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Citations for Miltenyi Biotec :
501 - 550 of 889 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and FcR blocking reagent (Miltenyi Biotec, dilution 1:5) or isotype control antibody (mouse IgG1 ...
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bioRxiv - Cell Biology 2021Quote: ... and MEFSK4 (1:50 Miltenyi Biotec 130-120-802). This allows for the removal of CD11b cells which are known to express high levels of MBNL1 ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... and anti-c-myc-FITC (1:50, Miltenyi Biotec, cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec), 1× MEM NEAA (GIBCO™) ...
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bioRxiv - Immunology 2024Quote: ... Cells were activated with TransAct (1:150, Miltenyi Biotec) or PBS (negative activation control ...
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...
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bioRxiv - Immunology 2020Quote: ... from the spleen of naive mice after enrichment of CD11c+ cells using a PE-conjugated anti CD11c antibody and magnetic-activated cell sorting (Anti-PE MicroBeads, Miltenyi Biotec). For in vitro functional analysis ...
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bioRxiv - Immunology 2019Quote: ... Neutrophils were counted using an Invitrogen automated cell counter and the purity assessed by flow cytometry using FITC anti-CD16 and APC anti-CD15 antibodies (Miltenyi Biotec) as markers characteristic of human neutrophils.
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bioRxiv - Physiology 2019Quote: ... Resulting stromal vascular fractions were used to isolate ATMs using mouse anti-F4/80-FITC antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany), anti-FITC MicroBeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: 2 × 105 peritoneal exudates cells were stained with fluorochrome-conjugated antibodies (anti-mouse) directed against the T-cell subset surface marker CD4 (Biotin, Miltenyi Biotec), the alpha chain of the IL-2 receptor CD25 (PE ...
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bioRxiv - Microbiology 2020Quote: ... BMDCs were enriched by depletion of BMMs using biotinylated α-F4/80 antibody (eBioscience, RRID: AB_466657) and anti-biotin conjugated magnetic beads (Miltenyi Biotec). The BMDCs in the flow through were positively selected using α-CD11c magnetic beads according to the manufacturer’s protocol (Mil405 tenyi Biotec).
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bioRxiv - Immunology 2019Quote: ... or sorted using a FACSAria II (Becton Dickinson) flow cytometer after antibody staining and lineage selection or depletion using anti-biotin beads and LS columns (Miltenyi Biotec). Data was analysed using FlowJo software (Version 8.8.7 ...
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bioRxiv - Cancer Biology 2021Quote: Murine liver primary ECs were isolated from athymic nude mice also using MACS microbead-conjugated antibodies for murine CD31 (Miltenyi Biotec) and cultured in MV2 EC culture medium as described above ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... thymocytes were isolated from adult mice by negative magnetic selection using biotinylated anti-CD4 and anti-CD8 antibodies and streptavidin (SAV) microbeads (Miltenyi Biotec). To isolate fetal progenitor cells ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and stained with fluorophore conjugated antibodies (CD11b-AF700, CD45-FITC, BD Pharmingen 557690 and 553080, C5aR-PE, CD68-PE and CD107a-PE, Miltenyi Biotec 130-106-174 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... slides were incubated for 1 h at room temperature in the dark with a cardiac troponin-T antibody conjugated to APC (130-120-543; Miltenyi Biotec) prepared at a 1:100 dilution in PBS ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were enriched from the filtrates by Ficoll density gradient centrifugation and magnetic-activated cell sorting using CD34 antibody-conjugated microbeads (Miltenyi Biotec). CD34+ cells were grown in serum-free StemSpan SFEMII (Stemcell Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec, and employed at concentrations recommended by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34 ...
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bioRxiv - Genomics 2021Quote: ... with fluorescent APC-conjugated anti-CD14 and PE-conjugated anti-CD16 antibodies (Cat#130-091-243 and Cat #130-091-245, respectively, Miltenyi Biotec). Samples were then analyzed on a MACSQuant Analyzer 10 benchtop flow cytometer (Miltenyi Biotec).
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bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs) for either the 8-color MACSQuant Analyzer (Miltenyi Biotec), or the 14-color LSRFortessa™ X-20 flow-cytometers (BD) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human B cells from PBMC were labelled with B Cell lineage-specific CD19 antibodies and removed by magnetic-activated cell sorting by using the Lineage Cell Depletion Kit for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Immunology 2020Quote: ... CD137-expressing CD4+ memory T cells cells were positively selected by staining with biotin-conjugated CD137 antibody (clone REA765; Miltenyi Biotec) followed by anti-biotin MicroBeads and applied to a new MS column ...
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bioRxiv - Immunology 2021Quote: ... Cells were recovered via centrifugation and stained as described above using a biotinylated monoclonal antibody against TIM4 (Miltenyi Biotec, clone: REA999), the isolated with anti-biotin Microbeads (Miltenyi Biotec ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Cell Biology 2022Quote: ... Magnetic enrichment was carried out on an AutoMACS using microbeads conjugated to antibodies recognizing biotin or mouse CD117 (all from Miltenyi Biotec). HSCs (CD45.2+ ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were enriched by MACS using an in-house biotinylated anti-EGFR antibody (Cetuximab, Eli Lilly and Company, Indianapolis, IN, USA) and anti-Biotin Microbeads (Miltenyi Biotec). Purity of enriched CAR modified T cells was analyzed as described above ...
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bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Molecular Biology 2023Quote: ... Negative controls were incubated with fluorochrome-labeled irrelevant isotype control antibody (REA Control APC, mouse cod. 130-113-446 Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with FACS Intracellular Staining Perm Wash buffer and the following antibodies were added to the cells: anti-STAT1 pTyr701 (Miltenyi Biotec), and anti-STAT3 pTyr705 (eBioscience™) ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells ...
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bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: ... Human lung single T cells were prepared from surgical resection and lungs in patients as previously described by selection with biotin-labeled antibodies by autoMACs (Miltenyi Biotec) (Yuan et al. ...
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bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
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bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101, Miltenyi Biotec). CD4+ T cell enrichment was confirmed by determining the percentage of CD3+/CD4+ cells via flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... human T-ALL cells were purified after labeling with anti-huCD45 PE conjugated antibody followed by immuno magnetic selection with anti-Phycoerythrin (PE) microbeads (130-048-801, Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25- Tcon were isolated from the CD25- fraction obtained from the same samples by negative selection using FITC-conjugated antibodies and anti-FITC Microbeads (Miltenyi Biotec) as described above (T cell isolation) ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...