivator SARS-CoV-2 Prot_S, Miltenyi Biotec) were added at a final concentration of 0.6 nmol/ml in the presence of 1 μ (total cell culture volume ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% B21 (Miltenyi biotec), 1% P/S (Sigma ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Microbiology 2023Quote: ... penicillin (100 U/ml) and interleukin 2 (IL-2, Miltenyi Biotec #130-097-745) (10 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM CHIR99021 (Miltenyi Biotec), 0.5 μM A83-01 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μM Purmorphamine (Miltenyi Biotec), 100 ng/ml FGF8 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec) and TNF-α-APC (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 ng/mL Fibroblast Growth Factor 2 (FGF-2) (Miltenyi Biotec, 130-105-787), for 14 days with medium change every 2 days ...
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bioRxiv - Microbiology 2024Quote: ... Splenocytes were stimulated with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Catch Reagent (Miltenyi Biotec), IL-4 Catch Reagent (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... such as IWP-2 (Miltenyi Biotec). On day 5 ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: 10 μl of anti-ACSA-2 MicroBeads (Anti-ACSA-2 MicroBead Kit, # 130-097-678, Miltenyi Biotec) and an additional incubation for 30 min ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were stimulated with SARS-CoV-2 peptide pools of S and N (2 μg/ml) (Miltenyi Biotec) in the presence or absence of CECs ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 12 ng/ml FGF-2 (Miltenyi Biotec) and 20 ng/ml Activin A (PeproTec ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Miltenyi Biotec, 130-097-745) was supplemented (100 unit/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... + 2% BSA (Miltenyi Biotec: 130-091-376) + 0.2 U/μL RNase inhibitor ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... or the PepTivator SARS-CoV-2 Prot_N peptide mix (covering the complete sequence of the SARS-CoV-2 N protein; both Miltenyi Biotec). As controls splenocytes were either left unstimulated or activated polyclonally with 12.5 µg/mL phytohemagglutinin (PHA) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in 8 ml cold PBE buffer (PBS with 0.5% BSA and 2 mM EDTA) and 2 ml anti-PE microbeads (Miltenyi Biotec) per 108 cells and incubated for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... NKM-PE (16-2-4) from Miltenyi Biotec, Vγ7-FITC (kindly provided by P ...
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bioRxiv - Immunology 2020Quote: ... and 100 U/ml hIL-2 (Miltenyi Biotec) and cultured in IMDM media (supplemented with 10 % FBS ...
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bioRxiv - Neuroscience 2021Quote: ... ACSA-2-PE (Miltenyi Biotec, 130-102-365), Mouse Fc blocker (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... and hIL-2 (50 IU/ml, Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... and IL-2 (100 IU/mL; Miltenyi Biotec) in X-VIVO 15 medium (Lonza) ...